Posted by admin on Dec 31, 2020 in |
Ruthenium(II) complexes of the type [Ru(bpy) 2(L)](PF6)2, where L= 2-{4,5-bis[(E)-2-phenylethenyl]-1H-imidazol-2-yl}-1H-benzimidazole (1), 2-{4,5-bis[(E)-2-(4-chlorophenyl)ethenyl]-1H-imidazol-2-yl}-1H-benzimi-dazole (2), 2-{4,5-bis [(E)-2-(4-fluorophenyl) ethenyl]-1H-imidazol-2-yl} -1H-benzimidazole (3), 2-{4 ,5-bis[(E)-2- (4-methylphenyl) ethenyl] -1H-imidazol-2-yl }-1H-benzimidazole (4), 2-{4, 5-bis[(E)-2-(4-methoxyphenyl) ethenyl]-1H-imidazol-2-yl}-1H-benzimidazole (5) have been synthesized and characterized by elemental analyses and spectral (IR, UV-vis, NMR, ESI-MS) techniques. The redox behavior of the complexes has been studied by cyclic and differential pulse voltammetry. In acetonitrile solution, all the complexes exhibit characteristic metal to ligand charge transfer (MLCT) absorptions and ligand-based transitions. The complexes showed efficient DNA cleavage activity in the presence of light at the wavelength of 480 nm. The complexes are also able to cleave supercoiled pUC19 plasmid DNA via guanine base oxidation in a concentration-dependent manner. In-vitro cytotoxic activity of the complexes shows that complex 1 has better anticancer activity against MCF7 human breast cancer cells with an IC50 value of 7.9 µM. The antimicrobial activities of the ligand and their metal complexes were screened by agar diffusion method and found that the metal complexes have higher antimicrobial activity than the...
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Posted by admin on Dec 31, 2020 in |
The increasing public concern on the use of synthetic fungicide and resistance development led to an increase in search for alternative control strategies. Medicinal plants serve as therapeutic agents as well as important raw materials for the manufacture of traditional and modern medicine. Considering the vast potential of plants as sources for antimicrobial drugs with reference to antifungal agents, a systematic investigation was undertaken to explore the antifungal potential of few medicinal plants. In present study, in-vitro antifungal activities of few medicinal plant extracts were evaluated against two well-known plant pathogens i.e., Fusarium oxysporum (MTCC 284) and Cladosporium herbarum (MTCC 351). Fourteen plant ethanolic extracts were prepared, and out of them, five plants; Allium sativum, Zingiber officinale, Mentha spicata, Carica papaya and Elettaria cardamomum showed good antifungal activity. A. sativum ethanolic extract showing maximum antifungal activity was further subjected to carry out other studies. MIC values of ethanolic extract of A. sativum against F. oxysporum (MTCC 284) and C. herbarum (MTCC 351) were found to be 60 mg/ml and...
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Posted by admin on Dec 31, 2020 in |
A simple, reliable, and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the determination of lacosamide, an antiepileptic drug, in human plasma. The lacosamide and internal standard (IS) have been extracted by a simple liquid-liquid extraction method and separated on C18 (50 × 4.6 mm, 5μm) column with the mobile phase of 5mM ammonium formate and methanol (50:50, v/v), pumped at flow-rate of 0.5 mL/min. The ion transitions were monitored in positive ion mode [M+H]+ at m/z 251.20 >91.10 for lacosamide and m/z 256.10>211.10 for IS. Method validation revealed excellent linearity over the concentration range of 9 – 9000 ng/mL together with satisfactory intra and inter-assay precision, accuracy, and extraction recoveries. Lacosamide was found stable throughout the various sample handling and processing conditions. It is proposed to evaluate the performance of the developed method by measurement of a plasma concentration of lacosamide versus time in four epileptic patients to monitor pharmacokinetic behavior of it. The variation in estimated pharmacokinetic parameters shows the need for...
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Posted by admin on Dec 31, 2020 in |
Essential oils from plants have been reported to have antibacterial activity. Essential oil is a mixture of many chemicals, and one or more chemicals in essential oil may have antibacterial activity. In our laboratory, essential oil from the leaves of Ocimum sanctum L. was found to have antibacterial activity against 18 human bacteria. GC-MS analysis of the Ocimum sanctum essential oil revealed the presence of 19 chemicals, and one of them was eugenol. In the present study, eugenol was found to have an antibacterial effect against 4 Gram-negative and 2 Gram-positive human pathogenic bacteria by minimal bactericidal concentration, minimal inhibitory concentration and disc-diffusion methods. The minimal bactericidal concentrations of eugenol were 0.96 mg/ml, 4.17 mg/ml, 16.6 mg/ml, 16.6 mg/ml, 33.3 mg /ml and 33.3 mg /ml against Acinetobacter baumannii, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Serratia marcescens and MRSA, respectively. The minimal bactericidal concentrations and minimal inhibitory concentrations in Gram-negative bacteria Acinetobacter baumannii, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Serratia marcescens were similar. The minimal inhibitory concentrations are more than...
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Posted by admin on Dec 31, 2020 in |
Zingiber officinalis belonging to family Zingiberaceae is a plant of ancient cultivation that is traditionally used in several disorders. The principal components of ginger reported are gingerols, shogaols, and high content of starch (40 to 60 %). Not much work has been done on the separation of starch from ginger and its characterization. The main objective of this study was to investigate the potential of ginger starch to be used in Food, Pharma, as well as other industries. A simple, easy, convenient, and inexpensive method was designed to separate starch from ginger powder. The isolated ginger starch was then subjected to various physical and chemical characterization tests. The morphology of the starch granules (size and shape) was studied with scanning electron microscopy (SEM), which revealed oval and round forms of starch granules. Dynamic rheological properties of ginger starch were measured using Viscolead viscometer. The Molecular weight of starch was determined by Gel Permeation Chromatography (GPC) (1289858 g/mol). Ginger starch showed a gradual increase in swelling power and solubility index...
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