Posted by admin on Apr 30, 2021 in |
Deoxycholic acid (DCA) is a secondary bile salt that is a major active constituent of Chinese traditional medicine, “Niuhuang” which is known to have immunoregulatory and anti-inflammatory properties. Although data from the literature indicate possible immunomodulatory effects of Deoxycholic acid on the immune system, little has been reported regarding the mode of activity of DCA in modulating the immune system. The objective in the present study was to determine the effect of Deoxycholic acid (DCA) on immune parameters in mice and rats. The current study employed both in-vitro and in-vivo protocols in mice and rats. In-vitro study results indicate that DCA causes splenocyte proliferation at lower doses but is cytotoxic to splenocytes at higher doses were observed in these supernatants that were sensitized with DCA versus splenocytes derived from control animals. Significant differences between normal and DCA treated animals were observed in the following parameters: white blood cell indices, absolute weights of lymphoid organs, and spleen cell proliferation to DCA stimulation. These results indicate that DCA treatment modulates the...
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Posted by admin on Apr 30, 2021 in |
Levoleucovorin calcium contains amide and aldehyde groups which makes it vulnerable to degradation. Stability indicating method for determination of all seven process-related impurities and degradation impurities in Fusilev® 50 mg/mL injection was developed with the help of RP-HPLC. A Forced degradation study supports method development to ensure stability indicating conditions. Solutions of the lyophilized injection were stress-tested in different conditions like Acidic, Basic, Oxidative, Thermal, Photolytic conditions, and the mass balance was found close to 90-110%. Validation has been performed according to ICH guidelines. The method was developed in ACE C18 column (150 × 4.6 mm, 3 µm) with binary gradient elution consisting of mobile phase A, which consists a mixture of Potassium Phosphate Triabasic and Tetra butyl ammonium hydrogen sulfate in water (pH 6.8 with diluted Ortho Phosphoric Acid, 0.05 M, 1000 ml) and mixed with Methanol in ratio of 90:10 % V/V and mobile phase B consist methanol at a flow rate of 0.5 mL/min and detection performed at 280 nm with column thermostat 40 °C, injection...
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Posted by admin on Apr 30, 2021 in |
The main aim of the present study was to formulate and evaluate valacyclovir-loaded liposomes. A drug and excipient compatibility study was performed by FT-IR, and the study revealed that there was no interaction between drug and excipients. Liposomes are prepared by using two different methods, i.e., ether injection method and thin-film hydration method. Various formulations are prepared by varying the concentration of phospholipid and cholesterol. All formulations are evaluated for entrapment efficiency and in-vitro drug release studies. The optimized formulation showed the highest entrapment efficiency (96.51%) and highest drug release (84.21%). The optimized formulation was evaluated for FT-IR, SEM and particle size analysis, and zeta potential studies. The FT-IR study revealed that there is no interaction between drugs and excipients. The optimized formulation was found to be stable with zeta potential -40.9 mV and particle size of 67.4 nm with uniform distribution. The regression coefficient (R2 value) 0.966 indicating release as zero-order, where “n” value 1.212 states the mechanism as a super case-II transport mechanism. Stability studies were carried...
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Posted by admin on Apr 30, 2021 in |
The present study evaluates the antihyperglycaemic and antioxidant activity of the methanolic extract of Capparis decidua (CDMtE) fruits in streptozotocin (STZ) induced diabetic rats. Streptozotocin (50mg/kg body weight/) was injected intraperitoneally in rats to induce diabetes. The STZ-induced diabetic rats were treated with CDMtE (200 mg/kg body weight /day) for seven days. Biochemical analysis, toxicity studies, and histopathological studies were carried out to assess the antihyperglycaemic and antioxidant effects. Treatment with CDMtE (200 mg.kg b.wt/day) caused a significant decrease in fasting blood glucose and improvement in the serum insulin level of the diabetic rats. Antioxidant enzymes SOD and GSH activity was increased, and LPO levels of diabetic rats decreased after oral administration of CDMtE (200 mg/kg body weight/day). Histopathological studies showed significant recovery in liver architecture after CDMtE administration. Histopathological results suggest the antihyperglycaemic and antioxidant ability of CDMtE. This study supports the therapeutic potential of C.decidua fruits in the treatment of...
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Posted by admin on Apr 30, 2021 in |
Medicinal plants are considered to be the most important in the primary health care system of developing countries. The therapeutic properties of a plant depend upon the biochemical compounds present in the plant parts employed in the preparation of a medicine. Trichosanthes dioica Roxb. is being used as popular Ayurveda and Siddha medicine used to treat various diseases. Objective: The objective of this research was to carry out for identification of bioactive compounds from ethanolic extracts of leaf and fruit of Trichosanthes dioica Roxb. by Gas chromatography and Mass spectroscopy (GC-MS). Methods: The air-dried leaves and fruits were powdered and separately extracted with ethanol by using a Soxhlet extractor; then, each of the extracts was subjected to qualitative phytochemical analysis and further subjected to a GC-MS instrument. Results: The result of the qualitative analysis of plant extracts has a wide range of active phytocompounds such as alkaloids, flavonoids, glycosides, phenols, saponins, steroids, reducing sugar, tannins, and terpenoids. The GC-MS analysis, twenty two different phytochemical compounds were identified in T....
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