Posted by admin on Mar 31, 2021 in |
Introduction: Most potent toxic elements are derived from nature only. So safety and toxicological evaluation of plant extracts which are otherwise thought to be very safe, are mandatory. The zebrafish embryo has become an important model organism for toxicological research due to its small size, genetic similarity to humans, etc. The Objective: The objective of the present study is to determine acute toxicity that is lethal concentration fifty (LC50) of whole plant ethanolic extract of Amaranthus viridis (A1), Aerva sanguinolenta (A2) & Cynodon dactylon (A3) on Zebrafish (Danio rerio) embryos at a different time interval. Material and Method: Acute toxicity testing of the three whole plant ethanolic extracts A1, A2, A3 with six different concentrations each were performed according to OECD guideline 236 on Zebrafish embryos. Embryo development was monitored after 24 h interval for 120 h. After incubation, the living and dead embryos were evaluated using Light Microscope and images obtained with an optical camera. Statistical Analysis: LC50 values were calculated using the Probit method in the SPSS...
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Posted by admin on Mar 31, 2021 in |
Aims & Objective: To monitor therapeutic drug level of serum phenytoin for early posttraumatic seizure prophylaxis in traumatic brain injury (TBI) patients using enzyme multiplied immunoassay technique (EMIT) in a tertiary care hospital. Materials and Method: In this observational, open-label, non-interventional, prospective study, 90 patients of mild to moderate TBI patients were recruited, and serial monitoring of total serum phenytoin level along with concomitant serum albumin was done on day 3, 5, and 7 using EMIT assay. The monitoring of phenytoin drug levels along with Glasgow coma score and any episode of posttraumatic seizures was assessed. Results: The most common mode of injury was road traffic accidents (61.1%). Patients with total serum phenytoin level in sub-therapeutic level on days 3, 5, and 7 were 20%, 15.6 %, 14%, respectively. The toxic range % was 0%, 1.1%, and 7% on the three respective days. Conclusion: This observational study concludes that serial therapeutic drug monitoring (TDM) of phenytoin in TBI patients has the advantage of optimizing the drug level within the...
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Posted by admin on Mar 31, 2021 in |
Currently, pharmacological activities of Eclipta alba (L.) plant extracts and individual phytoconstituents have revealed anticancer, hepatoprotective, snake venom neutralizing, anti-inflammatory and antimicrobial properties. The role of antioxidants is increasing day by day due to their multiple roles to reduce the harmful effects of oxidative stress. Phytoconstituents like wedelolactone and ursolic and oleanolic acids as well as luteolin and apigenin can form the basis of new drugs against cancer, arthritis, gastrointestinal disorders, skin diseases, and liver disorders. Plants have all these activities due to biologically active compounds, and for this, we have analyzed phytochemical screenings and antioxidant activity through the DPPH, ABTS●+, and reducing power assay and we got maximally phenols then flavonoids and flavonols and a good natural antioxidant agent. The best-known compound in E. alba i.e., Wedelolactone. It was analyzed by TLC, and it was present as 0.52 Rf value and present in pet ether extract and acetone extract and minimum in ethanol extract. The antioxidant activity was assessed through DPPH, ABTS●+ free radical scavenging activity and reducing...
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Posted by admin on Feb 28, 2021 in |
In the synthesis of Nebivolol [a mixture of (1RS, 1′ RS)-1,1′-{(2RS, 2′ SR)-bis(6-fluoro 3, 4-dihydro-2H-1-benzopyran-2-yl)} -2,2′-iminodiethanol] having four chiral centers, one crucial isomeric impurity at 0.88 RRT was observed in HPLC related substance analysis in gradient HPLC method. This impurity was isolated from synthesized crude impurity substance by preparative HPLC with chiral normal phase and was elucidated as [(R)-1-[ (R)-6-Fluorochroman -2-yl]-2-({(R)-2- [(R)-6-Fluorochro man-2-yl] -2-hydroxyethyl} amino) ethanol] (R, R, R, R- Nebivolol) by means of chromatographic and spectral data. Structural elucidation carried out by spectral data was...
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Posted by admin on Feb 28, 2021 in |
Antibiotic resistance emergence, as well as the Darwinism of up to the minute strains of ailment generating agents, is of grave concern to the global health community. Sustainable therapeutics of an ailment entails the amalgamation of contemporary pharmaceuticals or some potential prospective of novel drugs. Customarily employed medicative plants of Gopalganj community could be an enticing of drugs to fight this problem. This study is targeted at a reconnaissance of the antimicrobial properties of the plants that are customarily being used as traditional health implementation. The antimicrobial potential of five different plant extracts was screened against three pathogenic microorganisms, which were isolated from typical non-healing wounds of patients of Gopalganj district hospital. The microorganisms chosen to evaluate for antimicrobial activity were Proteus mirabilis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. The favorable activity was shown by the extracts of Tridex procumbens L. was found to highest with zone of Inhibitions reaching to 18 mm, 19 mm and 20 they were nearly around the zone of inhibitions of positive...
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