Posted by admin on May 31, 2020 in |
The aim of the present work is to determine the feasibility of peracetic acid (PAA) and the coexistent hydrogen peroxide (HP) simultaneous quantitative determination in disinfectant solution by voltammetry using carbositall rotation electrode (CE) as indicating electrode. It has been experimentally proved that the optimum pH for analysis is approximately 3.6. The linear relationship has been observed in the PAA concentration range (3.12–12.50)´10–5 mol L–1, the calibration curve equation was Iр = (3.78 ± 0.46)´103с (r = 0.995); in the HP concentration range of (0.94–3.76)´10–4 mol L–1, the calibration curve equation was Iр = (3.57 ± 0.26)´103с + (0.11 ± 0.07) (r = 0.998). Determining PAA in the working solution with the concentrations of 6.24´10–5, 7.80´10–5 and 9.36´10–5 mol L–1 the RSDs were 0.035, 0.028 and 0.022 respectively (δ = +0.30…+0.90%), determining HP in the working solution with the concentrations of 1.88´10–4, 2.35´10–4 and 2.82´10–4 mol L–1 the RSDs were 0.028, 0.018 and 0.011 respectively (δ = –0.77…+0.92%). Determining PAA in the test solution of “Delakson” disinfectant with the concentrations of 0.1%, the RSD...
Read More
Posted by admin on May 31, 2020 in |
Present work was aimed to determine, identify, and characterize the bioactive chemical compounds from methanolic leaves extract of Abutilon fruticosum by GC-MS analysis. Fresh disease-free leaves were collected shade dried and powdered for extraction with HPLC grade methanol. Preliminary phytochemical screening of methanolic leaves extract was performed using standard methods to determine the presence of different chemical compounds; the crude extract was subjected to GC- MS analysis for the identification of bioactive compounds. Phytochemical screening of methanolic leaves extracts revealed the presence of carbohydrates, proteins, alkaloids, phenols, flavonoids, terpenes, phytosterols, etc. Furthermore, GC MS analysis of the extract revealed the presence of 65 bioactive compounds. Some major biologically active compounds identified were Azulene (24.91%), Hexadecanoic acid (13.27%), Phytol (9.51%), Beta-sitosterol (3.19%), Lupeol (1.21%), Campesterol (0.33%) etc. These chemical compounds are biologically active and pharmacologically important. The study provides detailed information about the identification and chemical characterization of various medicinally important phytocompounds from methanolic leaves extract of this plant. Although the plant is rare and endemic to Indian Thar Desert...
Read More
Posted by admin on May 31, 2020 in |
Substituted triazoles have received considerable attention during the last two decades as they are endowed with a variety of biological activities and have a wide range of therapeutic properties. The present work is concerned with the synthesis of fused 1, 2, 4-triazole derivatives with the objective of discovering novel and potent anti-tubercular agents that might be devoid of harmful side effects. 1-[amino (isonicotinoyl) carbonohydrazonoyl] guanidine (I) was prepared from isoniazide and cyanoguanidine and was cyclized to 2-(3-pyridin-4-yl-1H-1,2,4-triazol-5-yl) guanidine (II) which was further converted into amino triazolo triazine derivatives (IIIa-g) by refluxing with the appropriately substituted benzaldehydes. The final compounds 10-substituted-2-(pyridin-4-yl)-6,10-dihydro-5H-pyrimido[2,d] [1,2,4] triazolo[1,5-a] [1,3,5] triazine-6-carboxylic acid (IVa-g) were obtained by refluxing different amino triazolo triazine derivatives (IIIa-g) with formaldehyde and sodium pyruvate in presence of HCl in methanolic medium. The newly synthesized compounds were screened for their antitubercular activity using a microplate alamar blue assay method and characterized on the basis of IR, 1HNMR and Mass spectral...
Read More
Posted by admin on May 31, 2020 in |
In folk medicine, especially in developing countries, several plant species are employed for the treatment of snakebites that lack prompt access to serum therapy. Andrographis serpyllifolia (Vahl), belonging to Acanthaceae, has been recorded in ethnobotanical archives as a plant possessing potent anti-ophidic activity for snake bites. The present study emphasis to investigate the phytochemical profile and anti-oxidant properties of various plant extracts of A. serpyllifolia. To carry out this work, various cold plant extracts were prepared from shade dried leaves and used. During qualitative analysis, all the plant extracts showed positive results for all phytocompounds. Various quantitative assays exhibited that all plant extracts were having significant level of secondary metabolites with values ranging from 1148 to 286 mg GAE/g for phenol, 1262 to 976 mg LN/g for terpenoids, 172 to 106 mg RE/g for flavonoids and 21.5 to 3.6 mg TA/g for tannins. All the plant extracts tested had higher DPPH, ABTS scavenging activity, and metal chelating ability. The free radical scavenging activity of plant extracts were found to...
Read More
Posted by admin on May 31, 2020 in |
Nitrofurantoin is a broad-spectrum bactericidal antibiotic that affects both gram-negative and gram-positive bacteria. Nitrofurantoin exhibits bacteriostatic or bactericidal effects by inhibiting the synthesis of DNA, RNA, protein, and cell wall synthesis. Nanocrystals of NFT were prepared by a cold high-pressure homogenization technique. To improve the dissolution rate, the drug was formulated in a nanocrystal by using an emulsion diffusion method, high-pressure homo-genization or sonication. NFT was dispersed in aqueous surfactant solution containing poloxamer 188, PVPK 30, and HPMC E3 under continuous stirring. Poloxamer 188 was used as a surfactant for the preparation of the NCs. Formulation NC9B3 has a mean particle size 231 ± 9 nm with polydispersity index 0.09 ± 0.02, which indicates a very narrow particle size distribution. % Entrapment efficiency was 98.3 ± 0.7 slow drug release profile indicates the homogeneous dispersion of NFT in the lipid matrix. NCs have crystalline nature with rough surfaces, which has been confirmed using SEM analysis. XRPD spectra show the reduction in the crystalline behavior of the drug and the...
Read More
