Posted by admin on Sep 30, 2020 in |
Herbal medicines play an important role in cancer prevention. Mangroves have been used worldwide due to the presence of various bioactive metabolites in it. The specific medicinal properties of Rhizophora mucronata have been an interest in preventing breast cancer. Mangroves Rhizophora mucronata belongs to family Rhizophoraceae. The leaf extract reveals various roles in folk remedies to treat various diseases. This analytical study aims to find out various phytochemical and components present in the leaf extract by the GC-MS technique. The methanol leaf extract revealed the presence of phytochemicals like saponins, flavonoids, alkaloids, quinines, glycosides, cardiac glycosides, terpenoids, phenols, coumarins, and steroids. The GC-MS analysis provided nine different compounds like Phenol, 2, 4-bis (1,1-dimethyl ethyl)- (28.2%), Flavone- (66.9%), 4H-1-benz0pyran-4-one,7-hydroxy-2phenyl (39.8%), Chrysin (100%), 4H-1 Benzopyram-4-one, 7-hydroxy-2-phenyl-(100%), 5,8 Octadecadienoicacid, methyl ester-(55.8%), Phytol- (31.8%), Coumarine, 3-(2,4-dinitrophenyl)-(31.3%), Elaidic acid, isopropyl ester-(9.2%). Further purified compounds are used in the pharmacological industry against breast cancer and reduce the side effects and pains during the treatment of...
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Posted by admin on Sep 30, 2020 in |
Adaptation of marine actinobacteria to extreme climatic conditions such as high salinity, high pressure and high temperature has modified their physiological conditions to survive and elaborate novel bioactive metabolites. Among the different actinobacterial strains isolated from coastal regions of Guntur District, Andhra Pradesh, India, one potent strain with broad-spectrum antagonistic activity was found and identified as Streptomyces rectiverticillatus VJMS-8. Attempts were made to optimize the cultural parameters for an enhanced yield of antimicrobial metabolite production. Production of bioactive metabolites by the strain was high in tryptone yeast extract dextrose broth as compared to the other media tested. The strain utilized galactose and tryptone as good carbon and nitrogen sources for the elaboration of bioactive metabolites. The optimum temperature and pH for bioactive metabolite production by the strain were recorded as 35 °C and 7.0, respectively. NaCl @ 5% supported high pro-duction of bioactive metabolites. The secondary metabolites produced by the strain grown under optimal conditions exhibited high antagonistic activity against a variety of gram-positive and gram-negative bacteria as well...
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Posted by admin on Sep 30, 2020 in |
The objective of present research work is design and in-vivo evaluation of ibrutinib self-nanoemulsifying drug delivery system (SNEDDS) for the enhancement of oral bioavailability. Box-Behnken design was used for the study and the results analysed using response surface methodology. Ibrutinib SNEDDS were prepared with various oils, surfactants and co-surfactants and tested particle size, PDI, zeta potential, refractive index, drug release and TEM studies. The optimized ibrutinib SNEDDS has the composition of capryol 90, cremophore EL and transcutol HP. Based on the particle size and entrapment efficiency and in-vitro dissolution studies; F2 is identified as optimized formulation with 85% drug released, whereas less than 1% was released from pure drug at end time of 30 min. In-vivo bioavailability data reported in male wistar rats indicate higher concentration of drug in plasma implying enhanced systemic absorption of ibrutinib from SNEDDS formulation. The Cmax and AUC0-inf values of optimized SNEDDS was considerably higher (p<0.05) than pure drug formulation. The oral bioavailability of optimized SNEDDS increased around 12 folds when compared with pure...
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Posted by admin on Sep 30, 2020 in |
The work was initiated to induce a toxic model of a neurodegenerative disorder with Alzheimer’s disease symptoms in rats and to study the effect of quercetin, an anti-oxidant, and nerve growth factor on some biochemical parameters and parameters of behavioral performance of the animals. It was established that upon induction of the neurodegenerative disorder by an intranasal administration of aluminum chloride and lypo-polysaccharide, there was a decline in motor-sensor, emotional/motivation and cognitive parameters of the animals’ behavioral performance. An increase in the neutral and some fractions of glycolipids, as well as changes in phospholipid profile, were found; the activity of the antioxidant system enzymes with the MDA increase in the hippocampus of the animals was found to decrease. The deviations above in the biochemical parameters of the animals’ hippocampus seem to be a cause for the changes in the animals’ behavioral performance. Intranasal administration of the anti-oxidant and NGF in the liposomal form partially restored the parameters of the animals’ behavioral performance and the biochemical parameters to the control...
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Posted by admin on Sep 30, 2020 in |
A simple, sensitive, and rapid reverse phase High-Pressure Liquid Chromatography (HPLC) method was developed and validated for the quantification of residual Dicyclohexylurea in Fosaprepitant dimeglumine drug substance at trace level. The method was optimized on Zorbax Eclipse XDB-C8, 250 mm × 4.6 mm, 5µm with column oven temperature maintaining at 35 °C with flow rate at 0.7 ml/min. Mobile Phase was prepared by a mixture of phosphate buffer pH 7.0 (0.68 g of Potassium dihydrogen orthophosphate in 1000 ml of water) and Acetonitrile in the ratio of 53:47 v/v), the injection volume is 20 µl, the detector wavelength is 205 nm. The developed, optimized method was further validated in accordance with ICH, and the method is found to be specific, sensitive, accurate, and precise. The Limit of detection (LOD) and Limit of quantification (LOQ) for dicyclohexylurea are 20 µg/g and 60 µg/g, respectively. The method validation experimental results are discussed in detail in this research...
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