Posted by admin on Dec 31, 2020 in |
Introduction: Nymphaea pubescens Willd. is one of the well-known medicinal plants, being used from ancient times in India and commonly called “Kumuda”. It is an aquatic perennial herb. It has leaves having a smooth upper side, a strong root, and a branched rhizome. According to Bentham and Hooker classification “Genera Plantarum”, this plant belongs to the division Polypetale of Dicotyledones, which has been divided into 15 order and 84 families. Nymphaea comes under the Araceae family, which itself contains 8 genera and 100 species. Method: Morphological characters have been examined through a transverse section cut by microtome. Physiochemical studies and other screenings were performed accordingly. In addition to this, UV-VIS, TLC, FTIR, and HPLC tests were also carried out for further validation. Result: The morphological and physiochemical examination revealed the presence of saponins, proteins, and various other tests in Nymphaea pubescens Willd. Conclusion: The microscopic, UV-VIS, TLC, FTIR, and HPLC validation can be helpful for using it for medicinal and commercial...
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Posted by admin on Dec 31, 2020 in |
Environmental management is becoming the need of the hour due to rising pollution and environmental hazards. Incorporating green thinking into an organization will not serve the purpose in entirety. Therefore, we need to move towards greening the entire supply chain, here termed as Green supply chain management (GSCM). Adopting green supply chain management practices has been argued to enhance the environmental performance of many organizations, but its impact on the performance of the entire supply chain has not been studied so far in the context of the Indian pharmaceutical industry. The current study aims to identify the impact of GSCM practices on supply chain performance (SCP) and its ability to gain sustainable competitive advantage (SCA) and the mediating role of SCA on GSCM and SCP. For this, Structural Equation Modelling is applied using AMOS version 24. The results demonstrate a positive relationship between GSCM and SCA and SCP. This can prove beneficial to the managers of the pharmaceutical industry to justify the investments made in environmental initiatives and motivate...
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Posted by admin on Dec 31, 2020 in |
The copper (II) complexes, [Cu(phen)(tryp)I] (1), [Cu(phen)(tryp)Cl], (2) [Cu(phen)(tryp)SCN] (where phen = 1,10-phenanthroline and tryp = tryptophan ) has been synthesized and characterized by Infra-red Spectra, UV–Visible Spectra, EPR Spectra and elemental analysis methods. Binding interactions mode of the complexes with calf thymus DNA (CT-DNA) were investigated by UV–Vis absorp-tion titration, fluorescence emission, cyclic voltammetry, viscometric technique. The complexes were subjected to in-vitro cytotoxicity studies against the human liver cancer cell, as revealed by the MTT assay method. The complex was shown to be a partial intercalation mode of binding into DNA. The results show that complex 1 exhibited potent cytotoxic effects against human cell line (HepG2) when compared to complex 2 and complex 3. The complex was screened for its in-vitro antibacterial activity against one Gram-positive (staphylococcus aureus) and two Gram-negative (klebsiella pneumonia, Escherichia coli) bacterial strains and for in-vitro antifungal activity against (Aspergillus niger, Rhizopus species, Penicillium species). The results suggest that all the complexes exhibit good antibacterial activity. The antifungal activity data showed that complex 1...
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Posted by admin on Dec 31, 2020 in |
The aim of this study is to develop a sensitive, specific, rapid, and precise reverse-phase high-performance liquid chromatography (RP-HPLC) method and validate it for the quantification of process-related and degradation impurities of apixaban; an anticoagulant drug. The chromatographic separation was achieved on a Sigma-Aldrich’s Ascentis Express® C18 (4.6 mm × 100 mm, 2.7 µ) HPLC column with a runtime of 40 min. Mobile phase-A and mobile phase-B were phosphate buffer and acetonitrile, respectively. The column oven temperature was set at 35 °C, and the photodiode array detector was set at 225 nm. The newly developed method was utilized to detect nine process-related impurities (Imp-1 to Imp-9) in a test sample of Apixaban. Forced degradation study was carried out under acidic, alkaline, oxidative, photolytic, and thermal conditions to demonstrate the stability-indicating nature of the developed RP-HPLC method. The developed method was validated as per ICH guidelines and found to be specific, precise, sensitive, and robust. In conclusion, the RP-HPLC method was successfully developed and validated then effectively applied to analyze...
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Posted by admin on Dec 31, 2020 in |
Thrombocytopenia, a condition in which platelet count decreases (<150-450 × 109/L), occurs as a result of clinical conditions such as viral infections or cancer or as a side effect of chemotherapy or it can be inherited, but if not treated on time it can be life threaten-ing. So, here we have evaluated the anti-thrombocytopenic potential of three plant extracts (aqueous extracts of Carica papaya and Azadirachta indica, methanolic extract of Andrographis paniculata) individually and their equal, unequal, half, and double proportionate combinations against carboplatin induced thrombocytopenia in mice. Thrombocytopenia has been induced successfully in all the treatment groups as well as in the disease control group, followed by a single intraperitoneal injection of carboplatin at the dose of 125 mg/kg of body weight. The extent of thrombocytopenia was the highest on day 7. Platelet count was significantly low in the disease control group (p<0.0001) compared to all other groups starting from day 7 to the end. All groups have responded to their respective treatment and have shown an increase...
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