Posted by admin on Nov 30, 2020 in |
Penetrating biomolecules by ultrasonic techniques is an innovative and influential tool for characterizing their physicochemical properties. Peptides have been used as extensive applications in drug construction and as an ingredient in nutritional complements. An investigation based on the behavior of promulgation of ultrasonic waves in solids and the liquid system is now rather well recognized as an effective means for scrutinizing certain physical properties of the materials. The data attained from ultrasonic proliferation parameters such as ultrasonic velocity, density, viscosity, etc., and additional variation with concentration of one of the medium modules are useful in consideration of the nature of molecular interaction in terms of physical parameters. Owing to the sensitivity to very low populace densities at high energy circumstances, ultrasonic methods have been preferred and are testified to be corresponding to other techniques. An exhaustive study of literature exposes that the ternary solutions and liquid mixtures were premeditated and reported by different inventors. They testified on the nature of forces elaborate in the formation of the hydrogen bonds....
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Posted by admin on Nov 30, 2020 in |
The aim of this study was to screen the presence of bioactive compounds through GC-MS analysis and carry out the ant-inflammatory test of the methanolic tissue extract of Murex tribulus, a spiny predatory gastropod that is widespread from the Central Indian Ocean to Western Pacific Ocean. The GC-MS analysis of the methanolic flesh extract revealed the presence of four bioactive compounds, 1, 2 dimethyl hydrazine, urea, cis-9 hexadecenoic acid, and 1, 1, 1, 3, 5, 5, 5 hepta-methyltrisiloxane. Among the compounds identified, 1, 1, 1, 3, 5, 5, 5-Heptamethyltrisiloxane was the most abundant antimicrobial compound (92.87%) present in the methanolic tissue extract of M. tribulus. The results of the GC-MS analysis showed that the bioactive compounds present in the tissue extract showed anti-inflammatory responses. Hence in-vitro anti-inflammatory activity in the tissue extract of M. tribulus was determined by proteinase inhibitory activity and 5-lipooxygenase inhibitory assay. The anti-inflammatory test showed promising results with high percentage of inhibition such as 53.50% at a concentration of 1000 µg/ml in proteinase inhibitory activity...
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Posted by admin on Nov 30, 2020 in |
Urinary tract infection (UTI) is one of the common clinical conditions in patients presenting to the tertiary care hospital. In general, most of the case of UTI was being treated by empirical antimicrobial therapy before the laboratory results of urine culture; since the antimicrobial susceptibility test of uropathogenic bacteria and antibiotic resistance for enhanced management of UTI with a commonly used antibiotic. A prospective observational study was conducted on significant bacteria isolated from the urine samples submitted in microbiology unit from April 2017 to September 2017 at tertiary care hospital in Kathmandu, Nepal. E. coli (52.5%); the predominant etiological organism of UTI in our study showed high sensitivity to nitrofurantoin (93.3%), meropenem (92.9%), and colistin (82.3%). The most effective antimicrobial agent against Gram-positive Enterococcus species were linezolid (100%), vancomycin (100%) and nitrofurantoin (100%). A very high rate of resistance was seen against amikacin (75%), nalidixic acid (68.7%), and cotrimoxazole (63.9%) in Gram-negative bacilli isolates of E. coli. Escherichia coli, the most common uropathogens isolated more commonly from the female...
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Posted by admin on Nov 30, 2020 in |
A simple, accurate, and precise stability-indicating reverse phase high pressure liquid chromatographic method was developed and validated for the estimation of Perindopril arginine, Indapamide, and Amlodipine in the bulk and pharmaceutical dosage form. Chromatographic separation was carried out on ZORBAX RX C8 (250 mm × 4.6 mm, 5 µm) as a stationary phase with a mobile phase of gradient system of Phosphate Buffer with pH 2 containing Decane Sulphonate as ion-pairing agent (A) and Acetonitrile (B) at detection wavelength 215 nm with flow rate 1.0 mL/min at column temperature 40 ºC. The tR of Perindopril, Indapamide, and Amlodipine was 31.39 ± 0.21 min, 25.73 ± 0.41 min, and 36.95 ± 0.47 min, respectively. The method was linear over the concen-tration ranges 50-150 µg/ mL for Perindopril, 12.5-37.5 µg/ mL for Indapamide and 50-150 µg/ mL for Amlodipine. The LOD was 0.99 µg/ mL for Perindopril, 0.87 µg/ mL for Indapamide and 3.99 µg/ mL for Amlodipine. The LOQ was 3.02 µg/ mL for Perindopril, 2.6 µg/ mL for Indapamide...
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Posted by admin on Nov 30, 2020 in |
Permethrin, a member of one of the newest class of insecticides, the synthetic type-I pyrethroids, is very much in use nowadays. Due to its relatively low mammalian toxicity and high photostability, it has earned great popularity. Several reports suggest its endocrine disrupting activity, damaging effect on nervous system, its receptors, and other adverse effects. However, very limited information is available regarding its effect on reproductive organs. It is, therefore, imperative to bridge these lacunae. Hence, the present study was aimed to evaluate the effects of Permethrin on the reproductive tissues of male mice. Permethrin (130 mg/kg body weight) was administered orally for a period of 21 days. Biochemical parameters were assayed in testis, caput epididymis, cauda epididymis, seminal vesicle, and vas deferens of Swiss albino mice. The results showed that Permethrin administration lowered the gravimetric indices and decreased the levels of enzymes involved in the energy metabolism like ATPase, ALKPase, ACPase, and SDH, which may lead to oxidative stress and hamper the overall physiological...
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