Posted by admin on Mar 31, 2021 in |
The current pandemic COVID 19 caused by SARS-CoV-2 is very contagious and causes respiratory disease. Genomic analysis of different strains in SARS-CoV-2 were found to infect different geographical locations of the world (India, USA, and Brazil) and also in different parts of India (Kerala, Surat, and Ahmedabad). These sequences were downloaded from the viral genome sequence database of the National Center for Biotechnology Information (NCBI), and comparative analysis is performed using various computational tools. Comparison of genome size, G+C content, and identified ORFs is performed for different sequences of SARS-CoV-2 isolated from different geographical locations of the world and also from different cities/states of India using various bioinformatics tools. This study concludes that all the sequences isolated from different geographical locations are highly similar to the reference strain of Wuhan, with an identity percentage ranging from 99.56-99.98% and also with the same GC content of 37%. The difference in the identified ORFs of length >150 and a small difference in the identity percentages shows the presence of mutation between...
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Posted by admin on Mar 31, 2021 in |
Diabetes mellitus (DM) is a metabolic disorder in the endocrine system resulting from a defect in insulin secretion, insulin action or both of them. Adverse side effects of chemical drugs for the treatment of diabetes persuaded the use of medical plants. Macrotyloma uniflorum is a traditionally used plant for the treatment of diabetes is packed with powerful plant constituents with polyphenols, flavonoids, and proteins. They are one of the richest antioxidant sources, which lower blood sugar and bear other beneficial health effects. The purpose of this study is to evaluate the effect of ethanolic extract of Macrotyloma uniflorum leaves and stem (EMULS) on Alloxan-induced diabetic rats. In this study 36 Male Sprague Dawley rats, the body weight of 150-200 g was divided into 5 groups. Diabetes was induced by intraperitoneal injection of 150 mg/kg Alloxan. The Macrotyloma uniflorum treatment duration was 30 days in which two doses of extract (200 mg/kg & 400 mg/kg) were orally administered to diabetic rats. Blood glucose levels were estimated with glucometer before treatment,...
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Posted by admin on Mar 31, 2021 in |
Mebendazole is a broad-spectrum anthelmintic drug that is widely used in the treatment of helminthic infections. A simple, accurate, and precise UV-visible spectrophotometric method has been developed and validated for the determination of Mebendazole in tablet formulation. The solvent used for the development of the proposed method was Phosphate buffer 6.8 at 278.6 nm. The Beers’ law was obeyed in the concentration range 5-30 μg/ml. The developed method was validated as per ICH guidelines such as linearity, accuracy, precision, the limit of detection, the limit of quantification, and robustness. The correlation coefficient was found to be 0.999. The accuracy was found between 99.22 to 99.54%. The % RSD of Mebendazole was found to 0.05 to 0.20 for intraday and 0.05 to 0.15 for inter-day precision. The method was found to be precise as % RSD was found to be less than 2%. The LOD and LOQ were found to be 0.986 µg/ml and 2.988 µg/ml, respectively. The results demonstrated that the excipients in the commercial tablets did not interfere...
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Posted by admin on Mar 31, 2021 in |
The purpose of the present work was to prepare and evaluate olmesartan-loaded non-ionic surfactant vesicles (niosomes) to get sustained release by increasing the solubility and bioavailability. Ether injection and thin-film hydration methods were used for the preparation of all formulations as per the factorial design to study the effect of the three independent variables X1 (amounts of span 60), X2 (amount of cholesterol) and X3 (amount of chitosan/ PEG-6000) on two dependent variable Y1 (% DEE), and Y2 (% CDR), respectively. Prepared niosomes were characterized by % DEE, % CDR, FTIR, SEM, and zeta potential, etc. Statistical analysis was performed using ANOVA, and optimization was done by fitting experimental data. After 8 hours dissolution, the minimum and maximum cumulative drug release were observed to be 75.90% and 85.91%, and 77.32% and 88.74% for SPEIM-2 and SPEIM-1 and CHEIM-2 and CHEIM-1; 80.24% and 86.11% and 80.11% and 90.72% for SPTFH-2 and SPTFH-1, and CHTFH-7 and CHTFH-1, respectively. Data obtained from in-vitro dissolution tests were fitted to different kinetic models. FTIR...
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Posted by admin on Mar 31, 2021 in |
The present study was to estimate the total phenolic content, flavonoids content and evaluate the in vitro antioxidant activity of alcoholic and aqueous extract of the leaves of plant Mimusops elengi Linn. Total phenolic content was determined calorimetrically using Folin ciocalteu reagent, and Total flavonoid content was determined by aluminum chloride method. The total phenolic content of methanol, ethyl acetate soluble fraction, and aqueous extract was found 23.22, 22.44, and 15.88%w/w respectively. In the case of total flavonoid content was found 33, 31, and 24%w/w respectively. Antioxidant activity was measured based on the DPPH radical scavenging assay, Nitric oxide scavenging assay, and reducing power assay. A methanol extract of the leaves of the plant showed potent free radical scavenging activity with an IC50 value of 65.00 μg/ml. However, standard ascorbic acid activity was significantly higher than that of all extracts. The IC50 value of the standard (Ascorbic acid) was 7.779μg/ml. In the case of nitric oxide scavenging assay, the methanolic & aqueous extract of the leaves of the plant...
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