Posted by admin on Feb 28, 2021 in |
The present study was aimed to formulate and evaluate floating microspheres of Baclofen. The research work’s objective was to retain Baclofen in the stomach for a prolonged period of time, which has absorption window in the upper gastrointestinal tract. The microspheres were prepared by solvent evaporation technique. A 32 full factorial was applied to investigate the combined effect of the two independent variables, i.e., the concentration of Eudragit RL100 (X1) and concentration of Eudragit RS 100 (X2) on the dependent variables particle size (Y1), percentage drug entrapment efficiency (Y2), percentage buoyancy (Y3), in-vitro drug release at 1 h (Y4), in-vitro drug release at 6 h (Y5). Results of the multiple regression analysis revealed that in-vitro drug release decreased and particle size, percentage drug entrapment efficiency, percentage buoyancy was increased with increasing the concentration of Eudragit RL100 and Eudragit RS100. The optimized formulation has a particle size of 115.96 µm, percentage drug entrapment of 90.06%, and buoyancy of 90.76%. In-vitro drug release of Baclofen floating microspheres showed a sustained release...
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Posted by admin on Feb 28, 2021 in |
The protein MTA1 with histone deacetylase activity is a member of the nucleosome remodeling complex encoded by the mta1 gene is frequently overexpressed in biologically aggressive epithelial neoplasms. MTA1 is a nuclear co-regulatory molecule that is highly expressed in many cancers and correlates with tumor metastasis and progression. MTA1 alters signaling events in the tumor micro-environment. MTA1 contributes to cancer progression, may serve as a biomarker and therapeutic target for cancer. The main objective of this study is to perform in–silico docking studies and energy minimizations for active sites of mutated MTA1 protein by selecting different naturally available antioxidant molecules in foods to facilitate ligand-binding site interactions and identify the best from the selected...
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Posted by admin on Feb 28, 2021 in |
Staphylococcus aureus is a frequent colonizer of the human body as well as a serious human pathogen. It can cope with stress factors and acquire resistance to antibiotics, thus rendering treatment difficult. Discovering new treatments for this disease is urgently required, especially in view of the emergence of multiple drug resistant organisms and to reduce the total duration of current treatments. Exfoliative toxin A (ETA), produced by Staphylococcus aureus causes scalded skin syndrome which is characterized by the separation of layers of skin. It affects newborns and adults primarily. Hence, therapies for Staphylococcal scalded skin syndrome (SSSS) are critically needed. Seeking a more effective compound for the treatment has been one of the great interests. In the present study, compounds from different plant extracts are permissible to interact with ETA using Schrodinger. The best effective compound gymnemic triacetate was selected from the list of different compounds based on its docking score and binding energy. The result showed that the herbal compound gymnemic triacetate possesses anti-bacterial activity by blocking exfoliative...
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Posted by admin on Feb 28, 2021 in |
Lannea coromandelica (Houtt.) Merr. which is known as Kayu Jawa in Bugis community, Indonesia has been widely used empirically for the treatment of wounds. Ethanol extract from its stem bark is known to have antibacterial, anti-inflammation, and antioxidant activity. The purpose of this study were to isolate, characterize, and determine the antioxidant and antimicrobial activity of the endophytic fungi from the roots of this plant. In this paper, we reported antioxidant and antimicrobial activity of the isolated endophytic fungi from Lannea coromandelica (Houtt.) Merr. and the isolation of the bioactive compound known as citrinin. The structure of citrinin was determined by extensive analysis of the spectroscopic data (1H-NMR, 13C-NMR, 2D NMR, and LC-MS) and comparison to those of the previous report. The antioxidant activity showed that the isolated compound has moderate activity with AAI value of 0.671 and IC50 145.9 ppm while the antimicrobial activity demonstrated that the isolated compound was not active against Escherichia coli ATCC 25922, Salmonella typhi ATCC 14028, Staphylococcus aureus ATCC 25923, and Pseudomonas aeruginosa...
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Posted by admin on Feb 28, 2021 in |
Objective: To investigate the bioactive constituents of Ulva lactuca, Sargassum prismaticum, and Sarconema filiforme using gas chromatography-mass spectrometry (GC-MS) analysis. Methods: 10 gm sample of each seaweed was successfully extracted with HPLC grade methanol and pet. ether with continuous shaking in a dark room for 36 h. The concentrated extracts were subjected to GC MS analysis. Results: The GC MS analysis of methanol and pet ether extracts of U. lactuca, S. prismaticum and S. filiforme show various types of phytocompounds screened under different R.T. Major compounds were 9-Octadecenoic acid (Z)-, phenyl-methyl ester, Stannane,1,3-dithian-2-ylidenebis[tributyl,Octadecane, 3-ethyl-5-(2-ethylbutyl) in methanol extract and Decane, 6-ethyl-2-methyl-, Tricosane and Sulfurous acid, 2-ethylhexyl hexyl ester in pet. ether. These bioactive compounds are considered as biologically and pharmacologically important. Conclusion: The three different extracts contain diverse bioactive compounds that were identified and characterized spectroscopically. Hence, the identification of different biologically active compounds in the extracts of seaweeds justifies further biological and pharmacological...
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