Posted by admin on Oct 31, 2020 in |
A new stability-indicating high-performance liquid chromatographic method of analysis of capecitabine in pharmaceutical dosage form was developed and validated. The solvent system consisted of methanol: water (60:40% v/v). The retention time of capecitabine was 7.927 min at a flow rate of 1 ml/min on C-18 (Quails BDS, 250 × 4.6 mm, 5µ). Capecitabine was detected at 240 nm. The linear regression analysis data for the linearity plot showed good linear relationship with the correlation coefficient value, R2 = 0.9991 in the concentration range 4-50 µg/ml. The method was validated according to the ICH guidelines. The accuracy of the method was validated by recovery studies and was found to be significant and under specification limits, with Recovery 98.12-101.61%. The assay of capecitabine was determined in tablet dosage form. The method was also found to be robust. The drug was subjected to stress conditions of acidic, basic, oxidation, photolytic and thermal degradation, and considerable degradation was found in all stress conditions. The method has proven specificity for the stability-indicating method....
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Posted by admin on Oct 31, 2020 in |
Herbs have been used since antiquity for treatment and management of various disease conditions. Their ability to cure or prevent diseases is due to storehouses of various phytoconstituents like alkaloids, flavonoids, glycosides, terpenoids, steroids, tannins, phenols, etc. present in them. Pentacyclic triterpenoids have gained greater attention of researchers due to its wide array of pharmacological actions. Thus, a simple and precise HPTLC method has been developed for identification and quantification of one such ubiquitous pentacyclic triterpenoid- ursolic acid in the leaves of Alstonia scholaris. The optimized mobile phase Toluene: Ethylacetate (7:3v/v) gave a better resolution of bands of ursolic acid at 540 nm after derivatizing plate with ASR. The Rf value for ursolic acid was found to be 0.46. The amount of ursolic acid quantified in ethanol, and chloroform extracts of leaves of Alstonia scholaris were 2.34 and 2.38 %w/w, respectively. The developed method was validated in terms of linearity, range, specificity, precision, accuracy, LOD, and LOQ following ICH guidelines. The calibration curve of ursolic acid was linear between...
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Posted by admin on Oct 31, 2020 in |
1-{3-[2, 3dihydro-1H-imidazol-1-yl (8-hydroxyquinolin-7-yl) methyl] phenyl-l} ethanon, nitrogen and oxygen-based bi-dentate mannich base heterocyclic ligand synthesized and complexed with biologically significant transition metals like copper, cobalt, nickel, and zinc by the melt condensation three-component one-pot synthesis. All the synthesized potential compounds characterized by spectral methods by using NMR, IR, UV-visible mass, and elemental analysis. The antimicrobial analysis investigated for all the synthesized compounds using some bacterial and fungal cultures used by the disc diffusion method, and the activity proved in proportion with the standard drugs used as a reference. The main significant part of the work involved in mannich base reaction of one-pot synthesis three-component reactions prove better synthetic procedure and improved product yield. The ligand and the complexes of transition metals proved better microbial activities of biologically important and to prove the efficiency of the present work. Continuation of research work is to investigate significant heterocyclic molecules and transition metal complexes to prove potential microbial activities play an important role in pharmaceutical and biological...
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Posted by admin on Oct 31, 2020 in |
In the modern lifestyle, people are prone to disease. The epidemiology study shows that the lung and colon cancer leading position in both the gender around the world at any group. As based on the traditional systems of medicine, the plants can be used for the discovery of new medicine. The aqueous and ethanol extract of Nigella sativa (N. sativa) seeds were evaluated for their genotoxicity using Ames test and cytotoxicity by MTT assay. Ames assay was conducted using strains of Salmonella typhimurium TA-100, whereas MTT assay was performed on human alveolar basal epithelial cells (A549) and human colon cancer cell line (HCT15). There is no mutagenic activity was obtained when treated with aqueous and ethanol extract of Nigella sativa seeds. But both the extracts were found to be cytotoxic against the tested cancer cell lines. On the basis of the results, it was concluded that aqueous and ethanol extracts of Nigella sativa seeds possess cytotoxic potential but not mutagenic. It is suggested to explore the seeds further to...
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Posted by admin on Oct 31, 2020 in |
Zinc oxide-magnesium has been used in dentistry with limitations especially related to its high acidity and low biocompatibility. The objective of this study was to develop cement based on nano-hydroxyapatite (nHA), chitosan as more biocompatibility agents to increase zinc oxide-magnesium cito-compatibility. The formulations prepared were: 1. Complete cement (HCP) with nHA, zinc oxide-magnesium, and chitosan; 2. cement without nHA (CP); 3. Without chitosan (HP); 4. Without zinc oxide-magnesium (HC), and CPZn as control. All cement were compared for setting times, solubility, contact angle, pH, calcium ion release, shear strength, the thermogravimetry analysis, X-ray diffraction, Raman spectroscopy, 3T3-L1 fibroblasts cell compatibility, and antimicrobial activity. The results showed that HCP cement had setting time (mean ± S.D) of 5.5 ± 0.18 min, (p≥0.05) and neutral pH values on 14 days, calcium release of 10 mgL-1, and solubility with a value of 2.15%. The physicochemical characterization showed an expected pattern from the raw materials and composition. The values of the contact angle measurements indicated increased wettability for the HCP than the control...
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