Posted by admin on Mar 31, 2020 in |
The purpose of current research work is to evaluate the in-vitro antioxidant and in-vivo hepatoprotective activity of Ganoderma lucidum strains DARL-4 and MS-1. Antioxidant activity of different extracts was evaluated by using DPPH and ABTS method. Hepatoprotective activity of hydroalcoholic extract of G. lucidum strain DARL-4 and MS-1 were evaluated by paracetamol-induced liver damage in rats. The hydroalcoholic extract of DARL-4 and MS-1 were orally administered to the animals at a dose of 300 mg/kg once daily for 14 days. On the 14th day the paracetamol (2 g/kg) was also orally administered. The degree of protection was measured by estimating the serum biochemical parameters. The result showed that the hydroalcoholic extract of DARL-4 and MS-1 showed potent antioxidant activity than the ethanolic, methanolic and aqueous extract. The serum biochemical parameters which were elevated due to paracetamol toxicity were significantly reduced. The histopathological studies also supported that extracts markedly reduced toxicity of paracetamol. The hydroalcoholic extract of MS-1 showed a more potent hepatoprotective effect than that of DARL-4. The present...
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Posted by admin on Mar 31, 2020 in |
Formulation development for new drugs, an extension of formulations, finding an alternative to conventional dosage forms for better therapeutic response are some of the research impetus in Pharmaceutical F and D. The new -untried routes of administration are also an avenue that can be explored. Under this research zolmitriptan, an antimigraine, BCS class II drug is formulated into mucoadhesive films. Zolmitriptan shows 40 to 50% oral bioavailability owing to its low solubility and degradation in GIT. Mucoadhesive films can be an effective formulation for this drug owing to its good permeability. Direct absorption in blood and preventing GIT degradation by mucoadhesion on buccal mucosa can be two rationales stated for the improvement of bioavailability of zolmitriptan via mucoadhesive films. Migraine strikes as abrupt and disturbing health situation and needs immediate treatment. Thus, the mucoadhesive film can be more promising than oral tablets for zolmitriptan in the treatment of migraine. Blank mucoadhesive films were prepared using various combinations of. HPMC K15, Eudragit L 100, whereas concentrations of chitosan, PVA, beta-cyclodextrin,...
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Posted by admin on Mar 31, 2020 in |
The emerging need for the multidrug regimen of M. tuberculosis strains urged us to synthesize novice and potent anti-tubercular medicaments. Our interest in this work had afforded a series of novice cinnolines as a new structural category of antituberculosis agents. All 14 compounds were synthesized using the appropriate scheme, two-step reactions with the high yielding product. The newly synthesized cinnoline Compounds were examined for their in-vitro drug-sensitive M. tuberculosis H37Hv strain. To date, evaluation of anti-tubercular activity various drugs in the multidrug regimen is on going out of which INH highly effective component drug and is advised by WHO. Some of the derivatives were considered to be promising inhibitors of M. tuberculosis. For example, the most active compound (CN-14) exhibited 12.5 μg/ml inhibitions against drug-sensitive M. tuberculosis H37Rv...
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Posted by admin on Mar 31, 2020 in |
Obesity ailments, commonly known as “New World Syndrome,” has drawn the attention of the scientific community to explore novel natural product-based therapeutics as effective alternatives. In this study, different solvent extracts of Moringa oleifera (M. oleifera; Drumstick tree) leaves were prepared, and their phytochemical analysis was done. The therapeutic effect of M. oleifera leaf extracts on key enzymes related to obesity and against oxidative stress was studied through standard assays (DPPH, ABTS, NO, FRAP). Among different solvent extracts, the M. oleifera leaf hydroalcoholic extract (MOHE) contained a high amount of polyphenols (135.45 ± 3.435 μg/mL) and flavonoids (129.52 ± 3.102 μg/mL). The IC50 values of MOHE for α-glucosidase, α-amylase, and pancreatic lipase were 373.6 μg/mL, 339.8 μg/mL and 437.1 μg/mL respectively which were much lower than rest of the M. oleifera extracts. The GC-MS analysis of MOHE showed the presence of many glycosides, oils and polyphenolic compounds, as mentioned in the results section. In conclusion, our data suggest that M. oleifera leaves are rich in several bioactive phytochemicals that qualify it as an...
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Posted by admin on Mar 31, 2020 in |
Introduction: The present study designed to investigate Human parvovirus B19 (HPV-B19) as an etiological agent of rash–fever ill patients confirmed negative for measles and rubella infection which is responsible for the same symptoms in North Indian population. Materials and Methods: Total 319, negative serum samples selected after measles/rubella IgM antibodies detection. The patients between the age of (4 to 52 years) with fever and rash-like illness were divided into four groups: 4-14, 15-25, 26-36 and >37 years old. The serological analysis was done by Indirect IgM ELISA and Real-time PCR used for molecular identification of HPV-B19 in this study. Results: The prevalence of HPV-B19 was found to be 29.4% by both methods in Measles and Rubella negative serum samples. We found a 29.4% positive result by HPV-B19 IgM ELISA and 7.5% positive by Real-time PCR against HPV-B19 infection. The results have been statistically significant (p= 0.0001*). The highest prevalence (38.5%) was found between 15-25 years of age group. Conclusion: The study was designed to find the frequency of HPV-B19...
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