Posted by admin on Jan 31, 2021 in |
This present study aims to develop an accurate, precise, and linear reverse-phase High-Performance Liquid Chromatographic (RP-HPLC) method for the estimation of Pioglitazone HCL in Pharmaceutical dosage form. Method: The chromatographic system employs a reverse-phase Hypersil BDS, C8 250 × 4.6 mm, 5 columns using 0.01M Potassium phosphate buffer and acetonitrile (40:60) as mobile phase, methanol as a diluent in isocratic mode. A flow rate of 1.0 ml/min was optimized with a detection wavelength at 225 nm. The retention time (Rt) was around 4.72 ± 0.2 min. Results: The method was validated with respect to specificity, selectivity, linearity, accuracy, precision, and robustness as per ICH guidelines. The assay method was observed linear in the concentration range of 0.079-0.318 mg/ml with a Correlation coefficient (r2) of 0.9999. The percentage recovery of active pharmaceutical ingredients from tablet dosage form ranged from 99.40-100.40%. Stress conditions of degradation in acidic, alkaline, peroxide, thermal, and UV radiation were...
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Posted by admin on Jan 31, 2021 in |
Last few eras, the remarkable advancement in the drug delivery system has been done; the oral route remains the important and picks up the safest route of drug delivery. Regardless of outstanding advancements in the oral route medication, the current study focused on the formulation of rebamipide SR tablets by studying several blends and their characteristics, including drug-excipients compatibility studies. Ultimately, it was concluded that it is safe to use PEO N12K, Camphor (milled), and MCC in the formulation of the Rebamipide Sustained release tablet. Thus, rebamipide Sustained-release tablets are successfully prepared by using conventional wet granulation technique requires making a matrix type tablet and displaying drug-release through diffusion mechanism. This formulation will assuredly improve patient adherence, improve bioavailability, low side-effects, and sustained clinical activity over the required duration of time (24 h) after single-dose administration. For the determination of the drug content in formulation, a simple, rapid, accurate, robust, and specific UV-Spectroscopy method was developed for the assay of the rebamipide sustained release tablet. The detection was carried...
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Posted by admin on Jan 31, 2021 in |
IPB-3S Strain Oryza sativa bran, a high-antioxidant food, was expected to protect the brain as a susceptible organ from oxidative damage. This study was conducted to analyze the effect of rice bran extract on malondialdehyde (MDA) and glutathione peroxidase (GSH) levels in the brain induced by carbon tetrachloride (CCl4). A total of 24 Sprague-Dawley male rats was divided into 6 groups, including normal control (C1); negative control (C2); 150 mg/kg rice bran extract (I1); 150 mg/kg rice bran extract + CCl4 (I2); 300 mg/kg rice bran extract (I3); and 300 mg/kg rice bran extract + CCl4 (I4). The MDA levels were measured by Wills method, while GSH levels were measured by the Ellman method. The administration of low-dose (150 mg/kg) and high-dose (300 mg/kg) rice bran extracts significantly lowered MDA levels compared to control groups, both in the groups with and without the induction of CCl4 (p <0.05). Moreover, the administration of low-dose (150 mg/kg) also significantly increased GSH level (p <0.05), contrary to the groups administered by high-dose...
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Posted by admin on Jan 31, 2021 in |
Nanosuspension is a biphasic system consisting of drug particles dispersed in an aqueous surfactant or polymeric solution with a particle size between 10 to 1000 nm. Nanosuspension offers the unique advantage of increasing solubility of the pure drug resulting into faster drug absorption and hence achieving faster maximum plasma concentration. Hence, the aim of this research work was to formulate and characterize surfactants/polymer-stabilized nanosuspensions of diosmin (DSN), a phytomedicine, to surpass its poor physicochemical properties and low oral bioavailability. Nanosuspensions were prepared by probe sonication with varying concentrations of different surfactants and polymers such as PVP K 25, poloxamer 188, Tween 80, and PEG 400. Nanosuspension prepared with 3% w/v PEG 400 and 3% w/v DSN, exhibited the lowest size of 203.9 nm, PDI of 0.219, and adequate zeta potential of -27.26 mV, which was subjected to solubility and in-vitro drug release study. The highest solubilizing capacity of DSN was manifested in orthophosphate buffer pH 12. Approximately five times increase in saturation solubility of lyophilized nanosuspension was observed than...
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Posted by admin on Jan 31, 2021 in |
The present research work involves the use of exclusively and commercially available indole-3-carbaldehyde as a starting material to synthesize novel pyrimidine analogues. The synthesis of series of indole and pyrimidine containing N-substituted-4-(1H-indol-3-yl)-6-methyl-2-oxo-1, 2, 3, 4-tetrahydro-pyrimidine-5-carbohydrazide U(1-10) has been done by using principle of Biginelli condensation reaction.Indole-3-carbaldehyde was prepared by formylation of indole (Vilsmeier Haack reaction) using dimethylformamide and phosphorus oxychloride (1). The intermediate indole dihydropyrimidines was synthesized from a mixture of indole-3-carbaldehyde, urea and ethyl acetoacetate in the presence of zinc chloride as acid catalyst (2). The intermediate compound then refluxed with hydrazine hydrate by the addition of catalytic amount of conc. sulphuric acid, led to formation of parent compound 4-(1H-indol-3-yl)-6-methyl-2-oxo-1, 2, 3, 4-tetrahydropyrimidine-5-carbohydrazide(3). The parent compound 3 was used for synthesis of N’-substituted-4-(1H-indol-3-yl)-6-methyl-2-oxo- 1, 2, 3, 4-tetrahydropyrimidine-5-carbohydrazide and substituted aldehydes U (1-10), and/or Schiff bases of N’-substituted-4-(1H-indol- 3- yl)- 6- methyl- 2- oxo- 1, 2, 3, 4-tetrahydropyrimidine-5-carbohydrazide. Elemental analysis, FT-IR, 1H-NMR, and Mass spectral data supports the structures of synthesized compounds. The selected synthesized compounds were screened for...
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