Posted by admin on Apr 30, 2021 in |
Forced degradation studies are a vital tool in pharmaceutical research and development to predict long-term stability. Stress studies should be performed in method development to know drug behavior but can also be performed with method validation for regulatory filling predict stability, and measure impurities. It is especially valuable when little data is free about potential degradation products. Forced degradation studies may help encourage drug advancement in regions like formulation development, manufacturing, and packaging, in which information on substance conduct can be utilized to improve a medication item. Hence it is important to realize the purity profile and conduct of a medication substance under various natural conditions. For stable formulation development, comprehension of synthetic conduct forced degradation pathways and degradants of drug substance and drug product is very important. Therefore, by using different regulatory guidelines in the present review paper, we described the broad overview of forced degradation studies by determining the system to perform stress studies & its methods for isolation and identification of...
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Posted by admin on Apr 30, 2021 in |
To investigate the phytoconstituents and anticancer potential followed by apoptotic studies of H. undatus fruit extracts on HepG-2 cells. Qualitative phytochemical profiling and anti-proliferation activity of Aqueous, Chloroform, Ethyl acetate, Hexane, and Methanol extracts of dragon fruit pulp was carried out. Based on the results of anti-proliferation studies, methanol extract was taken further for apoptotic study by treating HepG-2 cells for nuclear staining using DAPI and GC-MS analysis to elucidate the presence of active compounds. Qualitative phytochemical analysis revealed the presence of carbohydrates, tannins, saponins, anthocyanin, quinones, cardiac glycosides, terpenoids, triterpenoids, phenols, acids, and steroids in aqueous fruit extract. Similarly, chloroform fruit extract showed the presence of carbohydrates, saponins, alkaloids, cardiac glycosides, triterpenoids, phenols, and coumarins. Likewise, Ethyl acetate fruit extract had carbohydrates, tannins, saponins, flavonoids, alkaloids, anthocyanin, cardiac glycosides, terpenoids, triterpenoids, acids, and steroids. In hexane fruit extract, carbohydrate, saponins, anthocyanin, quinones, phenols, and acids were present. But more phytocompouds such as carbohydrates, tannins, saponins, flavonoids, alkaloids, anthocyanin, cardiac glycosides, terpenoids, triterpenoids, phenols, acids, and steroids were found...
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Posted by admin on Apr 30, 2021 in |
Quercetin (QCN) is a flavonoid available in fruits and vegetables, and it has shown several medicinal properties, including anticancer properties. However, the radiosensitizing properties of QCN on adenocarcinoma cells have not been greatly looked into yet. Thus in the present study, we investigate the effect QCN would have on the lung adenocarcinoma cell line NCI-H23 (H23) when QCN is combined with radiation. We checked for QCN’s effect on cell survival of H23 and the embryonic HEK293T cells. We checked the effect that QCN alone and QCN in combination with radiation would have on oxidative stress, metastatic proteins MMP-2 and p21, the expression of AKT1 and PTEN genes, and the apoptosis-associated genes in H23 cells. We observed that QCN alone and in combination with radiation lowered the cell survival of H23 cells while not affecting the cell survival of HEK293T cells. The QCN treated groups showed a spike in oxidative stress, lowering of the levels of MMP-2 and p21 proteins, up-regulation of PTEN gene and down-regulation of AKT1 gene expression,...
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Posted by admin on Apr 30, 2021 in |
Malnutrition is a condition in which there is an imbalance in the proportion of essential nutrients that the body requires to stay healthy. The most prevalent form of malnutrition is micronutrient deficiency and of this iron deficiency anaemia is the most common type, affecting over 2 billion people worldwide. Micronutrients are a special class of vital ingredients that are required in minuscule quantities to perform a wide range of physiological functions. The current therapy for the treatment of Iron deficiency anaemia has many drawbacks like gastrointestinal side effects, erratic absorption of iron, etc. To overcome the above side effects, we tried to develop and evaluate a site-specific sustained-release combined drug delivery system of Iron and Folic acid. Iron as Ferrous ions is preferentially absorbed in the jejunum. To facilitate the absorption of iron, the gastro retentive multiunit particulate system (GRMUPs) was developed comprising Ferrous Ascorbate is a source of iron. Colon targeted tablet was developed for Folic acid to localize and maximize the absorption of Folic acid in the...
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Posted by admin on Apr 30, 2021 in |
Preliminary screening study had employed to extract inhibitors of calcium oxalate (CaOx) crystallization in-vitro from four plant species, namely Saraca asoca, Berginia ciliata, Boerhavia diffusa, and Crateva nurvala These plants were selected based on their frequent medicinal usage by folks. Plant material (150 g) used for this study was successively extracted with Soxhlet apparatus using the solvents in order of increasing polarity (ethyl acetate, acetone, ethanol and water). Each time the marc was dried and later extracted with other solvents. All the extracts were concentrated by distilling the solvent in a rotary vacuum evaporator. An extracts were prepared at different concentration (1 mg-10 mg/ml). Nucleation assay and aggregation assay of calcium oxalate crystals were measured using spectrophotometric methods. The plant extracts promoted the precipitation of calcium oxalate particles in buffer and inhibited calcium oxalate crystal aggregation which clearly indicates the antilithiatic activity of these plants. Phytocompounds present in this plant may be responsible for the anticrystallization activity. Statistical difference and percentage inhibitions were calculated and assessed. The results were...
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