Posted by admin on Jul 31, 2020 in |
Notable progress has been made in methods that encourage the use of PCR as a rapid and accurate tool in quality evaluation of pharmaceuticals. In this study, monoplex and multiplex PCR based assays were developed and compared with standard conventional methods for rapid detection of three specified topical indicator pathogens, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans, in nonsterile pharmaceutical preparations. The detection limit of monoplex PCR assays for the microbial targets was achieved at 100 fg purified DNA and 10 CFU/ml for Pseudomonas aeruginosa and 1fg purified DNA and 10 CFU/ml for each of Staphylococcus aureus and Candida albicans. No change in the detection limit for cfu/ml of the three tested indicator pathogens was obtained upon using mPCR assays. The results of applying both conventional and PCR detection methods for different cream and lotion preparations revealed a 100% correlation between both methods. The PCR based detection method can be completed in 8 h versus 5-6 days in case of conventional methods, but the former can’t differentiate between viable...
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Posted by admin on Jul 31, 2020 in |
Antimicrobial drug resistance is one of the major threats due to the wide usage of broad-spectrum antimicrobial drugs in the general population. The present study was undertaken to investigate the antibacterial potential of Alternanthera philoxeroides methanolic extract (APME) against multidrug-resistant UTI pathogens. The preliminary phytochemical screening of the extract showed the presence of carbohydrates, amino acids, proteins, alkaloids, glycosides, steroids, flavonoids, tannins, and phenolic compounds. The quantitative estimation of total phenolic, tannin, and flavonoid contents was determined for the crude extract of APME through standard curves of gallic acid and rutin. The phenolic content of APME was found to be 12.4 mg of gallic acid equivalent (GAE)/g of extract. The tannin content was found to be 5.6 mg of GAE/g of extract, whereas the flavonoid content was found to be 3.2 mg of rutin/g of extract. The antibacterial potential of the extract was observed as maximum against Staphylococcus saprophyticus, moderate against Escherichia coli and Proteus vulgaris, and minimum against Proteus mirabilis. The minimum inhibitory concentration was ranged from 12.5...
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Posted by admin on Jul 31, 2020 in |
Liver diseases are a worldwide problem. Conventional drugs used in the treatment of liver diseases are sometimes inadequate and can have serious adverse effects like mild digestive problem, headache, nausea etc. It is necessary to search for alternative drugs and for treatment of liver disease to replace currently used drugs of doubtful efficacy and safety. Herbal therapies are free from side effects and toxicity, unlike allopathic medicines. Ayurvedic drugs are also acts by multiple pathways and shown full protection in liver disorders. Composition of marketed formulation Livsplin syrup (Test A) and Li- verve tonic (Test B) containing different plant extracts reported hepatoprotective and antioxidant activities. Thus these formulations may be synergistically or additively may show beneficial hepatoprotective activity with allopathic medicines. Hence, it may useful as alternative medicine to cure different kinds of liver diseases in man and animals. From our investigation, results were shown that administration of Li – verve tonic effectively protected against the loss of antioxidant activities of SOD, CAT, MDA respectively compared to control group...
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Posted by admin on Jul 31, 2020 in |
The main purpose of this investigation study is to enhance the solubility and dissolution rate of poorly water-soluble drug Fluconazole. Fluconazole was selected as the choice of drug as it belongs to BCS class II i.e., drugs having low solubility and high permeability, which hinders in-vivo efficacy of drugs giving poor pharmacokinetic profile and low absorption. Solid dispersions are formed to increase the dissolution rate of a drug and solubility, thereby improving its oral bioavailability. Fluconazole solid dispersions were prepared to enhance the solubility and dissolution rate using the Kneading method and Solvent Evaporation method. Solid dispersions were formulated using different carriers like PEG, PVP, Gelucire, etc. The batch containing PEG 4000 as a carrier by using the Solvent Evaporation method gave maximum drug content, and solubility was selected as the optimized batch for the formulation of an oral tablet. The optimized batch was subjected for Practical yield, Drug content, Saturation solubility, Infrared (I.R.) Spectroscopy, Differential Scanning Calorimetry (DSC), X-ray powder Diffractometry, and Scanning electron microscopy. Post and pre-compression...
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Posted by admin on Jul 31, 2020 in |
The ethanolic extract of Moringa oleifera leaves & methanolic extract of rose hips of Rosa damascena were first subjected to various phytochemical tests and further assessed for their antioxidant activity by DPPH method. Different ratio of two drugs in the polyherbal formulation were based on antioxidant activity in albino Wistar rats. Thirty rats (180 -250 g) of either sex were randomly divided into five groups. Group I: Normal Control received 0.9% Normal Saline. Group II: Disease control, received Isotretinoin 60 mg/kg p.o single dose daily for 11 days without any treatment. Group III: Received Isotretinoin 60 mg/kg p.o single dose daily for 11 days and treated with standard drug Imipramine 10-20 mg/kg p.o for the next 45 days. Group IV: Received Isotretinoin 60 mg/kg p.o single dose daily for 11 days and treated with Polyherbal (Moringa leaves & Rosehips) suspension (100 mg/kg p.o.) for next 45 days. Group V: Received Isotretinoin 60 mg/kg p.o single dose daily for 11 days and treated with Polyherbal suspension (200 mg/kg p.o) for...
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