Posted by admin on Feb 29, 2020 in |
The present study was carried out to evaluate phytochemical composition and pharmacological activities of leaf extract of Lusia zeylanica by using different solvents. The solvents like n-hexane, ethyl acetate and methanolic were used to extract dried leaf material of L. zeylanica. These extracts were analyzed for phytochemical constituents with GC-MS analysis and antimicrobial activity tested against four bacterial strains and three fungal strains by using the agar diffusion method. In-vitro anticancer activity was done against two cancer cell lines (MCF-7 and HeLa cell line) by using MTT assay. Phytochemical analysis revealed the presence of coumarins, flavonoids, glycosides, phenols, saponins, tannins, and terpenoids. GC-MS analysis determines the presence of 11 compounds in ethyl acetate, 7 compounds in methanolic extracts and a total of 7 unknown compounds respectively. A significant cancer cell growth inhibition was observed for two extracts with IC50 values ranging between 18.36 µg/ml to 67.914 µg/ml. Our result shows this plant is a promising source of phytocompounds with potential antimicrobial and anticancer...
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Posted by admin on Feb 29, 2020 in |
Acute lymphoblastic leukaemia (ALL) of the B-cell lineage (B-ALL) is a malignant neoplasm identified by the accumulation of immature lymphoid progenitor cells in bone marrow and blood. Numerous chromosomal aberrations have been found to be correlated with disease, relapse and its clinical outcome. The survival and cure rate has been improved drastically with the advent of modern methods of diagnosis and availability of targeted pharmacological agents. However, the limitation is the higher incidence of relapsed disease. An 8-year old male child was diagnosed with B-ALL, the disease relapsed despite proper therapeutic regimen after reporting to the hospital. Conventional cytogenetic analysis revealed a normal male karyotype. FISH panel for B-ALL including t(9;22) BCR-ABL, t(12;21) ETV6-RUNX1, KMT2A (11q23) rearrangement and t(1;19) PBX1-E2A was performed and was positive for translocation t(12;21) ETV6-RUNX1 in 70% of the cells at the time of diagnosis. At the time of relapse, patient underwent cytogenetics workup including cytogenetics and FISH. Bone Marrow karyotyping showed a male karyotype with t(12;21) in all the metaphases studied and FISH was...
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Posted by admin on Feb 29, 2020 in |
Background: Berberine is a natural alkaloid used as traditional Chinese medicine. Objective: The objective of the present study was to evaluate the effect of berberine on dibutyltin dichloride (DBTC) induced acute pancreatitis in albino Wistar rats. Methods: In this study 24 Wistar rats were randomly selected and divided into four groups. Each group consists of 6 animals. Group-1 (-ve control), Group 2 (+ve control or disease control), Group 3 (Berberine 50 mg/kg p.o), Group 4 (Berberine 100 mg/kg, p.o). Acute pancreatitis was induced with dibutyltin dichloride at a single dose of 6mg/kg body weight i.p to all the groups except negative control. At 3rd day, serum amylase and lipase levels were elevated in all groups except negative control meanwhile rats were treated with berberine up to 28 days. The estimation of blood parameters and tissue parameters was carried out by the auto-analyzer. Results: Berberine treated groups significantly reduce elevated serum parameters, and significantly increase the tissue antioxidant parameters to normal. Serum parameters like serum amylase, lipase, fasting blood glucose...
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Posted by admin on Feb 29, 2020 in |
Objective: Nanoliposomes of letrozole a drug used for breast cancer treatment were formulated. The present investigation was aimed to optimize the phospholipids concentration by formulating a series of nanoliposomes formulation and their characterization. Methods: Nanoliposomes were prepared using reverse-phase evaporation technique employing diverse ratios of soya lecithin and cholesterol concentration. The effect of various factors was studied on size, polydispersity index, loading capacity and entrapment efficiency of nanoliposomes. The prepared nanoliposomes were analyzed using DSC and ATR spectroscopy for drug-excipients compatibility. Results: The smallest particle size and polydispersity index (99.45, 0.262) highest entrapment (87.24 ± 0.59) and loading capacity (56.89 ± 0.39) was observed in batch NL8. The disappearance of drug peak in DSC indicates the dissolution of the drug in a polymer matrix. The ATR spectroscopy results show there was no interaction between components and drug is stable in the formulated nanoliposomes. Conclusion: It is concluded that the concentration of soya lecithin and cholesterol were critical parameters that affect the encapsulation of letrozole in the formulation and optimization...
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Posted by admin on Feb 29, 2020 in |
Rheological properties of carboxymethyl locust bean gum (CMLBG) solutions having different concentrations and solution pH were evaluated through steady shear and oscillatory measurements and were used to describe the erosion propensity of CMLBG coat of compression coated placebo tablets. CMLBG solutions, irrespective of concentration and solution pH, exhibited predominant viscous character as the viscous modulus (G”)> elastic modulus (G’) and phase angle (Tan δ) > 1. The viscous contribution was found to be influenced by both the CMLBG concentration and solution pH. An increase in CMLBG concentration increased the pseudo-plasticity and consistency coefficient and decreased the viscous contribution in all solution pH resulting in a decrease in erosion of CMLBG coat. The higher viscous character of CMLBG in buffer solution of pH 7.4 (Solution B) was responsible for higher erosion of the coat than in acid solution of pH 1.2 (Solution A). When the pH of acid solution of CMLBG was increased dynamically from 1.2 to 7.4, the viscous contribution decreased to some extent than that observed in Solution...
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