Posted by admin on Feb 28, 2019 in |
Free radicals induce numerous diseases by lipid peroxidation, oxidation of monosaccharides and DNA damage. In the present study, the marine plant seagrass Halophila beccarii was exploited to detect the phytochemical constituents and its antioxidant potential. The aqueous and organic extracts of Halophila beccarii portrayed the presence of various metabolites such as phenolic compounds, flavonoids, tannins, and saponins. The antioxidant properties of extracts of Halophila beccarii (Hydrocharitaceae) were studied in four in-vitro models viz. Total antioxidant activity, radical scavenging activity by DPPH reduction Assay, Scavenging of H2O2 and reducing power. The extract was found to contain large amounts of secondary metabolites such as phenolic compounds and flavonoids. Among all the extracts the methanolic extract showed significant antioxidant activity compared with standards. The experimental data provide ample evidence to utilize Halophila beccarii as a novel dietary agent for treating chronic metabolic diseases, such as diabetes and hyperlipidemia and could be used as a potential source for natural health...
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Posted by admin on Feb 28, 2019 in |
The aim of the present research work is to validate the apixaban content in bulk and pharmaceutical dosage formulation and validate it as per ICH guidelines. A simple, rapid, precise and highly selective spectrophotometric method was developed for estimation of apixaban in tablet dosage form by the area under curve method. The area under curve method involves the measurement of absorbances of apixaban at the wavelength of 269 nm-289 nm. Methanol was used as a solvent. Linearity was observed in the concentration range of 5-25 µg/ml for apixaban. The accuracy of the method was confirmed by recovery studies of tablet dosage forms and was found to be 100% for Apixaban. The method showed good reproducibility and recovery with % RSD less than 0.988%. The LOD of apixaban was found to be 0.335 µg/ml, and LOQ of apixaban was found to be 1.015 µg/ml. Thus the proposed method was found to be rapid, specific, precise, accurate and cost-effective quality control tool for the routine analysis of Apixaban in bulk and...
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Posted by admin on Feb 28, 2019 in |
The current study involves evaluation of antioxidant potential of selected nutraceuticals like barley grass juice (Hordeum vulgare L.), ethanolic extracts of cashew apple (Anacardium occidentale L.) and sea lettuce (Ulva reticulata F.) in in-vitro experimental model using goat liver slices at different concentrations (100, 200, 400, 800 and 1600 mg/kg, p.o). The oxidative stress was induced using hydrogen peroxide, and the levels of lipid peroxidation (LPO), superoxide dismutase (SOD), catalase, and reduced glutathione (GSH) were measured. Rutin was used as a standard. Barley grass juice, ethanolic extracts of cashew apple and sea lettuce have dose-dependently ameliorated the LPO, an indicator of membrane damage caused by oxidative stress. The selected nutraceuticals have significantly improved the levels of antioxidant enzymes like SOD, catalase, and GSH. Although the present study reported the antioxidant potential of the selected nutraceuticals, it still needs to be explored in different test systems employing different...
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Posted by admin on Feb 28, 2019 in |
Background: Chronic periodontitis (ChP) is the most common form of periodontal diseases. The progression rate may be influenced by local, systemic conditions and environmental factors that can alter the normal host response to bacterial plaque and affect the susceptibility to disease. Several studies have reported a role for vitamin D receptor (VDR) gene polymorphisms in numerous immunological activities. The present study purpose was to investigate whether FokI polymorphisms in the VDR gene were associated with the incidence of ChP in a Syrian population. Methods: The study sample comprised eighty Syrian subjects who were allocated into two groups. 50 patients were included in the group (ChP), whereas 30 matched individuals with no periodontal disease were included in the group (R). DNA was isolated from peripheral blood cells. Subsequently, genotyping of the (VDR) polymorphism was performed by a polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis using FokI enzyme. Statistical analysis was carried out to assess the differences between study groups. Results: Alleles T and C were present at...
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Posted by admin on Feb 28, 2019 in |
Diabetes is a group of metabolic disorders characterized by a chronic hyperglycemic condition resulting from defects in insulin secretion, insulin action or both. The chronic hyperglycemia in diabetes is associated with long term damage, dysfunction, failure of kidney, nerves and blood vessel. Nowadays, herbal treatments available for diabetics are eroding the share of allopathic formulations. Currently, the spray drying technique has been widely used for improving processability, biopharmaceutical performance as well as modifying the physical form of various phyto-extracts. Extract of mulberry being sticky may find difficulty in its formulation to tablet dosage form. Across the world, mulberry has been used for its medicinal importance. Looking at its availability and medicinal prospects spray dried mulberry extract was prepared and which is finally assembled into a tablet dosage form. Inert excipients are used to deal with processability, amorphous form and bioavailability issues. Spray drying of the mulberry extract was carried out by using carrier maltodextrin. Results obtained shows excellent/good flowability, compressibility, and compatibility. The dissolution profile of spray dried extract...
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