Posted by admin on Dec 31, 2020 in |
The dried leaves of Merremia emarginata Burm. F were screened for antitubercular, cytotoxic, and anti-inflammatory activities. The antimycobacterial activity of compounds was assessed against Myco-bacterium tuberculosis using microplate Alamar Blue assay (MABA), and the cytotoxic activity was studied on Hela (Human cervical carcinoma), MCF-7 (Human breast carcinoma) cell lines. A prenylflavonoid, 8-prenylnaringenin was isolated on silica thin layer chromatography, and the radical scavenging efficiency of the isolated compound was evaluated by DPPH radical assay. The results demonstrated that the isolated compound was found to be greater than that of BHT from lower (0.25 mM) to moderate (2 mM) concentrations and comparable to BHT at higher concentrations, up to 4 mM. The flavonoid was also found to exhibit good antifungal activity when tested against Candida albicans (ATCC 2091) and Aspergillus niger (ATCC...
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Posted by admin on Dec 31, 2020 in |
We have explored the nanoneedles from nanofabrication techniques, which could penetrate both nuclear and cellular membranes of cells. We received the individual nanoneedles with a tip diameter of 220-250 nm and the desired microscale length ~1 to 100 μm, which enable them for transdermal delivery of selective drug molecule by piercing stratum corneum, the outermost layer of skin. These nanoneedles possess Young’s modulus ~100 GPa, which is necessary for mechanical stability of nanoneedles during its penetration across stratum corneum for transdermal drug delivery. On the prefabricated structure of silicon, the arrays of nanoneedles were grown. The nanoneedles were further bio-functionalised using conjugation of drug molecule like Insulin with PEG and successfully delivered into the rat’s skin. The nanoneedles could be act as a vehicle for transport of Insulin drug molecules. The surface morphologies, basic structure and composition of the nanoneedles were confirmed using SEM, TEM, FTIR, ICPAES, XRD and TGA. Super-conducting quantum interference device (SQUID) magnetometer was used to ascertain the magnetic properties of nanoneedles. In-vivo studies after administration...
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Posted by admin on Dec 31, 2020 in |
The main aim of the present research work is to develop a sensitive, precise and accurate HPLC (High-Performance Liquid Chromatography) procedure for the selective estimation of sonidegib in both human plasma and mobile phase. An isocratic separation of sonidegib through a 5µ Zorbax C-18 analytical column with the dimensions of 25 cm × 4.6 mm utilizing mobile phase composition of methanol, water and acetonitrile at a ratio of 10:10:80 v/v. The detection of the analyte was processed at the maximum wavelength of 254 nm and with 1 ml/min flow of the mobile phase. The drug was eluted from the column at the retention time of 3.6 min in plasma samples and 4.81 min in movable phase. Five variable concentration levels of 5, 10, 15, 20, and 25 μg/ml were used for the estimation of recovery and linearity. The recovery findings were 5.03, 9.93, 14.96, 20.14, and 24.91 μg/ml, respectively, for Sonidegib in the mobile phase. Similarly, 6 concentration levels of 20, 40, 200, 400, 800, and 1200 μg/ml were...
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Posted by admin on Dec 31, 2020 in |
Plant diseases caused by microbial pathogen leads to severe economic loss. Attempt to synthesize nanoparticles for this purpose is an economical and effective means to control these pathogens. For this purpose, nanoparticles synthesized via biological means are not only a safer choice but also a big step towards conservation of the integrity of environmental elements. In the present investigation, a successful attempt has been made to synthesize and characterize copper nanoparticles biologically synthesized from Lawsonia inermis leaf extract by an eco-friendly method. Formation of copper nanoparticles is confirmed by UV–Vis absorption spectroscopy, shape, and size studies were carried out Scanning electron microscopy (SEM), Transmission electron microscopy (TEM) analysis. TEM analysis revealed that copper nanoparticles were 2-5 nm in size and X-ray Diffraction studies confirmed the crystal structure of the nanoparticles. Functional groups and the chemical composition of associated molecules were studied by FTIR analysis. Further research involves the implementation of this research to control some plant diseases caused by pathogens that have developed resistance against present available bio-control...
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Posted by admin on Nov 30, 2020 in |
Sickle cell anemia is a major life-threatening hemoglobin disorder in the world. This hemoglobin disorder is an autosomal recessive type, therefore considered as a major health problem in the world. The pain symptoms and complications of sickle cell anemia can be life-threatening many times. Management of sickle cell anemia demands drugs to be safe, well-tolerated, lesser side effects, and effective in improving the quality of life in patients. The alternative system of medicine can be the fruitful therapeutic option in conditions where the drug has to be consumed for life long. The T-AYU-HM Premium is a novel traditional Ayurvedic medicine designed on such a purpose to reduce clinical complications and enhance the quality of life in sickle cell anemia patients. The study conducted was a single dose oral toxicity study of T-AYU-HM Premium to assess its safety profile at a high dose. The study was conducted in compliance with the OECD principles of GLP (1998). The study was conducted according to OECD for testing of chemicals, N-425 acute oral...
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