Posted by admin on Jan 31, 2019 in |
The present study was carried out to assess the various phytochemical composition, GC-MS analysis, and antimicrobial potential of methanolic leaf extract of Alstonia scholaris (L.) R. Br. by using different solvents. Phytochemical analysis revealed the presence of alkaloids, coumarins, flavonoids, glycosides, phenols, quinines, saponins, tannins, steroids and terpenoids. Susceptibility testing by Agar well diffusion assay showed significant antimicrobial activity with ethyl acetate and methanol extracts of leaves against bacterial strains such as Bacillus subtilis, Bacillus cereus, Pseudomonas aeruiginosa, Staphylococcus aureus, Escherichia coli and fungal strain Candida albicans. The methanolic leaf extract exhibited better antimicrobial activity than ethyl acetate extract. The UV-Vis, FTIR, and GC-MS have been employed to characterize the bioactive compounds present in methanolic leaf extract of Alstonia scholaris. The crude extract was scanned in the wavelength ranging from 200-900 nm by using Perkin Elmer Spectrophotometer, and the characteristic peaks were detected. The FTIR spectrum confirmed the presence of alcohol, alkanes, amides, alkyl aldehydes, halogen, and aromatic compounds. The results of the GC-MS analysis provide different peaks determining...
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Posted by admin on Jan 31, 2019 in |
Congenital heart disease (CHD) is a defect in the heart or major blood vessels that are present in children at birth & CHD it occurs in approximately 1% of live births in developed countries. Infants and children with CHD exhibit a range of delays in weight gain and growth. Objective: To find out the relationship between the children with CHD nutritional status and their socio-demographic characteristics. Methodology: Non-probability (purposive) sample of (65) were selected of 225 children who visit the heart center during the period of conducting the pilot study, previously diagnosed with congenital heart disease. Results: The children with congenital heart disease have (32.3%) suffered from underweight by weight for age, a highly significant relationship between weight for age index and gender. (64.6%) of study sample suffered from wasting with weight for height, a significant high association between the premature baby ” is wasting” and children with CHD. (78.5%) Suffered from stunting with height for age. Almost half of the study sample suffered from malnourished (49.2%) by measuring...
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Posted by admin on Jan 31, 2019 in |
Japanese encephalitis is the most common arthropod-borne human encephalitis in the world. JEV clinical outcomes vary from complete recovery to recovery with neuropsychiatric sequelae to death. Contribution of T cells to the control and immunopathology of JEV infection has been reported in various studies. CD4+ T cells were found to be major T cells that has role in protection as well as affecting the clinical outcome in murine Japanese encephalitis model. Role of the CD4+ T cells subset in determining outcome of JEV infection is incompletely understood. Therefore, we sought to determine how quantitative difference in CD4+ T cell subsets influences JEV infection outcome. A total of 544 Acute Encephalitis Syndrome (AES) cases were admitted in ICU and AES ward of Kushinagar District Hospital during a period from Aug 2013 to Dec 2015. A diagnosis of JEV infection was based on clinical symptoms and anti-JEV IgM antibody in the acute phase cerebrospinal fluid (CSF) and serum samples measured by IgM antibody capture (MAC)-ELISA. Blood samples were drawn, and flow...
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Posted by admin on Jan 31, 2019 in |
Objective: To compare the routine biochemical and histological techniques with FTIR-ATR spectroscopic methods in diagnosing hyperlipidemia. Materials: Compositional changes in the serum and tissues reflects the healthy and pathological status of animals. FTIR-ATR was attempted as an additional tool for evaluating the composition in the serum as well as in tissues materials. To achieve this Wistar rats are administered with high cholesterol suspension with coconut oil orally for 30 days. Results: Hyperlipidemia achieved with oral feeding of cholesterol-rich diet (200 mg/dl) on rats with body weight of 180 ± 10.0 g led to a rapid progression of hyperlipidemia resulting might cause atherosclerosis. In blood, cholesterol level (269 ± 8.72), Triglyceride (194 ± 5.03), LDL (165 ± 5.10) increased and a decrease in the serum HDL level (39 ± 3.09) obtained in the cholesterol-rich fed hyperlipidemia was observed. FTIR- ATR spectral peaks were obtained (1165 cm-1 for ring vibration mode of C-O-H and C-O-C bonds cholesterol ester with phosphoric acid, 1742 cm-1 responsible for C=O group of cholesterol ester (HDL)...
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Posted by admin on Jan 31, 2019 in |
The rhizomes of Curcuma caesia Roxb. (Zingiberaceae) Are traditionally used in the treatment of various ailments and metabolic disorders like leucoderma, asthma, tumors, piles, bronchitis, etc. in Indian system of medicine. Considering the importance of natural products in modern phytomedicine, the antioxidant and ant mutagenic activities of C. caesia Roxb. rhizome extract and its fractions were evaluated. The antioxidant results indicate that the free radical-scavenging activity may be attributed to the high contents of phenolics and flavonoids with a higher reducing capacity. The IC50 values of in-vitro and ex-vivo antioxidant activities of Curcuma caesia was found for DPPH scavenging activity was 1.08 ± 0.06 μg/ml, nitric oxide scavenging activity was 37.50 ± 1.54 μg/ml, Hydroxyl radical scavenging activity was 10.08 ± 2.01 μg/ml, Superoxide Anion scavenging activity was 29.63 ± 2.07 μg/ml respectively. The in-vitro antimicrobial activity of different fractions obtained from the rhizome of Curcuma caesia was investigated against standard strain and clinical isolates of Staphylococcus aureus. The clinical isolates were found more sensitive for different fractions, than...
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