Posted by admin on Mar 31, 2020 in |
Complementary and alternative medicines (CAMs), particularly herbal medicines (HMs), are popular with diabetic patients and are often used alongside conventional treatments. In such cases, patients usually do not advise their doctor or any other healthcare professional, so inappropriate use and potential interactions between CAM therapies and conventional treatments are serious concerns. This study attempted to identify the main factors affecting CAM decision-making by studying the association between CAM use and patient characteristics, including age, gender, diagnosis, reasons for using, time of starting, and sources of HM products. It was aimed to shed light on patients’ experiences and perceptions of CAM use, particularly HMs, to identify any perceived benefit or harm. Cross-sectional questionnaire surveys, including open-ended and closed-ended questions, which were administered to eligible participants. The results revealed that approximately 34% of the 98 patients participating in the study used CAM. The most prevalent age group of participants was 51-60 years old (39.8%). The most used HMs were the olive leaf (21.2%), green tea (21.2%), cinnamon (21.2%), black seeds (15.2%),...
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Posted by admin on Mar 31, 2020 in |
The objective of the present study was characterization, production, and purification of Nattokinase from Bacillus subtilis. A fibrinolytic enzyme-producing bacterium was isolated and identified as Bacillus subtilis from fermented soybean and soil. Bacillus subtilis showed a very strong protease activity by degrading casein skimmed milk agar. Nattokinase was produced on basal medium and then purified from supernatant. The crude enzyme was purified by ammonium sulphate precipitation, followed by dialysis and DEAE cellulose ion-exchange chromatography. Total protein was estimated by Lowry’s method (0.58 mg/ml for FS2 and 0.49 mg/ml for S1) and enzyme-specific activity was determined by tyrosine standard (0.52 µmole/mg/ml for S1 and 0.59 µmole/mg/ml for FS2). The molecular weight of purified enzyme was determined by SDS-PAGE (20-24 kDa for FS2 and 41-44 kDa for S1). Finally, the fibrinolytic activity was determined by application of extracted enzyme on pre-existing human blood clots (FS2 showed greater activity compared to...
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Posted by admin on Mar 31, 2020 in |
Environment provides a solid platform for good health. An unhealthy environment makes place unsafe for wild animals. One of the effects is a parasitic load on these animals, as a high degree of parasitization may cause harm to the health of these animals. In the present research work, blood parasitic infestation in two species of lizards, Hemidactylus flaviviridis and Calotes versicolor were investigated and it was observed that lizards from the highly polluted area were infested with high parasite load that ultimately caused harm to these animals which biologically constitute an important part of the food chain. The intensity of blood parasites (Hepatozoan and Plasmodium) in lizards from different habitats was studied and it was observed that highly polluted areas (Mirzapur) had a high incidence of the parasites. It is known that Mirzapur is more polluted than Bareilly and Chandausi, the other sites selected for investigation. Parasites are regarded as indicators of pollution. To make the environment healthy and peaceful, positive energy and healthy habitats can contribute to a...
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Posted by admin on Mar 31, 2020 in |
The present study aimed to investigate the combined anticonvulsant effect of nifedipine (calcium channel blocker) and pentazocine (opioid analgesic) on the duration of convulsions, tonic hind limb extension and recovery in mice and rats. The study was initiated after obtaining ethical approval from the Institutional Animal Ethics Committee (IAEC), Department of Pharmacology, Osmania Medical College, Koti, Hyderabad. The anticonvulsant effect of these drugs were screened using Maximal Electro-Shock (MES) method and animals showing tonic hind limb extension response were divided into groups (six animals per group) in both species. Both mice and rats (6 animals/group) were treated with nifedipine (10 mg/kg), pentazocine (30 mg/kg), a combination of nifedipine (10 mg/kg) and pentazocine (30 mg/kg) and control animals are given distilled water as vehicle. The drug administered by an intraperitoneal route. The data were analyzed using ANOVA and group means were compared with LSD Post Hoc Test. P values < 0.05 were considered as significant. The animals treated with both nifedipine 10 mg/kg and pentazocine 30 mg/kg showed a significant...
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Posted by admin on Mar 31, 2020 in |
The main of this study was production of floating tablets to maintain the drug stay in its absorption area. Ciprofloxacin is better absorbed in the stomach because of this reason floating matrix tablet was prepared using novel sintering technique with sodium bicarbonate as gas generating agent and a polymer eudragit robust formulation was decided on the basis of floating time and in-vitro drug release with possible less polymer concentration, time of exposure and at low temperature. Three batches were prepared with hardness between 4-6 kg/cm2 based on time of exposure at particular sintering temperature desired total floating time, and drug release was retarded up to 12 h. Hence, it is evident from this investigation that a floating matrix tablet using the sintering technique could be promising delivery with improved sustained release action and drug...
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