Posted by admin on Oct 31, 2020 in |
Objective: The study was aimed to isolate the components from the methanolic extract of the root bark of Aphanamixis polystachya and to confirm their potential cytotoxicity of the methanolic extract, silver nanoparticles and silver nanosuspension of the root bark of A. polystachya. Method: Isolation from the methanolic extract of root barks of A. polystachya were performed using column chromatography technique. Cytotoxic activity on MCF-7 and MDA-MB cell lines by SRB Assay was conducted on silver nanosuspension of A. polystachya. Results: The components isolated from methanolic extract of root bark of A. Polystachya by column chromatography were confirmed as Rohituka 7, Rohituka 3, Amoorinin-3-o-α-l-Rhamnopyranosyl-(1à6)–β-D-Glucopyra-noside and 8- Methyl-7, 2׳,4׳-tri-O-Methylflavonone-5-O-α-L-Rhamnopyranosyl (1 4)-β-D- glucopyranosyl-(1 6)-β-D-glucopyranoside on the basis of IR, 1HNMR, 13C NMR and MS. The prepared silver nanosuspension, tested in concentrations ranging between 500-7.81 µM showed IC50 value at 0.58 µM in MCF-7 cell line, and IC50 in MDA-MB cell line being greater than 1000µM. Conclusion: Column chromatography of the methanolic extract of root barks of A. polystachya led to the isolation...
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Posted by admin on Oct 31, 2020 in |
Retinal ganglion cell (RGC) death is distinctive of many ophthalmic disorders, such as glaucoma, optic neuropathies, and various retinovascular diseases. N-methyl-D-aspartate (NMDA)-type glutamate receptor (NMDAR)-mediated excitotoxicity is thought to be an important contributor to RGC death. Uninterrupted activation of a large number of NMDARs leads to increases in intracellular calcium loads, initiating a cascade of events that eventually result in apoptosis or necrosis. We aim to prevent or delay RGC death by an intervention involving lithium to afford neuroprotection of RGCs or neutralization of the deleterious effects of toxic factors. Existing reports highlight a range of lithium dosage applied from 7 to 21 days to promote the survival of RGCs damaged by optic nerve injury. Lithium was injected continuously for 7 days at a dose of 30, 50, and 70 mg/kg body weight (b.w.) to Wistar rats after injecting 20 mM NMDA intravitreally. Morphological changes observed by hematoxylin and eosin (H & E) staining along with measurement of the thickness of the inner retina (IR) , made up of...
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Posted by admin on Oct 31, 2020 in |
Here, we report the in-vitro and in-vivo effects of Valspodar (PSC833), a second-generation mammalian efflux pump inhibitor on the susceptibility of methicillin-resistant and methicillin-sensitive Staphylo-coccus aureus (MRSA and MSSA) towards two antibiotics, Oxacillin (OX) and Cefoxitin (FOX). Checkerboard microdilution assays revealed various degrees of strain-dependent synergy demonstrated in the form of major reductions in antibiotic Minimum Inhibitory Concentrations (MIC) and Valspodar Minimum Effective Concentration (MEC). Fractional Inhibitory Concentration (FIC) for OX-PSC833 tested against MRSA and MSSA were 0.125 and 0.048, respectively, and for FOX-PSC833 was 0.5 for the MSSA. In-vivo studies showed that using a combination of an antibiotic and Valspodar to treat bacteremia induced by the different strains of S. aureus in a mouse model did not achieve complete killing of the MRSA strains, but resulted in a significant reduction in bacterial counts. MEC of PSC833 achieving synergy with antibiotics was as low as 5.2 µg/ml. Valspodar is a good candidate for antibiotic combination therapy at concentrations that are considered safe for human...
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Posted by admin on Oct 31, 2020 in |
Tecomella undulata (Sm.) seem is a monotypic genus belonging to family Bignoniaceae. The plant holds the tremendous potential of medicinal value and has been traditionally used in various ailments like syphilis, leukoderma, blood disorders, to name a few. The plant has gained prominence due to the presence of some prominent secondary metabolites. The present study focuses on the GC-MS analysis of extracts of all the plant parts of T. undulata, which revealed the presence of certain bioactive compounds like Quericitin, Kaempferol, stigmasterol, sitosterol, thiazoline, phytol, pthalic acid, methyl alpha ketopalmitate and so forth. A total of about 20 bioactive compounds were identified. The antimicrobial activity of the extracts was assayed against pathogenic bacteria and fungi. The flavonoids from leaf extracts showed highest antimicrobial activity against C. albicans, S. aureus, E. coli, and B. subtilis. This also correlates with the highest amount of Flavonoids present in leaves followed by the amount present in roots. The study thus infers that the presence of bioactive components may be the principle behind the...
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Posted by admin on Oct 31, 2020 in |
A Simple, sensitive, specific and stability indicating RP-HPLC method was developed for the simultaneous estimation of velpatasvir and sofosbuvir in bulk and tablet dosage form. Effective separation attained by injecting 10 μL of the standard solution containing velpatasvir and sofosbuvir into Xbrdige Phenyl (250 × 4.6 mm, 5 µ, 100 A0) column, with a mobile phase containing methanol, buffer (0.1% formic acid in water) and acetonitrile in 40:30:30 v/v ratio, at a flow rate of 1ml/min. The eluted analytes were effectively responded qualitatively and quantitatively at 273 nm wavelength. The drugs were experienced in stress conditions such as oxidative, acid, base, photolytic, and thermal degradation. The retention times of sofosbuvir and velpatasvir were observed at 3.4 and 5.1 min, respectively. The method has a good linear response in the concentration range of 10-30 µg/ml and 40-120 µg/ml for velpatasvir and sofosbuvir respectively. LOD and LOQ were calculated as 1 µg/ml and 4 µg/ml for velpatasvir, 4 µg/ml, and 13 µg/ml for sofosbuvir. All the obtained validation parameters have been...
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