Posted by admin on Sep 30, 2020 in |
Objective: The aim of this study was to screen various solvent extracts of leaves of Rumex hastatus to display in-vitro antioxidant activity, total phenolic, and flavonoid contents in order to find possible sources for future novel antioxidants in food and pharmaceutical formulations. Materials and Methods: A detailed study was performed on antioxidant activity of various Rumex hastatus leaves extracts like petroleum extract (PERH), chloroform extract (CERH), ethyl acetate extract (EAERH), methanolic extract (MERH) and aqueous extract (AERH) by in-vitro chemical analysis like DPPH, Reducing power, metal chelating, hydrogen peroxide and nitric oxide free radical scavenging assay. All these extracts were also subjected to a preliminary phytochemical screening test for various constituents. The total yield, total phenolic, and total flavonoid contents of all extracts were also determined. Results: Phytochemical screening shows the presence of triterpene, flavonoids, polysaccharides, steroids, tannins, cardiac glycoside, reducing sugar, and saponins in all extracts. The MERH exhibited the highest total phenolic content (121.7 ± 5.95 mg of GAE/g extract) and total flavonoid content (165.9 ± 3.99...
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Posted by admin on Sep 30, 2020 in |
A Simple and trace level-sensitive Gas chromatography with mass spectrometer (GCMS) method was developed, optimized and validated for the determination of seven potentially genotoxic impurities which are having structural alerts i.e., 1-Bromo-5-chloropentane (PGI-1), Ethyl-6-chloro-2-oxo hexanoate (PGI-2), Methyl-7-chloro-2-oxo hepta-noate (PGI-3), Ethyl-7-chloro-2-oxo heptanoate (PGI-4), Ethyl-7-bromo-2-oxo heptanoate (PGI-5), Ethyl-8-chloro-2-oxo octanoate (PGI-6), Ethyl-2-acetyl-7-chloro heptanoate (PGI-7) contents in Cilastatin sodium drug substance. The chromatographic separations of seven genotoxic impurities were achieved on ZB-5 MS [5% Polysilarylene, 95% Poly-dimethylsiloxane copolymer capillary column of 30 m length, 0.32 mm internal diameter, film thickness 1.0 µm]. The analytic method has been demonstrated through method validation experiments w.r.t ICH guidelines, like specificity, linearity, Limit of Detection, Limit of Quantification, method precision and accuracy (recovery) parameters with allowable TTC limit. The achieved limit of detection (LOD) values was 3 µg/g. This developed method was found to be linear with correlation coefficient is greater than 0.999. Developmental and validation experiments were discussed in detail in this...
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Posted by admin on Sep 30, 2020 in |
The overproduction of oxidants in the body leading to different side effects and chronic diseases, including oxidative stress. The oxidative stress is the overproduction of the oxidant’s condition in the body, which leads to metabolic imbalance and leading to diseases. It is one of the main diseases around the world, affecting the human lifestyle, either psychologically and physically. The natural products have been using as medicines and food supplements because of their nutritional values and less side effects. The current study was aimed to evaluate the free radical scavenging activity of Elytraria acaulis root extracts on different free radicals. The anti-oxidant activity was studied against 2, 2-diphenyl-1-picrylhydrazyl (DPPH), superoxide, and hydroxyl free radicals using standard methods. The Elyraria acaulis root part extracts showed concentration-dependent antioxidant activity on free radicals. The extracts showed a moderate effect on free radicals compared to the standard drug ascorbic acid, and hydro-alcoholic extract possesses more activity than the other two extracts. The end results of the current study clearly state that Elytraria acaulis root...
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Posted by admin on Aug 31, 2020 in |
The objective was to develop and validate an easy, economical, fast, reliable, reproducible, precise and accurate reversed-phase high-performance liquid chromatography (RP-HPLC) method for the estimation of tapentadol hydrochloride (TAP, a mu opioid-receptor agonist, and noradrenaline reuptake inhibitor) in the bulk and pharmaceutical dosage form. The chromatographic separation was achieved by using the HPLC system equipped with a C-18(2) column (250 × 4.6 mm, Particle size 5µm) at wavelength 217 nm. The mobile phase consisting of Potassium phosphate buffer: Acetonitrile (50:50 v/v) was used in isocratic mode. The flow rate was fixed at 1.0 mL/min with a continuous run up to 5 min, while the retention time was located near about 2.5 min. In the concentration range of 60-160 µgmL-1, the detector response was found linear with linear regressed equation Y=22791X+120122. In the assay of TAP, 99.65% of the drug was recovered. This method proved a satisfactory validation for all the parameters such as accuracy, linearity, specificity, precision, range, ruggedness, robustness, reproducibility, and peak purity assessment as per ICH guidelines. The results...
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Posted by admin on Aug 31, 2020 in |
Kutki (Picrorrhiza kurroa) is a medicinal herb with remarkable pharmacological properties. However, poor solubility of the active principles limits its medicinal value. This study sought to prepare kutki phospholipid complex in phospholipids such as phosphatidyl choline in order to improve its solubility and permeability. Kutki phospholipid complex (phytosome) was prepared by solvent evaporation method. The study was conducted using central composite design (CCD) with a 33 designs and total of 20 experimental runs. The complex was characterized for solubility, entrapment efficiency, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), particle size and zeta potential, Differential scanning calorimetry (DSC), X-ray diffraction (XRD), drug release, and in-vitro antioxidant activity. The optimized batch showed substantial enhancement of solubility and entrapment efficiency. FTIR study indicates the interaction between phosphatidylcholine and Kutki extract. SEM showed the surface morphology of complex depicting the conversion of crystalline drug into amorphous complex. The in-vitro release was significantly higher for the complex (93.43%) than the drug (19.65%) after 7 h. Enhanced solubility, absorption and antioxidant effect may...
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