Posted by admin on Nov 30, 2020 in |
The objective of the present investigation was to formulate the thermosensitive ophthalmic in-situ gels of Dorzolamide HCl for the treatment of glaucoma. Dorzolamide HCl is presently available as conventional eye drops, which has demerits like pre-corneal elimination, lachrymal drainage and no sustained effect leading to poor bioavailability and hence there was a need to develop a better, stable and sustained drug release dosage form. Dorzolamide HCl loaded thermosensitive ophthalmic in-situ gels were prepared by cold method using poloxamers as thermo-sensitive polymers in different concentrations with HPMC K4M as viscosifier which was used in three different concentrations. A total of six formulations were made and evaluated for para-meters, namely appearance, clarity, pH, gelling capacity, gelation temperature, rheological study, drug content, and in-vitro drug release studies. All the evaluation parameters were within limits. Among all six formulations, one (DF-5) showed the highest drug release, and this formulation was evaluated for sterility, isotonicity, and in-vitro ocular irritation study, where all these parameters were found to be satisfactory. The results of the current...
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Posted by admin on Nov 30, 2020 in |
The medicinal plants, Adhatoda vasica, and Adhatoda beddomei are commonly used in the treatment of several illnesses and respiratory disorders viz., cold, severe cough, chronic bronchitis, asthma and tuberculosis. The present study aimed to identify the phytochemical constituents and quantification of bioactive contents of hexane extracts of these plants, followed by metabolic fingerprinting, which was carried out by GC-MS analysis. Assessment of phytochemical screening showed the presence of various phytochemicals like glycosides, phytosterols, flavonoids, terpenoids, and phenols in hexane extract of A. vasica and A. beddomei. A rapid method has been used for the comparative study of A. vasica and its related plant species A. beddomei using gas chromato-graphy-mass spectrometry (GC-MS). The phytocompounds comparison in two plants by GC-MS was similar in 12 compositions. Squalene, β-sitosterol, tetratetracontane, and lupeol were the major constituents identified in the hexane extract of A. vasica and A. beddomei. The extracts showed the presence of several phytoconstituents, including bioactive components, and provide reference data for future research of its active...
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Posted by admin on Nov 30, 2020 in |
The objective of the present study is to evaluate the phytochemical constituents, antioxidant and antibacterial activities of methanolic leaf extract of Clinopodium nepeta (L.) Kuntze. Total phenolic content of leaf extracts was assessed using Folin-Ciocalteu and AlCl3 reagent. Antioxidant assays were evaluated using DPPH, ABTS radical scavenging, and ferric, reducing antioxidant power (FRAP) methods. The leaf extract was screened against four pathogenic bacteria, two gram-positive (Escherichia coli, Bacillus cereus) and two-gram negative (Pseudomonas aeruginosa, Klebsiellea pneumonia) using disc diffusion method. The results showed the total phenolic content (378.52mg gallic acid equivalent (GAE)/g) and flavonoid content (189.4 mg quercetin equivalents (QE)/g) of methanolic leaf extracts. DPPH and ABTS radical scavenging activity with an IC50 value of 0.49mg/ml and 1.68mg/ml, respectively. It also exhibited good absorbance in the FRAP assay. The in-vitro antibacterial assay showed an excellent zone of inhibition against Pseudomonas aeruginosa (19.01±0.3mm), Bacillus cereus (24.66±0.3mm), Klebsiellea pneumoniae (16.74±0.3mm), and Escherichia coli (17.56±0.31). Clinopodium nepeta (L.) Kuntze showed maximum antioxidant property and maximum inhibition against the test organisms. The high...
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Posted by admin on Nov 30, 2020 in |
Nigella sativa (N. sativa) (Family Ranunculaceae) is a widely used medicinal plant throughout the world. Seeds and oil have a long history of folklore usage in various systems of medicines and food. Extensive studies of literature has been revealed that N. sativa has to carry antidiabetic, anticancer, immunomodulator, analgesic, antimicrobial, anti-inflammatory, spasmolytic, bronchodilator, hepatoprotective, renal protective, gastro-protective, antioxidant antihypertensive, liver tonics, diuretics, digestive, anti-diarrheal, appetite stimulant, analgesics, anti-bacterial properties, etc. Due to its miraculous power of healing, N. sativa has got a place among the top-ranked evidence-based herbal medicines. In the present study, we are reporting the anti-obesity activity of successive solvent extract by ethanol, Nigella sativa. The ethanolic extract of Nigella sativa was orally administered in high fat, high cholesterol diet (HFHCD) obese rats for about 12-14 weeks. After four weeks of treatment, blood samples were collected for the estimation of serum lipids, glucose, Total Cholesterol, HDL, LDL, and Triglycerides. The treatment with Nigella sativa extracts markedly lowered total cholesterol, triglycerides in blood serum. It also showed positive...
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Posted by admin on Nov 30, 2020 in |
The aim of this study was to prepare a suitable mucoadhesive in-situ gel formulation of clotrimazole for the treatment of vaginal candidiasis. The in-situ gel-forming systems are viscous liquids that turn to a gel phase upon exposure to physiological conditions. The lower retention of conventional formulations in the vagina, mucoadhesive formulations would prolong the retention time. For this study mixture of poloxamer (Plx) 407 and 188 were used. 32 full factorial design was applied for the optimization. Selected independent variables were concentration of carbopol 974 (X1), and Hydroxypropyl methylcellulose (HPMC) E50 (X2) was added to in situ gel for improve the mucoadhesive properties of the formulation, and prolong the residence time in vaginal cavity. Selected dependent variables were viscosity (Y1) release at 1 h (Y2), release at 12 h (Y3). After the preparation of mucoadhesive in-situ gels, gelation temperature/time, viscosity, mucoadhesive strength, spreadability, drug content, in-vitro drug release, and in-vitro antimicrobial efficacy were determined. Based on the obtained results and according to desirability, it was found that gel prepared...
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