Posted by admin on Aug 31, 2021 in |
Bio behavioural aspects, immunity, and disease course are interlinked in a majority of conditions, particularly cancer. Peripheral stressors or their products regulate immunity by various ways. When there is added insult in an already compromised immune state such as cancer, as is done here using lipopolysaccharide (LPS), the system is said to be ‘double-hit. Behavioural and brain tissue-associated biochemical changes on LPS challenge have been assayed in a Diethylnitrosamine (DEN)-treated Wistar rat model of hepatic cancer. Peripheral and central effects of LPS challenge have been screened at levels of neuro behaviours, liver enzymes and brain enzymes. While LPS challenge reduced exploratory and grooming behaviours across groups after 2 h, the effect disappeared on testing after 4 h, 6 h post LPS injection, rats demonstrated increased open arm time, reduced closed arm time and increased entries into both arms of the elevated plus-maze. DEN treatment induced similar results. LPS challenge in DEN-treated rats however, led to reduced open arm time, increased closed-arm time, and reduced entries into both arms. LPS...
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Posted by admin on Aug 31, 2021 in |
A RP-UFLC method was developed &validated for simultaneous estimation of Sitagliptin Phosphate and Metformin HCl in bulk and tablet dosage form. Separation was achieved by using Phenomenex Luna C18 (150 × 4.6mm, 5µm) column. The mobile phase comprises MeOH: Phosphate Buffer of pH 7 using Triethylamine in the ratio (60:40v/v). The flow rate was 0.6ml/min at temperature 300C. The instrument used for UFLC was Shimadzu autosampler separation model LC 20AD. Quantification was achieved with UV detection using a photodiode array detector at 258nm wavelength. The injection volume was 20µl. The retention times of Sitagliptin Phosphate & Metformin HCl were found to be 7.027 & 2.802, respectively. The linearity range of Sitagliptin Phosphate was 50-100µg/ml & Metformin HCl was 10-60µg/ml. The correlation coefficient was found to be within limits, i.e., 0.999. The % purity of Sitagliptin Phosphate & Metformin HCl was found to be 101.99% and 101.73%. LOD values were found to be 2.21437 for Sitagliptin Phosphate & 1.99084 for Metformin HCl and LOQ values were found to be 6.71024...
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Posted by admin on Aug 31, 2021 in |
Marine sponges have become a promising source of secondary metabolites with diverse structures and have been shown to have a wide range of biological activities. In search of bioactive compounds, a preliminary cytotoxic assessment of marine sponge extracts was conducted. The cytotoxic activities of the hexane, dichloromethane (DCM), ethyl acetate, and aqueous extracts of the marine sponges, Stelodoryx procera Topsent 1904 and Rhabdastrella sp., collected off the coast of Zamboanga City, Philippines were tested using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction assay. The hexane extract, ZP12NPh, from S. procera and DCM extract, ZP07NPd, from Rhabdastrella sp. exhibited moderate toxicity against colorectal cancer (HCT116) cell line at 30 µg/mL. Further purification of the hexane extract from S. procera has afforded two (2) sub-fractions, ZP12NPh4 and ZP12NPh5, which have shown to be highly cytotoxic against HCT116 at 30 mg/mL. While the DCM extract from Rhabdastrella sp., has afforded fourteen (14) highly cytotoxic sub-fractions (ZP07NPd2, ZP07NPd3, ZP07NPd4, ZP07NPd5, ZP07NPd9, ZP07NPd10, ZP07NPd13, ZP07NPd15, ZP07NPd16, ZP07NPd17, ZP07NPd18, ZP07NPd19, ZP07NPd20 and ZP07NPd21) against HCT116 at...
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Posted by admin on Aug 31, 2021 in |
A simple and precise stability-indicating reversed-phase (RP-HPLC) method was developed and validated for the determination of Apixaban active pharmaceutical ingredient. The chromatographic condition was carried out on Phenomenex Luna 5 µm C18 100 Å (150 × 4.6 mm) HPLC column with prepared buffer solution 1.0 ml of Orthophosphoric acid mixed with 0.2% of potassium dihydrogen phosphate solution, use as mobile phase (a), mobile phase (b) was acetonitrile. The mobile phase flow rate at 1 ml/min and the detection wavelength is 230 nm. Forced degradation study was covered acid hydrolysis, base hydrolysis, peroxide, photolytic (UV light) and thermal degradation was performed to prove the specificity of the proposed method and degradation was achieved. The developed method has been statistically validated according to ICH guidelines and found to be simple, precise, linear and accurate with the prescribed values. Thus the proposed RP-HPLC method was successfully applied for the substance in routine quality control analysis in active pharmaceutical...
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Posted by admin on Aug 31, 2021 in |
The aim of the present study was to improve the release rate of herbal drugs by microsponges prepared through quasi-emulsion solvent diffusion technique using ethyl cellulose and PVA as carriers. The microsponges were characterized by FTIR, DSC and SEM studies followed by the determination of total drug content and entrapment efficiency. Anti-arthritic activity was evaluated by Freund’s Complete Adjuvant (FCA) induced arthritic rat model. RA was induced by intradermal injection of 0.1 ml FCA in the left hind paw. The animals were treated with doses 50:50, 70:30, 30:70, and 100:100 mg/kg b.w. of microsponge formulation. Methotrexate at a dose of 10 mg/kg is used as a standard drug. Assessment of paw volume, % inhibition of paw volume, serum parameters for RA factor determination and histopathological examination was also carried out.In this current study, the microsponge formulation (B. serrata + A. barbadensis) had a better effect on controlling CFA induced arthritis. Formulation codes M1 and M4 showed a good reduction in paw edema and joint thickness. It produced a significant...
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