Posted by admin on Jun 30, 2020 in |
The aim of this study was to analyze the molecular weight of protein and in-vitro antioxidant activity of marine gastropods Turbo brunneus, Cypraea annulus, and Babylonia spirata. The molecular weight of the protein was determined using SDS-PAGE, and its antioxidant potential was carried out by DPPH radical scavenging activity, reducing power activity and H2O2 radical scavenging activity. The molecular weight of protein varies from 54 kDa in T. brunneus, 38 kDa to 60 kDa in C. annulus, and 44 kDa to 116 kDa in B. spirata. The DPPH scavenging effect was high in Cypraea annulus (78.30%) followed by Babylonia spirata (65.20%) and Turbo brunneus (64.89%). C. annulus showed the highest reducing power of 95.36% at 500 µg/ml concentrations and lowest reducing the power of 52.07% at 100 µg/ml concentrations in the T. brunneus. The maximum radical scavenging activity was reported in T. brunneus (71% at 150 µg/ml concentrations), and minimum activity was reported in C. annulus (8.34% at 50 µg/ml concentration). The results show that tissue extracts of three...
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Posted by admin on Jun 30, 2020 in |
The objective of current research work is exploring the use of natural mucilage for sustained release characteristics for the formulation of microspheres. Orifice ionic gelation method was used to formulate the microspheres by using complex-forming polymer sodium alginate in combination with isolated mucilage. Mucilage from Colocacia esculenta corms were isolated and combined with sodium alginate to fabricate in the form of microspheres. Resultant batches were characterized by differential scanning calorimetry, Fourier transforms infrared spectroscopy, scanning electron microscopy, X-ray diffractometry, swelling capacity, flow properties, particle size, and in-vitro dissolution behavior. Isolated mucilage was found to be swellable in water and amorphous in nature. FTIR and DSC study indicates compatibility between drug and selected polymer. All the formulations exhibit better flow properties. Particle size was found in the range of 780-880 micron. The optimized formulation is releasing the drug for the period of 12 h. Colocacia esculenta corms mucilage along with sodium alginate can be efficiently utilized to retard the drug release and minimize the side effects of the drug, so...
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Posted by admin on Jun 30, 2020 in |
Pseudomonas aeruginosa is one of the most emerging multidrug-resistant opportunistic pathogens, which poses a threat to immunocompromised patients. Treatment of P. aeruginosa in the affected patients has become difficult because of its ability to develop resistant strains against most of the conventional therapeutic antibiotics. Quorum sensing plays a crucial role in the virulence nature of P. aeruginosa as it helps the bacteria to produce virulence factors and biofilm formation. In the present study, we studied the quorum quenching potential of fungal secondary metabolites from the well-known fungus Phoma multirostrata PUTY3 strain isolated from Carica papaya. In this study, we have carried out both in-vitro and in-silico analyses of fungal secondary metabolites from P. multrirostrata against P. aeruginasa. The in-vitro analysis showed a marked decrease in the production of virulence factors such as pyocyanin, rhamnolipids, protease, elastase, exopolysaccharides, HCN gas production and biofilm formation. In addition to this, a significant inhibition in the motility factors of P. aeruginosa such as swimming, swarming, twitching was also observed. In-silico studies further validated...
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Posted by admin on Jun 30, 2020 in |
The objective of the present study is to evaluate phytochemical composition and HPTLC finger print profile of medicinally useful plant Martynia annua L. (Martyniaceae) root extracts. The CAMAG HPTLC system was used for the finger print profiling of root extract using the mobile phase chloroform: Methanol (9:1 v/v). The profile showed that the root extract of Martynia annua exhibited several peaks with different Rf values when visualized at 254 nm and 366 nm. At 254 nm a total 7 peaks were observed and at 366 nm 9 peaks were observed. The result of HPTLC analysis of Martynia annua root extracts shows that the maximum number of chemical constituents present in alcoholic root extract of Martynia annua in comparison to hydro-alcoholic and aqueous root extract of Martynia annua in given solvent system of chloroform and methanol. Further bioactivity guided fractionation and analysis of isolated chemical entity can reveal the active constituents in the different root extract of Martynia...
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Posted by admin on Jun 30, 2020 in |
The objective of the work was to formulate a self-nanoemulsifying drug delivery system (SNEDDS) of zaltoprofen and to study the impact and interaction of various formulation variables on the formulation by the design of the experiment. A 13-run 3-factors, 3-levels box behnken design (BBD) was used for optimization with 1 replicates at the center point. The concentration of phosal 53 MCT, tween 80, and PEG 400 as oil, surfactant, and co-surfactant, respectively, were used as independent variables. Self emulsification time, % transmittance, and relative turbidity were chosen as responses for the study. Results obtained show the impact of independent variables on the response under study. The optimal concentrations of variables were found to be 27.71% (w/w) oil, 46.1% (w/w) surfactant and 19.28% (w/w) co-surfactant. The in vitro dissolution study showed a marked increase in dissolution rate than zaltoprofen powder. The box Behnken design was successfully used in the optimization of zaltoprofen SNEDDS and to study the impact of components on the response under study in a simplified and time-effective...
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