Posted by admin on Jun 30, 2020 in |
Objective: Many studies have demonstrated the antioxidant properties of extracts of Coleus aromaticus. The purified Coleus aromaticus leaves protein was studied to investigate its in-vitro antioxidant activity, preventing oxidative DNA damage induced by hydrogen peroxide (H2O2) and its protective effect on lymphocyte cell viability. Materials and Methods: In-vitro antioxidant capacity by DPPH assay, hydrogen peroxide (H2O2) used to damage the calf thymus DNA, the purified Coleus aromaticus leaves protein (CALP) at 25 μg concentration was used to prevent the H2O2 induced DNA damage, which was demonstrated on submarine agarose gel electrophoresis. The protective effect of purified CALP on cell viability of human peripheral lymphocytes against damage induced by H2O2 was studied by the Trypan dye blue exclusion method. Standard antioxidants like gallic acid, ascorbic acid, and butylated hydroxyanisole were used. Results: The purified CALP protein showed significant antioxidant activity when compared to Gallic acid. At 25 μg concentration of purified CALP provided almost equal protection as given by standard antioxidant Ascorbic acid at 400 μM concentrations against H2O2 induced...
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Posted by admin on Jun 30, 2020 in |
Objective: Kojic acid and arbutin are potent agents for effective treatment of hyperpigmentation. However, low photostability, low permeability and high irritancy exhibited by them make topical delivery a challenge. The aim of this study was to develop novel photostable and highly penetrable formulation of kojic acid and arbutin to combat hyper-pigmentation effectively at lower concentrations in comparison to marketed conventional formulations. Methods: Kojic acid and arbutin loaded micro-emulsion based crème’ gel was formulated by the generation of water in oil system by spontaneous formation technique followed by incorporation into a gel. Thus manufactured by simple industry feasible technique and evaluated for effective reduction of skin irritancy, improvement in photostability, the penetrability of the hydrophilic drugs and anti-tyrosinase potential. Results: Comparative in-vitro and ex-vivo release study showed that microemulsion based crème’ gel not only provided sustained effect up to 24 h but also retained approximately 10 folds higher drug concentration of drugs in the skin as against conventional gel formulation. This novel formulation was not associated with any irritation potential...
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Posted by admin on Jun 30, 2020 in |
A study has been undertaken with an aim to isolate potent endophytic fungi from mangrove plant Lumnitzera racemosa Linn. Among the 10 different fungal strains isolated, one potent strain with broad-spectrum antagonistic activity was found. Basing on the morphological, cultural and molecular characteristics, the potent fungal strain was identified as Cladosporium cladosporioides. Production of bioactive metabolites by the strain was high in Malt Extract Broth compared to other media tested. The culture utilized Mannitol and Beef extract as good carbon and Nitrogen sources for the elaboration of bioactive metabolites. The optimum pH and Temperature for bioactive metabolite production of the strain were recorded at 4.0 and 30 °C. The secondary metabolites produced by the strain grown under optimal conditions exhibited high antagonistic activity against gram-positive as well as Gram-negative bacteria and fungi. This is the first report of Cladosporium cladosporioides from mangrove plant Lumnitzera racemosa...
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Posted by admin on Jun 30, 2020 in |
The main objective of this research work was to check the presence or absence of the phytochemical chemicals in all the selected medicinal plant. Phytochemical analysis of these plants confirm the presence of various phytochemicals like saponins, tannins, amino acids, phenols, carbohydrates, proteins, flavonoids, steroids, alkaloids and glycosides. A. catechu extract are analyzed by using TLC with varying solvent systems. It was another test Column chromatography, Chloroform: methanol (2:8) gave three and six fractions. In FTIR, the functional groups such as 1º, 2º amines, amides, carboxylic acids, alkanes, 1º amines, nitro compounds, aliphatic amines, and alkyl halides and searched the identity of secondary metabolites, which may act as 3,7-Diacetyl-1,5-Dimethyl-3,7-Di azabicyclo- [3,3,1] Nonan-9-One compound and clearly indicates that phytochemical. Chemical constituents of eleven compounds were identified in the methanol extract. The major components were 3, 7-Diacetyl-1, 5-Dimethyl-3, 7-Di azabicyclo- [3, 3, 1] Nonan-9-One (27.80 and C13H20O3N2). The results were obtained suggest that, in addition to their pharmaceutical and medicine sources of 3, 7-Diacetyl-1, 5-Dimethyl-3, 7-Di azabicyclo- [3, 3, 1] Nonan-9-One...
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Posted by admin on May 31, 2020 in |
Eplerenone is a poorly soluble drug that belongs to class II biopharmaceutical classification system. It is a selective aldosterone receptor antagonist, which binds to the mineralocorticoid receptor and blocks the binding of aldosterone, thereby decreases the sodium resorption and subsequently increasing water outflow. This leads to a decrease in blood pressure and used in congestive heart failure. The aim of the present study was to improve the solubility, dissolution and permeability properties of Eplerenone by liquisolid compacts, thereby enhancing oral bioavailability. Different formulations of Liquisolid were developed by dissolving the drug in mixture of Transcutol HP: Capmul MCM and Cremophor EL: Capmul MCM (Non-volatile liquid; 1:1 ratio), converting this liquid medication using carriers as fujicalin and neusilin and coating material as syloid FP 244. The results showed that Liquisolid formulation exhibited significantly higher drug dissolution rate compared to pure drug as well as marketed formulation. The plasma concentration-time profile of healthy Wister rats indicated that the oral bioavailability of optimized formulation has been significantly improved compared to pure drug...
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