Posted by admin on Nov 30, 2024 in |
Background: Fungi have emerged as major cause of human diseases since early 1980s. In hospitalized patients with serious underlying diseases and those having immunocompromised state, opportunistic fungal infections bring about a substantial increase in morbidity and mortality. Aim and Objectives: Isolation and characterization of Candida species in ICU patients of Dr. Susheela Tiwari Government Hospital, Haldwani, Uttarakhand. Materials and Methods: A prospective observational study was conducted for two years from Nov 2015 to Oct 2017 and 2323 samples were collected from suspected cases of Candida infection, admitted in ICU of Dr. Susheela Tiwari Government Hospital, Haldwani. Identification of Candida species was done by standard laboratory processes. Results: In the present study, out of 2323 clinical specimens received from different ICU’s, 104 samples were found positive for Candida species. Majority of the patients were in the age group ˃ 60 years (24.04%). We observed that Non-albicans Candida (NAC) species (67.3%) were more frequently encountered than C. albicans (32.7%). Among NAC, C. tropicalis was the most common species (61.4%). Maximum numbers...
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Posted by admin on Nov 30, 2024 in |
The design and synthesis of a novel 2,4,5- Triphenyl imidazole derivative has been achieved using a green chemistry approach. These compounds were synthesized by using a sustainable catalytic material and solvent free one-pot reaction where benzil, aromatic aldehydes, ammonium acetate and acid chloride were reacted under microwave irradiation. This method offers several advantages including simplicity, high yields, and short reaction times, making it more environmentally friendly and alternative to conventional methods. The synthesized compounds were characterized by determination of melting point, thin layer chromatography and Fourier transformer infrared spectra. Molecular docking studies were performed to evaluate the binding affinity of the derivative with the proteins 4XMB (Crystal structure of 2, 2′-(naphthalene-1, 4-diylbis (4-methoxyphenyl) sulfonyl) azanediyl) diacetamide bound to human Keap1 Kelch domain)) and 6BIV (HLA-DRB1 in complex with citrullinated LL37 peptide). The docking results indicated that the novel 2,4,5-triphenyl imidazole derivative has significant binding affinity with the protein 4XMB and showed poor binding affinity with the protein...
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Posted by admin on Nov 30, 2024 in |
The present investigation aimed to design a biodegradable plastic from soil microorganisms that produce PHB (Polyhydroxy-butarate). The microorganism was isolated using a specifically designed medium as per the requirement and identified the organism by Sudan black and gram staining methods. Their biochemical identification was done by the Indole test. Then extraction of PHB was done through the centrifugation method where a pellet was used to make biodegradable plastic. Four different types of microorganisms were isolated, from which pseudomonas species were selected and produced in large quantities for the production of PHB, which is produced by bacteria under stressed conditions. The indole test was positive, the gram nature of the bacteria is negative and the shape is rod. The formation of plastic film was successfully done. The results obtained in this research work confirm that biodegradable plastic can be obtained from agricultural microorganisms also. Thus, the plastic film is easily degraded within a short...
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Posted by admin on Nov 30, 2024 in |
The main objective of this study was to improve the solubility of the triterpenoid cabraleone (DN12) and to investigate the anti-inflammatory activity of the complex. DN12 was stabilized by a complex formation with β-cyclodextrin polymer (β-CD-P) in a molar ratio of 1.0:1.0. The DN12 extract and the cyclodextrin complex have been equilibrated in ethanol at 25°C for approximately 24 h. The β-cyclodextrin-polymer complex (β-CD-P-DN12) was characterized by a combination of experimental methods including ultraviolet-visible spectroscopy, differential scanning calorimetry, thermogravimetric analysis, X-ray diffraction, infrared and nuclear magnetic resonance. The formation of the complex with β-cyclodextrin-polymer was confirmed by the use of five analytical tools. In-vivo anti-inflammatory activity, using 1 % of the carrageenan-induced rat paw oedema method of the β-CD-P-DN12 complex compared to that of DN12 alone was studied. The results showed that inclusion complex formation β-CD-P-DN12 improved stability and solubility in aqueous solution compared to free DN12. The results of the in-vivo study showed that the β-CD-P-DN12 inclusion complex exhibited different pharmacokinetics as compared to free DN12 after injection...
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Posted by admin on Nov 30, 2024 in |
Ionotropic gelation, a characteristic property, has become one of the easy, simple and mild ways of encapsulating variety of drugs, enzymes, and proteins, prepared by various Polysaccharides. The externally crosslinked beads with calcium chloride and zinc chloride were suitable for water soluble and low molecular weight drugs. Insulin, encapsulated in a calcium pectinate gel / Sodium alginate bead by dispersing the insulin and core cross linking agent in a solution of pectin, beads are prepared by spraying the dispersion in calcium chloride by Inotropic gelation. The purpose of present research work was to study effect of different concentration of sodium alginate and pectin solution on the drug entrapment, drug release and stability of Insulin. Insulin is a high molecular weight drug, widely used to treat diabetic patients. The beads were prepared with factorial design and evaluated for, entrapment efficiency, stability and bioadhesivity. The formulated gel was evaluated for administration in the nasal cavity for treatment of...
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