Posted by admin on Aug 31, 2020 in |
The L-Glutaminase enzyme is a therapeutic agent that can be employed for the treatment of human cancer, specifically lymphocytic leukemia. Microbes are exploited commercially to produce L-Glutaminase on a large scale. The L-Glutaminase is an economic anti-cancer drug and can be easily administered into patients. The L-Glutaminase is also employed in food industries in the processing of fermented foods. In the present paper, the L-Glutaminase enzyme structure was developed and validated using web-based computational tools. The sequence (FASTA format) of the L-Glutaminase enzyme was obtained from UniProt Knowledgebase. The FASTA format sequence of the L-Glutaminase enzyme was used as input data in the SWISS MODEL workspace to develop the structural model of L-Glutaminase by automated mode. The quality of the modeled structure of the L-Glutaminase enzyme was checked in PROCHECK server and SPDBV (Swiss PDB Viewer) and validated. Further, L-Glutaminase activity can be enhanced using advanced bioinformatics tools. Such L-Glutaminase with improved activity can be produced by...
Read More
Posted by admin on Aug 31, 2020 in |
Notable progress has been made in methods that encourage the use of PCR as a rapid and accurate tool in quality evaluation of pharmaceuticals. In this study, monoplex and multiplex PCR based assays were developed and compared with standard conventional methods for rapid detection of three specified topical indicator pathogens, Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans, in nonsterile pharmaceutical preparations. The detection limit of monoplex PCR assays for the microbial targets was achieved at 100 fg purified DNA and 10 CFU/ml for Pseudomonas aeruginosa, and 1fg purified DNA and 10 CFU/ml for each of Staphylococcus aureus and Candida albicans. No change in the detection limit for CFU/ml of the three tested indicator pathogens was obtained upon using mPCR assays. The results of applying both conventional and PCR detection methods for different cream and lotion preparations revealed a 100% correlation between both methods. The PCR based detection method can be completed in 8 h versus 5-6 days in case of conventional methods, but the former can’t differentiate between viable...
Read More
Posted by admin on Aug 31, 2020 in |
Background: Self-Nanoemulsifying Drug Delivery System (SNEDDS) has been employed extensively by the formulation of scientists to tackle the low solubility issues of various drugs and lift the bioavailability profile. But the potential of SNEDDS is not limited to augment the dissolution profile only. Objective: The objective of the study was to develop, optimization, and characterization of SNEDDS of anti-lipase inhibitors. Method: Orlistat was added in an accurately weighed amount of oil into a screw-capped glass vial and heated in a water bath at 40 ºC. The surfactant and co-surfactant were added to the oily mixture using positive displacement pipette and stirred with a magnetic bar. The formulation was further sonicated for 15 min and stored at room temperature until its use in subsequent studies. A 23 full factorial design was selected because an experiment may be designed to focus attention on a single independent variable or factor. Results: The drug loading efficiency for all Orlistat SNEDDS formulae was found in the range of 92.37% ± 0.75% (PF1) to 99.09%...
Read More
Posted by admin on Aug 31, 2020 in |
Anti-inflammatory activity and the toxicity of Bohadschia vitiensis water extract were investigated by in-vitro models to evaluate the consumption of the extract by the local community to reduce arthritic-related pain. Specimens of B. vitiensis were collected from Mannar, Sri Lanka, and the water extract (WE) was prepared by removing visceral organs and incubating diced samples in distilled water, followed by freeze-drying. The WE was used to test acute toxicity, based on Danio rerio embryotoxicity assay and to evaluate anti-inflammatory activity based on in-vitro models; egg albumin denaturation, erythrocyte membrane stability, Nitric Oxide (NO) scavenging and Hydrogen Peroxide (HP) scavenging activities. The IC50 was calculated for each assay and compared with a standard reference drug. The qualitative zoo-chemical analysis was carried out while the presence of major nutrients was evaluated. According to the results, WE had a LC50 of 151.59 µg/ml for Danio rerio embryotoxicity assay. The anti-inflammatory activity against egg albumin denaturation (IC50 of 277.51 µg/ml) was reported while a maximum percentage inhibition of 32.09% was reported at 250...
Read More
Posted by admin on Aug 31, 2020 in |
Catalase, guaiacol peroxidase and polyphenol oxidase are antioxidant enzymes that are important in the metabolism of reactive oxygen species and can be induced by environmental stresses including chromium (Cr), heavy metal toxic to living organisms. In-vitro regenerated shoots of carnation (Dianthus caryophyllus L.) were exposed to K2Cr2O7, and the activities of these antioxidant enzymes were analyzed. A significant reduction in the POD activity was observed at 2nd week of culture, followed by a slight increase in the activity in the 4th week of culture. Maximum PPO activity was detected during the 3rd week of culture, which was comparable to the control cultures. A significant reduction in CAT activity was found in the 4th week. The chlorophyll content did not change significantly in the shoot buds developed up to 4th week of...
Read More
