Posted by admin on Aug 31, 2020 in |
A simple, sensitive, and rapid Liquid Chromatography-Mass Spectroscopy method was developed and validated for the quantification of Isoproterenol in human plasma using Dobutamine as an internal standard. The method utilized simple liquid-liquid extraction using a mixture of diethyl ether and dichloromethane for the sample preparation involved prior to LCMS analysis. The analytes were chromatographed on Prontosil ODS C18 Column with isocratic elution using methanol, Acetonitrile and Tri ethyl-amine in the ratio of 60:25:15 (v/v) at pH 6.3 as the mobile phase at a flow rate of 0.9 mL/min and the UV detector response of the column eluents were recorded at a wavelength of 248 nm. Quantification was performed in multiple-reaction-monitoring mode with the ion transitions m/z 212.19 → 135.21 for Isoproterenol, m/z 302.19 → 107.05 for Dobutamine. Good linearity was obtained in the range of 0.50–300 ng/mL (r2 = 0.999). The method was fully validated with accuracy, precision, matrix effects, recovery and stability. The results of the stability study confirm that the method was found to be stable. The...
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Posted by admin on Aug 31, 2020 in |
In this planed research work, aryl piperazine derivatives will be synthesized because aryl piperazine currently the most important constructive block in drug discovery with positive pharmacological evaluation. A series N- (4- (benzo[d]thiazol-2-yl) phenyl)-2-[4-(aryl-substituted) piperazines-1-yl]acetamide, N-(4-(benzo[d]oxazol-2-yl)phenyl)-2-[4-(arylsubstituted)piperazines-1-yl]acetamide and Synthesis of N-(4-(benzo[d]imidazol-2-yl) phenyl)- 2- (4- (arylsubstituted)piperazin-1-yl) acetamide will be synthesized with their characterization such as melting point determination, Thin-layer chromatography (TLC) and spectral analysis. After that, pharmacological evaluation, such as antibacterial activity, will be performed for synthesized compounds. All the synthesized compounds were reported for determination of zone of inhibition (mm), minimum inhibitory concentrations (MIC) were also calculated for effective derivatives, with an objective to offer some potent antimicrobial agents to human beings. Antimicrobial activity is determined based on their in-vitro activity in pure cultures. In-vitro susceptibility testing is done by two methods i.e., Turbidimetric/photometric/tube dilution method and agar diffusion/cup-plate/cylinder plate...
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Posted by admin on Aug 31, 2020 in |
SARS-CoV-2 or COVID-19 is one of the deadly pandemics faced by the world population, which has infected 7 million and claimed the lives of 0.4 million people. In spite of a few drugs available to control the pandemics, a formal vaccine is the least that the world expects under the current scenario. However, the release of a vaccine is expected to come at the cost of its own time. SARS-CoV-2 replicates in the host cells with the aids of the molecular machinery of a complex formed by three non-structural proteins (NSPs) viz., nsp12, nsp8, and nsp7. Recent studies reveal that among the three NSPs, nsp12 is vital for viral replication and is the target for drugs. Several studies have linked the viral infection to a weaker immune system, which is quite likely to be targeted by the virus. In search of such a natural compound that might increase the immunity and block the viral replication within the host, we selected C-Phycocyanin of Spirulina plantesis to study its anti-viral property...
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Posted by admin on Aug 31, 2020 in |
This experimental study had approved by animal ethics of Central Drug Research Institute, Lucknow, and had undertaken to evaluate the antidyslipidemic and anti-oxidant activities of Nigella Sativa (N. Sativa), Hindi name Kalonji seeds extract in two models of hyperlipidemia. 1- triton and 2- cholesterol-rich high-fat diet (HFD) induced hyperlipidemia. N. Sativa and Gemfibrogil were macerated with 2% aqueous gum acacia, and the suspension had fed orally to rats at a dose of 500 mg/Kg (b.w.p.o.) respectively. Serum lipids were found to be lowered by N. Sativa in triton induced hyperlipidemia. On the other hand chronic feeding of N. Sativa extracts to rats in cholesterol-rich high fat diet-induced hyperlipidemia for 30 days caused lowering in lipid and apoprotein levels of β-lipoproteins followed by an increase in lipid and apoprotein levels of α lipoproteins. The results of the present study also demonstrate that N. Sativa seeds extract repaired hepatic lipid synthesis, increased fecal bile acid excretion, and increased plasma LCAT activity in rats. Furthermore, N. Sativa seeds extract (100 to 400...
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Posted by admin on Aug 31, 2020 in |
In the present study, an eco-friendly, cost-efficient, rapid and simple approach was applied to the synthesis of silver nanoparticles by using a methanolic extract of bulb of Zephyranthes citrina. The metal salt was reduced to ions by metabolites present in bulb extracts. The plant extracts acts both as a reducing agent as well as a capping agent. The synthesized silver nanoparticles were characterized by various spectral techniques like UV-Visible, FT-IR, SEM, EDAX and XRD analysis. Biosynthesis of silver nanoparticles was confirmed by viewing the colour change of the extract from brown to blackish brown. The functional group which is responsible for the reduction of silver ions in the plant extract was characterized by FT-IR. UV-Visible spectrophotometer showed an absorbance peak at 277 nm for silver nanoparticles. The SEM image depicts the nanosized particles with morphological shape. The elemental composition was determined by EDAX analysis. The XRD results confirmed the crystal structure of the nanoparticles. The spectral data confirm the formation of nanoparticles with its size, morphological structure and chemical...
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