Posted by admin on Sep 30, 2019 in |
In-silico computational approaches help in ascertaining drug targets via bioinformatics tools. HER2 is the most valuable therapeutic target for breast cancer therapy. The overexpression of HER2 protein plays a very critical role in the progression of breast cancer. Plant-derived natural products have received increasing attention over the past 20-30 years for their potential as novel therapeutic agents. In the future, plant products may offer more effective medicines than synthetic drugs. The main aim of the current study helps to develop new anti-cancer drug candidates from natural and dietary compounds with the help of computational approaches. In this context, the plant-derived compounds resveratrol and its related analogs were taken and docked onto the protein active site (PDB ID:1M14) via structure-based virtual screening for the prediction of novel potential inhibitors, which may be used as anticancer drugs against breast cancer. Furthermore, Molinspiration server and Data warrior software tools were used to evaluate the ADMET profiling and physicochemical parameters of the screened compounds. The best compound was identified, showing good binding affinity...
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Posted by admin on Sep 30, 2019 in |
The present paper portrays a simple, rapid, nonextractive spectrophotometric method for the estimation of an anticholinergic drug, Ipratropium Bromide. The method is based on the formation of an instantaneous stable yellow colored ion pair complex of drug with a chloroformic solution of reagent Bromophenol Blue (BPB) which shows absorption maxima at 416 nm. Job’s plot of continuous variation affirmed the development of 1:1 complex between drug and reagent. The optical parameters were optimized, and the calibration curve was found to be linear in the range of 10 µgmL-1 to 80 µgmL-1. The detection limit was found to be 2.75 μg mL-1 with Sandell’s sensitivity 0.083 μgcm-2. Stability constant (log Kf) was calculated as 6.6. Molar absorptivity was found to be 4.29 × 103 L mol-1 cm-1 with Gibb’s free energy change of – 3.1 × 104 kJ mol-1. The method was validated as per ICH guidelines and had many advantages like low detection limits, high sensitivity and not requiring adjustment of critical conditions, thus paving the way for the...
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Posted by admin on Sep 30, 2019 in |
Rhus pentaphylla (Jacq.) Desf. (Searsia pentaphylla (Jacq.) FA. Barkley) is used for its colorant and tanning properties by the local population. The bark, leaves, roots, and fruits are employed in Moroccan traditional medicine to treat gastrointestinal disorders and diarrhea. Nevertheless, the pharmacological properties of R. pentaphylla on cardiovascular diseases have not yet been presented. This study was carried out to explore the vasorelaxant effect of the aqueous extract (decoction) from the leaves of R. pentaphylla (RpAE), its mechanism of action, and its antihypertensive effect. The results of this study demonstrate that RpAE induces a dose-dependent effect on isolated rat aorta. The vasorelaxation is endothelium-dependent via the muscarinic receptor, Calmodulin/eNOS/sGC/cGMP/PKG signaling pathway through the activation of SERCA pump, the inhibition of VOCC, and opening of KCa2+ channels. In-vivo, RpAE induces antihypertensive effect, ameliorates diuresis, and vascular reactivity on L-NAME induced hypertensive rats. The oral administration of RpAE reveals no mortality or toxicity. This is the first study in Morocco showing vasorelaxant and antihypertensive effects of species belonging to genus...
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Posted by admin on Sep 30, 2019 in |
Natural Herbal components like seeds mucilages, exudates, gums, polysaccharides, celluloses, hemicelluloses, starch, etc. are now a day’s exploited as excipients. Literature reveals medicinal, nutraceutical and pharmaceutical uses of fenugreek seed hemicelluloses in dosage form design. Hemicelluloses like galactomannans are water-soluble polysaccharides highly susceptible to hydrolysis either by acidic conditions or by uncontrolled hydrothermal processes. Chances of breakdown of intact polymeric polysaccharides chains into oligosaccharides, disaccharides, monosaccharide, etc. increases. Conversion into basic sugar units may also take place leading to changes in the polymeric behavior of the intact structures. So, in the present research percentage hemicellulose recovery is determined by invasive, indirect method in terms of basic glucose units. FTIR-SEM-DSC analysis was found to be useful characterization tools to interpret and support the recovery details of the hemicellulose isolates. Finally, it is concluded that the method of isolation of any natural component either hemicellulose, cellulose, etc. targeted to be utilized as excipient entity in dosage form design should be strictly optimized because there are numerous factors in processing which may...
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Posted by admin on Sep 30, 2019 in |
Chemical, physical, and biological methods have been developed to synthesis nanoparticles but chemical and physical methods are involved in the production of toxic by-products which are hazardous moreover the methods are very expensive. To synthesis stable metal nanoparticles with controlled size and shape, there have been searched for inexpensive, safe, and reliable and “green” approach. The present study reports an environmentally friendly, low cost, novel and rapid method for synthesis of silver nanoparticles. We have developed a green synthetic method for silver nanoparticles using Cassia tora leaf extract which acts as a reducing & capping agent. It was observed that use of Cassia tora leaf extract makes rapid and convenient method for synthesis of silver nanoparticles and can reduce silver ions into silver nanoparticles within few seconds of reaction time. UV-Vis spectrometer uses to monitor the reduction of Ag ions and formation of AgNPs in medium. The morphological studies of the biosynthesized nanoparticles are done using UV-Vis, HRTEM & FESEM techniques FTIR was performed to identify the functional groups...
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