Posted by admin on Jan 31, 2022 in |
Current analytical research and experiments provide the development of a simple, sensitive, accurate, rapid, and stability indicative RP-UPLC method for estimation of Levocetirizine in Levocetirizine dihydrochloride tablets. This development was achieved using a Waters Acquity UPLC with BEH, C18 column of dimensions of 100 mm × 2.1 mm, 1.7 µm column at 0.3 mL/min flow rate and Acquity TUV detector at 230 nm. This method was validated based on the guidelines of the International Council on Harmonization (ICH). Linearity was demonstrated at 25% to 150% levels with R2 value of 0.999. Precision and accuracy were in line with the ICH guidance with a mean recovery of 99.02%. The RP-UPLC method is sensitive with levels of Limit of detection (LOD) and Limit of Quantitation (LOQ) at 0.04µg/mL and 0.12µg/mL, respectively. Degradation studies in conditions of Oxidation, Acid, Base, Temperature, Water, UV light demonstrate there is no placebo as well as degradation impurities interfering with the Levocetirizine main peak. The simple, sensitive, accurate, rapid, and stability-indicating method makes it an efficient...
Read More
Posted by admin on Jan 31, 2022 in |
Multi particulate systems are of greater importance than the single unit dosage forms in oral drug delivery. Floating micro balloons are among the best methods to increase gastric retention among all the multi particulate drug delivery systems. They offer more reproducible drug absorption, reduce the risk of local irritation and improve the bioavailability of the drug. In the current research work, clopidogrel, a BCS class – II drug, was formulated as controlled release micro balloons using ethyl cellulose as polymer and span 80 as the surfactant. The pre-formulation studies viz. solubility, partition coefficient, micromeritics were carried for the Clopidogrel bisulphate pure drug, and the drug excipient compatibility studies were carried using Fourier transform infrared spectrophotometer and Differential Scanning Calorimeter. Different formulations of floating micro balloons were prepared by solvent evaporation method by altering five process and formulation factors viz. concentration of surfactant, the volume of solvent, volume of internal phase, the concentration of polymer, speed of rotation. All the Clopidogrel micro balloons were subjected to in-vitro drug release and...
Read More
Posted by admin on Jan 31, 2022 in |
Amantadine hydrochloride (1) is an antiviral drug currently used to treat certain influenza infections (type A). It is also used as an anti-dyskinetic agent to treat Parkinson’s disease. Several methods for the preparation of amantadine hydrochloride (1) were reported, these procedures started from adamantane (2), using procedure four reaction steps to obtain amantadine hydrochloride with an overall yield of 46-58%. In this article, we present a convenient two-step procedure for the synthesis of 1 from 1-bromoadamantane (3) and acetylamide in the presence of sulfuric acid via N-(1-adamantyl) acetamide (4) in one-pot with an overall yield of 74%. The procedure was also optimized to reduce the use of toxic reagents and solvents to make it to safer and more environmentally friendly. The procedure can be considered as more suitable for large-scale production of amantadine hydrochloride. The structure of the obtained amantadine hydrochloride was confirmed by MS, IR, 1H-NMR and...
Read More
Posted by admin on Jan 31, 2022 in |
Paramphistomiasis is the major problems in the yield of goat and human health. This disease causes loss of life of goat, reduce meat and milk production. The medicinal plant Capparis decidua has been used in the treatment of asthma, cough and inflammation. This plant also shows anthelmintic activity. Live amphistome Gastrothylax crumenifer were collected from the rumen of the freshly slaughtered goats at local meat market of Chittorgarh. Amphistomes Gastrothylax crumenifer was maintained in 0.9% physiological saline and divided into four groups; first group used for identification, second group as control or untreated, third group for treatment with Capparis decidua and fourth group for treatment with synthetic drug albendazole. The histological study of treated and control tegument of Gastrothylax crumenifer was observed by light microscopy. Extract of Capparis decidua caused many topographical changes in Gastrothylax crumenifer like discontinuation, distortion, peeling and damaging cells, vacuolization and deformation in the muscles fiber, found blebblings in tegument. Albendazole is less effective than alcoholic extract of Capparis decidua (Kair). This study suggests the...
Read More
Posted by admin on Jan 31, 2022 in |
Independent studies have shown that chalcones and 123 triazole exhibited cytotoxic effects on human cancer cell lines. In the present study, we prepared a series of novel 1,2,3-triazole-chalcone hybrid compounds and evaluated the cytotoxic effect and probable mode of action in HT29 colon cancer cell line. Among the 10 evaluated compounds, compounds 4 (3-nitro) and 8 (3-chloro) were the only compounds that elicited a dose-dependent decrease in cell viability (IC50 values 9.7 and 33 M, respectively) of HT 29 cells. The same compounds exhibited a significant inhibitory effect on the cell proliferation and colony-forming capability of HT29 cells. Co-administration of compounds 4 and 8 with electron transport chain inhibitors and mitochondrial K+-ATP channel blockers in H9C2 enhanced the anti-proliferative capacity of the compound. Furthermore, Insilco molecular docking studies revealed that the anti-proliferative effect of these compounds could be attributed to interactions with the colchicine-binding site of tubulin. In conclusion, our results demonstrated that novel 1,2,3-triazole-chalcone hybrid can be a potent therapeutic agent against HT-29 cells, and its anti proliferating...
Read More
