Posted by admin on Oct 31, 2021 in |
Two monobasic bidentate ligands L1H [2-(5-Bromo-2-dihydro-2-oxo-1H-indol-3-ylidene)hydrazine-carboxamide] and L2H [2-(5-Bromo-2-dihydro-2-oxo-1H-indol-3-ylidene)hydrazinecarbothioamide] were synthesized by the condensation of [2-(5-Bromo-2-dihydro-2-oxo-1H-indol-3-ylidene) with semicarbazide and thiosemcarbazide, which formed a series of complexes with cobalt(II) and nickel(II). The ligands and the metal complexes were characterized based on elemental analysis, IR, UV–Visible, 1HNMR, Mass spectroscopy. Spectral studies revealed that the ligands acted as monobasic bidentate, coordinating to the metal ion through the azomethine nitrogen i.e. >C=N and carbonyl oxygen atom of the isatin moiety. All the Schiff base metal complexes have a mononuclear structure, and the octahedral geometry has been proposed on the basis of spectral studies. The nickel (II) complex Ni (L2) (OAc).3H2O was subjected to XRD studies which revealed a monoclinic crystal system. In vitro, biological screening effects of the compounds were tested against two bacterial species Staphylococcus aureus and Escherichia coli. DNA cleavage and antioxidant activity of the ligands and their respective complexes were also...
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Posted by admin on Oct 31, 2021 in |
Datura (Datura metel Linn.) is a herbaceous leafy annual and short-lived plant of the Solanaceae family. It’s commonly known as Angel’s trumpet, Devils apple, Jimson weed, stinkweed, and Thorn apple. The traditional system of medicine enlightened the importance of Datura metel Linn. of its great medicinal value. The roots, leaves and flowering tops have been used in India for their narcotic and antispasmodic properties in the treatment of numerous ailments and conditions. The juice of fresh leaves is used to relieve the painful swellings. The seeds and root possess anti-diarrheal and are used to treat insanity and fever. All parts of Datura are poisonous in nature, and it’s mentioned under sthavara visha category by Ayurveda. In the present study, roots of Datura, which are less toxic compared to the other parts of the plant, were studied both in raw and purified form. AS the drug, in general, was considered poisonous, purification was done with Ayurveda procedure (Shodhana Samskara) to reduce the toxicity of the noxious drug for its chemical...
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Posted by admin on Oct 31, 2021 in |
This study is focused on evaluating the detrimental factors of drinking water and its relation to blood glucose level and anxiety status of non-stressed experimental rats. The study was conducted on 30 adult Wistar albino rats. The control group was treated with filtered water by reverse osmosis unit, and test 1, test 2, test 3, and test 4 groups were treated with well water from rural area 1, rural area 2, urban area 1, and pipeline water from urban area 2 respectively. Each week, body weight and fasting blood glucose (FBG) levels were measured. From the 11th week onwards, animals’ anxiety response was measured using an elevated plus maze (EPM). On the 80th day of the experiment, all the animals were sacrificed, and blood was collected, and the pancreas was removed for further investigations. On 9th week of the experiment, FBG level became 83 ± 3.89 mg/dL, 94.6 ± 10.05 mg/dL, 79.8 ± 2.54 mg/dL, 79.5 ± 4.51 mg/dL, 107 ± 6.14 mg/dL for control, test 1, test 2,...
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Posted by admin on Oct 31, 2021 in |
Actino bacterium Streptomyces sp. NLKPB45, which was isolated from mangrove soil sediments of Nellore coastal regions of Andhra Pradesh, India, was used in shake-flask fermentation to explore the different carbon and nitrogen substrates for antimicrobial crude compound. Careful experimentation of ranging each variable in the fermentation medium found that glucose and peptone as preferred carbon and nitrogen sources at 1% (w/v) concentration each, at pH of 7 and 28 °C incubation temperature for 8 days of incubation time. Response surface methodology-based statistical optimization of the process variables with their interconnection was analyzed using a central composite design. A second-order quadratic polynomial equation yielded a complacent fit for experimental with respect to crude compound concentration. Analysis of variance statistics showed that significant-high R2 value for model and adjusted and predicted; R2 values were also shown decent agreement with observed and predicted results. The results have confirmed very good interaction among five process variables at optimized values of 7.51318 pH, 28.734 °C incubation temperature, 8.18409 days incubation time, 1.05185 g/L glucose...
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Posted by admin on Oct 31, 2021 in |
Objectives: The present study aimed to develop simple and accurate first and second-order derivative spectrophotometric methods (1D and 2D) for the assay and stability studies of Ranolazine (RAN) in bulk and dosage forms. Methods: The original UV spectrum of RAN solution in methanol was differentiated instrumentally to generate the first and second derivative spectra which were measured at 278 nm and 283 nm, respectively. The developed methods were then validated as per ICH guidelines and applied for the stability studies. Results: Beer’s law was found to be valid over the concentration range 100-600µg/ml with a correlation coefficient (r) not less than 0.999. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 24µg/ ml and 73 µg/ ml, 17.8 µg/ ml and 53.6 µg/ ml, for 1D and 2D respectively. Good results were also obtained from the assay of RAN tablets (98.6 ± 2.3%, n=3). Studying the stability behavior of RAN using the developed methods reflects its instability under alkaline conditions following the first-order kinetics....
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