Posted by admin on Sep 30, 2021 in |
The present study investigates the isolation and identification of Multi-Drug Resistant bacteria from Hospital Effluent and the determination of Antimicrobial activity of Terminalia bellerica (Roxb.) against the isolated Multi Drug-Resistant Bacteria. Hospital effluent was collected and screened for the presence of Drug resistance phenotype against five different antibiotics, and the isolated bacterium was identified by 16s rRNA sequencing. Terminalia bellerica Roxb., Baheda has established antimicrobial activity against Gram-negative and Gram-positive bacteria hence the above plant was selected for the present investigation. Ethanolic extract of Terminalia bellerica outer coat, fruit, and the seed was tested for antimicrobial activity against the isolated Multi Drug-Resistant Bacteria, and the MIC90 value was determined. Ethanolic extract of T. bellerica outer coat having the highest zone of inhibition was further fractionated, and four individual fractions, F1 (water), F2 (50% ethanol and water), F3 (ethanol), and F4 (acetone) were tested for antimicrobial activity against isolated Multi Drug-Resistant Bacteria. Staphylococcus saprophyticus, which was isolated from Hospital effluent and identified by 16 s-rRNA sequencing, was found to be...
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Posted by admin on Sep 30, 2021 in |
Pioglitazone is an oral anti-diabetic agent used in treating class – II diabetes mellitus. T1/2of pioglitazone was 3-6 hrs and is eliminated rapidly. Hence sustained release is needed to prolong its duration of action and to increase its oral bioavailability. The main aim of the study was formulation and evaluation of an in-situ gel system of Pioglitazone to increase its bioavailability as a convenient dosage form. Method of Ion-sensitive in-situ gelation was used in this study. Total 15 formulations were prepared with Guar gum, Xanthan gum, and Carbopol-934 in various combinations and assessed for physical appearance, pH, viscosity, in-vitro gelling capacity, drug content, and in-vitro drug release. FTIR, DSC for Pioglitazone, excipients used, and optimized formulation were conducted. In-vivo drug kinetic studies were conducted for optimized formulation. Formulations showed an optimum viscosity allowing ease of administration and swallowing. All formulations have shown pH between 6.9-7.3, floating lag time was 2-3sec and floated for >12 hrs. The x-ray image studies are also confirming the same thing. In- vitro drug release...
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Posted by admin on Sep 30, 2021 in |
The current research paper describes a highly specific, reproducible, and efficient stability-indicating HPTLC method for estimation of Celecoxib and Amlodipine Besylate from its synthetic mixture. Chromatographic separation and quantification carried out on Merck HPTLC aluminum sheets of silica gel 60 F254 using Chloroform: Acetone: Toluene: Methanol in the ratio of 5:3:2:0.5 v/v/v/v as solvent system. This system was found to give compact and dense spots for CXB and AML with the Rf value of 0.80, 0.23 respectively. Densitometric analysis of CXB and AML was done at 238 nm and 366 nm. Regression analysis for the calibration plots was indicative of good linearity between response and concentration over the range of 0.2-1.2 µg/spot for AML and 4-24 µg/spot for CXB. Forced degradation studies were performed under different conditions. Both drugs were degraded in acidic, basic, oxidative, thermal, and photolytic conditions. In the present research, a stability-indicating HPTLC method has been developed for Celecoxib and Amlodipine Besylate. The developed method was validated as per ICHQ2R1 guidelines and was successfully applied for...
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Posted by admin on Sep 30, 2021 in |
The present study of concurrent process validation delivers an extraordinary degree of quality assurance that a specific process for manufacturing of Glibenclamide Tablets will consistently manufacture a product that meets its predetermined quality attributes and specifications. Glibenclamide is generally suggested for the treatment of type II diabetes mellitus and it is mainly a sulfonylurea derivative. It mainly comprises the stages to be followed to evaluate and qualify the acceptability of the manufacturing process of Glibenclamide 2.5 mg tablets. The process is limited to the three batches H, I, and J manufactured of specific batch size with the help of specified equipment’s and different quality control parameters for tablets. It involves all parameters related to each step were evaluated by the respective standard test involved in the manufacturing. All analytical results of each stage were found to be within the acceptable limit and criteria. Other tests related to compression such as hardness, thickness, disintegration and dissolution for all three batches were also found within the acceptable...
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Posted by admin on Sep 30, 2021 in |
Piperazine is a nitrogen-containing heterocyclic compound and has fascinating applications in drug discovery and development. In the present study, novel 4-(benzo[1,3]dioxol-5-ylmethyl) piperazine amide derivative was prepared by acid amine coupling of 1-piperonyl piperazine derivative and benzoic acid. Synthesized compound was characterized by IR, NMR and mass spectral studies. Further, in-vitro cytotoxic effects were carried out in MTT in various human cell lines HeLa, MCF7, MDA-MB-231, HCT116, and HT29. Out of these diverse cell lines, compound 3 showed promising cytotoxic effects on MDA-MB-231 with the IC50 estimation of 11.3 µM. The surface morphology by Colonogenic assay and in-vitro cell migration assay measures better movement on MDA-MB-231 in compound 3. Further, apoptotic-related examination under AO/Eb staining, commet assay, and cell cycle investigation exhibited that compound 3 demonstrated significant activities on MDA-MB-231 by activates apoptosis and blocking cell cycle moment in the G0/G1 stage. All the more altogether, staining the cells in the meantime with Annexin V-FITC/PI. The synthesized compound 3 indicated tremens-dous repressing capacities in cell cycle and activates apoptosis because...
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