Posted by admin on Sep 30, 2020 in |
New simple, sensitive, and validated stability-indicating RP-HPLC method has been developed for the simultaneous estimation of Rilpivirine and Dolutegravir in its bulk form. Chromatographic separation was achieved on a Hypersil ODS (250mm × 4.6mm i.d., 5µm) maintained at ambient temperature by a mobile phase consisted of methanol and water (80:20v/v) and a flow rate of 1.0mL/min with a load of 20µL. The detection wavelength was set at 282nm. The retention time for the drugs was found to be Rilpivirine (5.14min), Dolutegravir (6.72min). The eluted compounds were detected using a UV detector. The drugs were subjected to stress degradation as per ICH Q1A. There was the interference of degradant at RT of Rilpivirine and Dolutegravir. The developed method was successfully validated according to ICH guidelines. The calibration curve was found to be linear over a range of 10-100µg/mL. The accuracy of the method is indicated by a good recovery in the range of Rilpivirine 99-102% and of Dolutegravir 99-102%. The limit of detection and limit of quantification of Rilpivirine was...
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Posted by admin on Sep 30, 2020 in |
The present study was undertaken to systematically analyze, document the traditional knowledge of Clerodendrum species use for the treatment of various human ailments from NER. The information was collected by literature survey as well as by consulting questionnaire with the villagers and local communities of NER. A total of twelve Clerodendrum species were collected, and among them, C. colebrookianum, C. indicum, C. viscosum were found to have multi-medicinal values that were widely used for the treatment of various diseases. Further antidiabetic properties of Clerodendrum species were evaluated by α-amylase and α-glucosidase assay. The result showed 4 species, C. indicum, C. japonicum, C. serratum, C. viscosum exhibited significant (>50%) α-amylase inhibition properties while 3 other species, C. colebrookianum, C. inerme, C. viscosum displayed significant α-glucosidase inhibition properties. Lowest IC50 values were observed in C. serratum of α-amylase (45µg/ml) and C. viscosum of α-glucosidase (47 µg/ml). A detailed scientific investigation of the biochemical compounds and metabolites with potential biological activities in Clerodendrum species may lead to the discovery of potential drug...
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Posted by admin on Sep 30, 2020 in |
Background: Prevention of recurrence is important for patients with ischemic stroke. The purpose of the study was to compare the effectiveness of two antiplatelet regimens-aspirin plus clopidogrel versus aspirin plus cilostazol in early secondary stroke prevention using National Institutes of Health Stroke Scale (NIHSS) Scores. Methods: In this study, we randomly assigned 30 patients to receive aspirin plus clopidogrel once daily or to receive aspirin once daily plus cilostazol twice daily. The patients were followed up for a period of three months, and the NIHSS scores were taken on admission, and every month thereafter. After completion of the study duration, the scores of both the groups were compared, and results were interpreted. Results and Discussion: More number of patients were found to be in the age group of 60- 70 years with a mean age of 63 years. In both the groups, the percentage of females was higher than that of males. Hypertension (80%) and diabetes mellitus (56.6%) appeared to be the major risk factors. The percentage of death...
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Posted by admin on Sep 30, 2020 in |
Michelia champaca known as champaca is belonging to family of Magnoliaceae. The present work consist of extraction and evaluation of anti-asthmatic activity of flowers of Michelia champaca Linn. Flowers was evaluated for anti-histaminic activity using isolated goat tracheal chain preparation and histamine induced Broncho-constriction in Albino mice. Michelia champaca Linn. expressively inhibited dose reliant contraction of goat tracheal chain produced by histamine and also exhibited significant protection by prolonging preconvulsion dyspnea time (PCD) in mice. Thus, Michelia champaca Linn. showed anti-allergic activity against histamine and hence possesses potential role in the treatment of...
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Posted by admin on Sep 30, 2020 in |
Penetrating biomolecules by ultrasonic techniques is an innovative and influential tool for characterizing their physicochemical properties. Peptides have been used as extensive applications in drug construction and as an ingredient in nutritional complements. An investigation based on the behavior of the promulgation of ultrasonic waves in solids and the liquid system is now rather well recognized as an effective means for scrutinizing certain physical properties of the materials. The data attained from ultrasonic proliferation parameters such as ultrasonic velocity, density, viscosity, etc. and additional variation with a concentration of one of the modules of a medium are useful in consideration of the nature of molecular interaction in terms of physical parameters. Owing to the sensitivity to very low populace densities at high energy circumstances, ultrasonic methods have been preferred and are testified to be corresponding to other techniques. An exhaustive study of literature exposes that the ternary solutions and liquid mixtures were premeditated and reported by different inventors. They testified on the nature of forces elaborate in the formation...
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