Posted by admin on May 31, 2020 in |
Stability indicating the HPTLC method was developed for simultaneous estimation of teneligliptin and metformin by using aluminum plates precoated with silica gel 60 F224 (Merck) and mobile phase toluene: methanol: GAA: TEA (5:4:0.5:0.5) with scanning wavelength of 257 nm. The developed method was validated for linearity, stability, accuracy, precision, robustness, LOD and LOQ as per ICH guidelines. The proposed method was successfully applied for the assay of metformin and teneligliptin in dosage forms and results were found to be in an acceptable range with a labeled claim of the pharmaceutical dosage form. Linearity for metformin and teneligliptin was observed\with correlation coefficient (r2) value of 0.999 and 0.993. The proposed method was also applied for degradation study of dosage form in alkaline acidic, Thermal, and photolytic conditions. Further, kinetic degradation study in alkaline conditions (0.1 N, 0.5 N, 1.0 N NaOH) at 60 °C and 80 °C are carried out. The degradation rate constant was also predicted at different temperatures and strengths of NaOH using kinetic methods. Degraded product of...
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Posted by admin on May 31, 2020 in |
The present study was designed to evaluate the in vivo effect of withacoagulin and coagulin C isolated from Withania coagulans Dunal fruits on blood pressure of albino rats. DOCA (10 mg/kg; i.p.) along with diet high in sodium chloride salt was used to induce hypertension in rats. Comparative effects of withacoagulin and coagulin C isolated from Withania coagulans Dunal fruits were observed. Female Albino rats (100-120 g) were divided into seven experimental groups (n = 6). Normal control, Verapamil standard control (50 mg/kg, p.o.), withacoagulin treatment groups (25, 50 mg/kg; p.o.), coagulin C treatment groups (25, 50 mg/kg; p.o.) and Toxic control (DOCA with 2% normal saline ad libitum). Rats having more than 150 mm Hg systolic blood pressure were chosen for the treatment. Noninvasive tail blood pressure amplifier (NIBP200A) was used to measure the blood pressure of conscious rats at a fixed interval of time every day for four weeks. A significant decrease (P< 0.05) in the blood pressure of withacoagulin treated and the standard group was observed....
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Posted by admin on May 31, 2020 in |
Antioxidants are the substance having the potential to quench free radicals and significantly delay or inhibit oxidation of the substrate, thus protect biological systems against potentially harmful effects of free radicals; in low concentrations. The present study was aimed to evaluate the in-vitro antioxidant activity of Oldenlandia corymbosa L., a locally available medicinal plant of Assam, used as vegetable in diet and traditional medicine for liver disease and jaundice. The dried powdered plant material was extracted with 80% ethanol by Soxhlet extraction and was used for the fractionation in different solvents (methanol, petroleum ether, and ethyl acetate). The fractionated extracts were subjected to the analysis of in-vitro antioxidant activity with DPPH (2,2, Diphenyl-2-picryl hydrazyl), hydroxyl radical, nitric oxide, reducing power, and phosphomolybdenum assays for total antioxidant capacity. Ascorbic acid was used as standard at concentrations (20 μg/ml, 40 μg/ml, 60 μg/ml, 80 μg/ml and 100 μg/ml) dissolved in ethanol. Samples were prepared in the same manner. Results from in-vitro experiments revealed the significantly (p<0.05) high antioxidant activity in hydro-ethanolic...
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Posted by admin on May 31, 2020 in |
Background: Lafutidine floating beads as a gastro-retentive dosage form antagonisms of histamine H2 receptor. It is used as anti-ulcerative agent. It is effective against the oesophageal lesions induced by acid reflux through inhibition of acid secretions. Objectives: The objective of the present work was to formulate and evaluate the floating beads of Lafutidine as a model drug. The objective of this study is to develop a simple uncomplicated and easy to manufacture floating beads that are capable of delivering lafutidine at a prolonged release rate of delivery. It has a short half-life (3 h). It has a low bioavailability (60-80%). The frequent dosing, which results in unacceptable patient compliance. Methods: Lafutidine was received as a gift sample and a thorough pre-formulation study was performed on a given sample in order to estimate the physicochemical properties like solubility, melting point, partition coefficient to confirm the authenticity of sample and to confirm that there are no significant barriers to the development of dosage forms. 12 different formulation of lafutidine floating alginate...
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Posted by admin on May 31, 2020 in |
The objective of the method is to develop a rapid, simple, accurate, precise RP-HPLC method for the simultaneous estimation of Ivabradine and Metoprolol in tablet dosage form. The analytes were run through BDS C18 150 × 46 mm, 5µm column, and detected at a wavelength of 260 nm using PDA 996 (photodiode array) detector. Mobile phase containing buffer 0.01N disodium hydrogen phosphate (3.5±1 pH) and acetonitrile in the ratio of 65:35 v/v was pumped through the column at a flow rate of 1.0ml/min and run time is about 6 min. The temperature was maintained at 30 °C. Retention times of ivabradine and metoprolol were found to be 2.38 min and 3.53 min. Percentage RSD of ivabradine and metoprolol was found to be 0.4 and 0.7, respectively. Percentage recovery of ivabradine and metoprolol were found to be 99.80% and 100.11%. LOD and LOQ values obtained from regression equations of ivabradine and metoprolol were found to be 0.03, 0.08, and 0.26, 0.79, respectively. The degradation studies were performed by applying different...
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