Posted by admin on May 31, 2025 in |
Objective: The present investigation demonstrates a simple, sensitive and accurate high-pressure liquid chromatographic (HPLC) method for the determination of Alvimopan (AMP) in bulk and dosage form. The chromatographic separation was achieved within 10 minutes by using acetonitrile: potassium dihydrogen phosphate buffer pH 3.0 adjusted with orthophosphoric acid (50:50) as mobile phase on Altima Grace Smart C-18 column (5μ; 250 × 4.6 mm) at flow rate of 1.0 ml/min with injection volume 20µL at wavelength of 261nm. The retention time of the drug was found to be 5.02 min. This method was validated as per United States Food & Drug Administration (US-FDA) guidelines. The results of the validation parameters were found to be within the acceptance limits. The method was linear in the concentration range from 500-20000 ng/mL (r2 = 0.9998) and the accuracy of drug was found to be 95.83-104.63% for AMP. The lower limit of quantification was found to be ng/ml and the stability of recovered samples at different conditions was found to be more than 95%. The...
Read More
Posted by admin on May 31, 2025 in |
Background: Breast cancer is the most occurring cancer in women and the second most reason for tumour related deaths in women. Chemoprevention, which involves employing dietary, natural, or synthetic items, has emerged as an intriguing strategy to deal with increase in Breast cancer cases. Plumeria pudica Jacq. is fast growing, having medium size and belongs to the family Apocynaceae. The high concentration of phenols and phenolic chemicals in P. Pudica suggests that it may be utilised to cure cancer. Materials and Methods: In present study, mammary carcinoma was induced in female wistar rats by intragastric (IG) administration of 7, 12-dimethylbenz[a] anthracene (DMBA) at a dose of 80mg/kg of body weight. Treatment of methanolic extract of plumeria pudica leaves (200mg/kg/BW, thrice a week) to breast tumor bearing rats was found to be effective against DMBA induced mammary carcinoma. Progression of mammary carcinoma associated with decrease in body weight, anaemia, leucocytopenia, elevated levels of tumorigenicity, increase in oxidative stress and increased Liver biomarker levels. Result: The study showed the ability of...
Read More
Posted by admin on May 31, 2025 in |
The study provides a detailed morpho-anatomical description of Tonna dolium (Linnaeus, 1758), a commercially important tonnid gastropod, based on specimens collected from the Gulf of Mannar, India. The research examines the shell characteristics, head-foot morphology, mantle organs, visceral mass, reno-pericardial system, digestive system, reproductive system, and central nervous system of T. dolium through dissections and histological analysis. Key anatomical features are described and illustrated, including the proboscis structure, radula morphology, esophageal glands, and genital systems of both males and females. Comparisons are made with previously studied tonnid species, particularly Tonna galea and Tonna pennata. Notable distinctive features of T. dolium include the narrow gill and osphradium, radularrachidian with two pairs of secondary cusps, and confinement of seminal vesicles within the pallial oviduct. This comprehensive anatomical study contributes to a better understanding of tonnid taxonomy and provides insights into the adaptations of T. dolium for its predatory lifestyle in tropical marine...
Read More
Posted by admin on May 31, 2025 in |
The current study aims to find out how effective different extractions of Barleria cristata leaves are at fighting cancer, free radicals, and bacteria. Physical and chemical qualities and preliminary phytochemical analysis will also be assessed. It also quantifies several active ingredients by total phenolic and flavonoid. Five leaf extracts of Barleria cristata were made with ethyl acetate, chloroform, acetone, ethanol, and water. They were then tested for their antioxidant potential using reducing power and free radical scavenging methods, such as DPPH and NO radicals. They were also tested for antibacterial activity against Gram-positive and Gram-negative bacteria. The MTT assay measured how successfully the medication killed MCF-7 breast cancer cell line and Hep-G2 hepatoma cancer cell line. We calculated IC50. Initial phytochemical analysis was performed on all extracts. The total extract was abundant in protein, carbs, tannins, flavonoids, and phenolics. Acetone extract had higher phenolic and flavonoid components than ethyl acetate, chloroform, ethanol, and water. Barleria cristata acetone extract was antioxidant rich. Plant extracts also had antibacterial properties. Extracts inhibited...
Read More
Posted by admin on May 31, 2025 in |
Objective: Melasma is a chronic acquired hyper melanosis of skin, involving sun exposure areas of the face and neck. Even with various treatment modalities, melasma recurs and long-term treatment is associated with many side effects. Tranexamic acid, the antifibrinolytic agent is a drug of research for melasma, as it inhibits melanogenesis. Hence, the present study compares the efficacy and safety of oral Tranexamic acid (TXA) with topical Triple Combination Cream (TCC) containing fluticasone propionate 0.05%, tretinoin 0.025% and hydroquinone 2% versus topical triple combination alone in the treatment of melasma. Methods: About 120patients who met the inclusion criteria were randomly assigned into two groups of 60 patients each. Group A was treated with TCC at night and group B received oral TXA 500mg once daily morning and TCC at night for 8weeks. All patients were advised to apply sunscreen with SPF 50. The follow-up was done in the 4th and 8th week and MASI score (efficacy) and side effects if any, like deranged clotting time, bleeding time and ophthalmology...
Read More
