Posted by admin on Nov 30, 2021 in |
The goal of the research was to explain as a cognition enhancer the traditional claim of Madhuca longifolia (Sapotaceae) leaves. Dementia was activated by intracerebrovascular colchicine administration for 28 days. Madhuca longifolia leaves ethanolic extract was evaluated in mice for cognition-enhancing activity in colchicine-induced dementia using elevated plus maze (EPM), actophotometer, and biochemical estimation of brain AChE and MDA levels. Piracetam (200 mg/kg p.o.) was used as standard medicine. Transfer latency (TL) was observed in the colchicine-induced mice using EPM, showing a significant increase (p<0.01) in TL compared to the regular control group. No major effect was found on the locomotor activity of the mice. In colchicine-induced mice, a significant increase (p<0.01) in AChE and a decrease in MDA levels relative to normal control were observed. These results formed the standard argument, and Madhuca longifolia can therefore be a memory enhancer because of the presence of...
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Posted by admin on Oct 31, 2021 in |
Esmolol Hydrochloride belongs to the class II anti-arrhythmic drugs. Due to composite compositions and low reproducibility, challenges are still warranted for the systematic method development for related substances and identification of its acid degradation products. Development, validation and characterization of RP-HPLC stability-indicating method for the quantitative analysis of two impurities in Esmolol hydrochloride and its stress degradants. The validation of this method was achieved as per ICH Q2 (R1) guidelines with the optimized experimental conditions. To achieve the proposed method on C18 (250 mm × 4.6 mm, 5µ) column and the temperature was sustained at 30°C and run time was 12 min. The mobile phase consists of A–Acetonitrile, B–0.01N Potassium dihydrogen orthophosphate in water (pH=4.8). The injection volume of samples was 10μL, and UV detection was carried out at 221nm. Linearity ranges were covered 0.25-1.5 ppm for ASL-8123, ACC-9675 and Esmolol Hydrochloride. The newly developed method for separation of impurities along with the pure drugs was found to be capable of giving swift retention times while still preserving good...
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Posted by admin on Oct 31, 2021 in |
A reverse-phase liquid chromatography (LC) method was developed for the simultaneous estimation of enrofloxacin and its active metabolite ciprofloxacin residues in chicken meat. These two fluoroquinolones were extracted from homogenized tissues with phosphate buffer under acidic conditions, followed by acetonitrile. The isocratic LC analysis was performed on Phenonmenax, C18 RP column (250 × 4.6 mm, 5 μ) using a mobile phase composed of 0.025M potassium phosphate solution (pH 3.25) and acetonitrile in the ratio of 75:25 (v/v), at a flow rate of 0.500 ml/ min. Quantitation of enrofloxacin and its metabolite were performed using a PDA detector set at the wavelength of 277 nm. The temperature of the column was 300C, and the total run time was 20 min for the analysis of both drugs. The retention time was found to be 10 min for ciprofloxacin and 13 min for enrofloxacin. The r2 values for linearity studies were greater than 0.98, and the accuracy (% recovery) was greater than 88% for both the drugs. The linearity was observed in...
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Posted by admin on Oct 31, 2021 in |
Berberine, an isoquinoline alkaloid that has better anticonvulsant and antioxidant activities, results in less efficacy due to poor solubility, poor permeability, poor stability and poor drug delivery to the brain. Hence, this study aim at improving Anticonvulsant and Antioxidant activities of Berberine by formulating nanoparticles with three-factor two-level boxbehnken with design expert software 6.1. In this study, the particle size and drug release percentage were considered as dependent variables, whereas the concentration of HPMC, PEG and Sonication time was considered as independent variables. Similarly, sodium caprate was separately optimized by Franz diffusion study to enhance the permeability, and thus nanoparticles were formulated with solvent evaporation method. Prepared nanoparticles were characterized and evaluated. FTIR, DSC and the Computer-based drug interaction revealed that drug encapsulated with HPMC and PEG was compatible with each other. Developed nanoparticles were found to be below 500nm. Zeta potential of nanoparticles was -35.9mV which indicated that the nanoparticles were stable; MES study, Rotarod study, and biochemical study results revealed that the anticonvulsant activity and antioxidant activity...
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Posted by admin on Oct 31, 2021 in |
Introduction: In this present study, the heaptoprotective effect of an optimized herbal formula (HF) was evaluated against carbon tetrachloride (CCl4)-induced liver injuries in rats. Methods: In-vitro antioxidant activity of the HF was evaluated by reducing power, lipid peroxidation, and DPPH scavenging assays. Hepatoprotective activity of HF (100, 200 and 400 mg/kg b.w) was evaluated against CCl4-induced hepatic damage in rats. Serum enzymatic levels of serum glutamate oxaloacetate transaminase (AST), serum glutamate pyruvate transaminase (ALT), serum alkaline phosphatase (ALP), and Gamma-glutamyltransferase (γ-GT) were estimated along with an estimation of catalase (CAT), superoxide dismutase (SOD), Glutathione (GSH) and TBARS levels in liver tissues. The lipid profile was also examined. Furthermore, histopathological examination of the liver sections and p53 expression assay were performed to confirm and advocate the induction of hepatotoxicity as well as hepatoprotective efficacy. RESULTS: The HF exhibited strong in-vitro antioxidant activities in terms of reducing power, inhibition of lipid peroxidation, and DPPH radical scavenging. HF restored the significantly elevated serum enzymatic levels of AST, ALT, ALP and γ-GT in...
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