Posted by admin on Jul 31, 2021 in |
The present study was carried out to investigate the phytochemicals, Total polyphenolic content and In-vitro antioxidant activity of ethanolic and aqueous extracts of Cordia obliqua fruits. Objective: To determine the primary and secondary metabolites present in different extracts, to aces the quantity of phenolic compounds by total phenolic content and to evaluate the % inhibition of free radicals by in vitro antioxidant activity of Cordia obliqua fruits Methods: The plant material was extracted with solvents with increasing order of polarity and phytochemicals were identified by different chemical tests. Preliminary phytochemical studies of fruit extracts of Cordia obliqua revealed the presence of phytoconstituents like glycosides, alkaloids, tannins, phenols, saponins, in the extracts. The different concentration of extracts and standard ascorbic was used to identify the scavenging effect by in-vitro antioxidant models. Results: Phytochemical analysis of extracts revealed the presence of glycosides, alkaloids, tannins, phenols, saponins. The phenolic content in different extracts was found to be 150.16, 174, and 231.39, respectively. The results of in-vitro antioxidant tests suggested that the ethanolic...
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Posted by admin on Jul 31, 2021 in |
Distinctive groups of fungi are involved in the symbiotic association with epiphytic orchid. We have isolated endophytic fungi from Cleisostoma tenuifolium epiphytic orchid found in the Western Ghats and used morphological criteria as well as molecular techniques to identify these fungi. Characterization of these fungi was done by analyzing the quantified genomic sequences, and then identification was done by comparing the ITS region sequence of obtained fungi with its closely related fungal species from the NCBI database. Molecular techniques were useful in identifying endophytes to its species level. The vast range of endophytes from a single type of orchid further highlights the importance of forest epiphytes as reservoirs of fungal biodiversity; Schizophyllum commune, Stagonosporopsis cucurbitacearum, Xylaria adscendens, Fusarium...
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Posted by admin on Jul 31, 2021 in |
Introduction: Global pneumonia mortality (50%) caused by pneumococcus is responsible for invasive pneumococcal disease. Pneumococcal Conjugate Vaccine (PCV) has been introduced by the Government of India in a phased manner in selected states, but Haryana was not included. Objective: To draw key lessons learned from the process of newer vaccine introduction (PCV) in Haryana from the state’s resources to inform future introductions of PCV and any other new vaccine in other states of India and internationally. Methods The process of PCV introduction in Haryana was introduced with the launch of “Atal Jeevan Rakshak Teekakaran Yojna,” in 22 districts of the Haryana. The highest political and administrative leadership with a strong commitment to reducing the IMR and U5MR, approved the proposal and budget (~12 million USD). It was planned use of the of PCV13, and follow the National Immunization schedule i.e. 2 primary and 1 booster dose at 6, 14 weeks, and 9 months. The State-level launch ceremony of PCV introduction was organized in November 2018. Findings: Haryana became the...
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Posted by admin on Jul 31, 2021 in |
Colon carcinogenesis is a multistep process that arises by the accretion of genetic alterations. The genetic and epigenetic changes which transform normal colonic epithelium into aberrant crypt foci which is responsible for penetration. The GC-MS analysis of ethanolic extract of Epiphyllum oxypetalum (DC.) have shown the presence of phenolic compound (4-hydroxy-2-methylacetophenone), Diterpene, steroids which were reported for antioxidant and anticancer activity. In the present study Brine shrimp lethality, Trypan blue assay, and MTT assay in-vitro models are performed. In an in-vivo study, animals were divided into 5 groups as Normal, Control (NMU + distilled water 10ml/kg.), Standard (5-Flurouracil with the dose of 10mg/kg via intraperitoneal route given once in a 3 days of interval for 15 days to complete 5 cycles.), Test I (100mg/kg absolute ethanol extract of leaves of plant Epiphyllum oxypetalum (DC.)) and Test II (200mg/kg absolute ethanol extract of leaves of plant Epiphyllum oxypetalum (DC.)) groups each group comprised 6 animals. Cancer were induced in rats by using N-Methylnitrosourea by intra-rectal instillation method in a dose of 0.4% solution...
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Posted by admin on Jul 31, 2021 in |
Background: This was a conventional comparative treatment-controlled study designed to analyze the effect of dopamine infusion in the treatment of prerenal azotemia (PRA). Material and Methods: thirty randomized consecutive adult patients (group A= 15, B=15) aged 18 and 58 years with PRA were recruited. Group a received conventional treatment only (fluid replacement and frusemide), while group B received conventional treatment and dopamine. 24 hours urine volume, hemodynamic changes (pulse and blood pressure), serum electrolyte, urea, and creatinine were measured for the first 5 days of hospitalization. Duration of oliguria and hospitalization, mortality, number of patients who developed acute tubular necrosis (ATN), and or required dialysis was computed. Results: Duration of oliguria and hospitalization were shorter in group B than in group A patients (P = 0.025). The daily and hourly urine output was more in group B than in group A patients (P = 0.025). However, from the second day, patients in group B excreted more sodium than patients in group A (P = 0.001). 5 patients in group...
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