Posted by admin on Aug 31, 2021 in |
Background: Dolutegravir and Rilpivirine are two antiretroviral drugs that have been approved for the treatment of HIV Infection. An error-free, accurate, precise and valid reverse-phase liquid chromatography method was developed for the quantitation of Dolutegravir and Rilpivirine in its bulk form as well as in combined dosage form by forced degradation studies. Methods: Chromatographic separation of these two drugs Dolutegravir and Rilpivirine, was achieved with an INERTSIL ODS C18 (250 × 4.6 mm, 5 μm) reverse-phase analytical column with a 10 min analytical run time using a mixture of 0.1% OPA: Acetonitrile in the ratio of (60:40 v/v) as mobile phase. The mobile phase was streamed at a flow rate of 1.0 mL min-1 with a column temperature of 250 °C and detection wavelength was carried out at 230 nm. The retention time was found to be 3.4 min for Dolutegravir and 4.3 min for Rilpivirine. Results: The linearity limit of Dolutegravir and Rilpivirine was found to be in the range of 0.999 and 0.999. The method validation was...
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Posted by admin on Aug 31, 2021 in |
Zingerone is an active phenolic acid and the least pungent component of Zingiber officinale. It is known to exhibit different pharmacological properties which include anti-inflammatory, antioxidant, anticancer and antimicrobial activities. But, so far there has been no information available on the role of zingerone in experimental breast carcinogenesis. The objective of this experiment is to untwine the anticancer qualities of zingerone against dimethylbenz (a) anthracene (DMBA)-incited mammary carcinogenesis. To achieve this goal, female Sprague-Dawley rats were arbitrarily classified into six groups. Group 1 was notified as the control, groups 2-5 were given a single dose of DMBA (25 mg/kg b.wt, subcutaneously) in the 4th week. Along with DMBA, groups 3 (initium), 4 (post-initium) and 5 (entire period) rats received zingerone (20mg/kg b.wt. p.o) every day in different time periods during the experimental period of 16 weeks. Group 6 rats received 20 mg/kg b.wt. of zingerone alone. Cancer-bearing animal mammary tissue when evaluated by western blot showed increased immune expression patterns of ER, PR, HER2/ neu, cyclin D1 and also...
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Posted by admin on Aug 31, 2021 in |
One of the major barriers in the development of oral dosage form is poor solubility. Poor water solubility obstructs drug bioavailability and decreases its pharmaceutical development. Many drugs are in the pipeline to enhance solubility to formulate dosage form to be taken by the most preferred route of administration that is the oral route. Etodolac is nonsteroidal anti-inflammatory drug having a wide spectrum of activities but belongs to BCS class II. The attempt has been made in this work to improve solubility by forming ternary inclusion complexes of Etodolac with PVP K30 and β-Cyclodextrins. The ternary inclusion complexes were prepared by the physical mixing method and kneading method. The prepared complexes were analyzed by different analytical techniques comprising differential scanning calorimetry, infrared spectroscopy and solubility study. Special emphasis was given on the solubility evaluation of drugs and complexes. Based on observations and results, one can easily conclude about the usefulness of the complexation technique for the enhancement of...
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Posted by admin on Aug 31, 2021 in |
Quinoline or 1-aza-naphthalene or benzo [b] pyridine is a weak tertiary base. In the study, the synthesis of compounds and their antimicrobial activity which is not done earlier and is quite significant. An attempt has been made for the synthesis of N’-(substituted benzylidene)-2-(7-bromo-2-phenylquinazolin-4-yloxy) acetohydrazide (5-15). The titled compounds were prepared by the reaction of bromoanthranilic acid with benzoyl chloride which gave oxazine-4-one derivative (1), which on reaction with formamide gave quinazolin-4(3H)-one derivative (2). The esterification product of quinazolin-4(3H)-one derivative, when reacted with hydrazine-hydrate gave (7-bromo-2-phenylquinazolin-4yloxy) acetohydrazide (4). The sub stituted benzaldehyde on reaction with 7-Bromo-2-phenylquinazoline-4yloxyacetohydrazide (4) yielded N’-(sub stituted benzylidene)-2-(7-bromo-2-phenyl quinazolin-4-yloxy) acetohydrazide (5-15). Primarily the structures of all synthesized compounds were confirmed from Melting point and TLC methods and then spectral analysis IR, 1H NMR and mass spectra. All synthesized derivative compounds were evaluated for their in vitro antimicrobial activities using the disc diffusion technique. It was found that all the synthesized compounds exhibit antimicrobial activity and that compounds 7, 11, 13 and 14 have a broad spectrum of...
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Posted by admin on Aug 31, 2021 in |
The study aimed to analyze the treatment of Sitagliptin with Glimepiride to improve pancreatic beta-cells in T2DM patients. Beta cells are a type of cell found in the pancreatic islet and also synthesize, secrete insulin and amylin. Sitagliptin is found to be maintaining the beta-cells function and glycemic control in T2DM patients. Randomly diabetes mellitus type-2 (T2DM) patients’ data is collected (110 patients) for statistical analysis. For this study, T2DM patients enrolled with the age of 30-60 years, treated with Sitagliptin and Glimepiride once a day. The baseline of HbA1c >7.0% to <10.5%; thus, the performed student t-test is to find out the significant role. The 110 patients are distributed into two groups: one Sitagliptin user group (n=62) and the second Glimepiride user group (n=43). The sitagliptin treatments may also protect also beta cells as the pancreas may not able to regenerate...
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