Posted by admin on Nov 30, 2018 in |
Objective: The purpose of the study was to evaluate the effect of an Ayurvedic formulation Varanadi kashayam to inhibit pancreatic lipase activity in-vitro. It also focuses on antioxidant activity, total phenol, flavonoid and phenolic acid content of fractions of Varanadi kashayam and HPTLC fingerprinting of fractions. Methods: Fractionation of Varanadi kashayam was done by using five solvents of increasing polarity. Qualitative analysis of phyto-chemicals and quantitative determination of phenol, flavonoids, and phenolic acids were determined by standard protocols. The antioxidant activity of fractions was measured by DPPH and ABTS scavenging assay through spectroscopic analysis. The anti-lipase activity of fractions of Varanadi kashayam was determined by using porcine pancreatic lipase as enzyme and p-Nitrophenyl palmitate as substrate. HPTLC analytical method was used for the determination of compounds presents in polyherbal formulation Varanadi kashayam. Results: Ethyl acetate fraction of Varanadi kashayam showed free radical scavenging activity with IC50 values close to that of BHT and quercetin standards. The amount of phenol, flavonoid, and phenolic acid was also found to be highest...
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Posted by admin on Nov 30, 2018 in |
The application of medicinal plants such as Terminalia arjuna to maintain health and treat disease started since time immemorial and still is a part of medical practice. The phytochemical analysis of bark of T. arjuna in methanolic, hydromethanolic (1:1), aqueous and petroleum ether extracts revealed the presence of carbohydrates, amino acid, alkaloids, saponins, tannins, flavonoids, terpenoids, glycosides, xanthoproteins and phenols. In-vitro antioxidant capacity by linear regression analysis was measured by assaying DPPH radical and H2O2 scavenging capacities. Their respective IC50 values were found to be 336.89 μg/ml and 253.01 μg/ml. The hydromethanolic extract gave total phenolic, total flavonoids and FRAP values of 754.16 ± 16.26 mg GAE/g, 247.02 ± 8.81 mg/g QE/g and 1.36 ± 0.03 mM FeSO4 respectively. The IC50 values for in-vitro anti-inflammatory activities were evaluated by the following assays: lipoxygenase inhibition (IC50 = 480.5 μg/ml), heat induced protein denaturation (IC50 = 282.32 μg/ml), proteinase inhibition (IC50 = 1339.3 μg/ml) and RBC membrane stabilization (IC50 = 192.81 μg/ml) at different concentrations using aspirin as control. Studies were...
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Posted by admin on Nov 30, 2018 in |
Waste disposal has become one of the major problems we are facing today. The rapid increase in the generation of huge extent of waste is one feature of the environmental crisis. This is accompanying with recent global development with respect to rapid urbanization and population growth which has resulted into development of large quantity of organic solid waste. Vermi composting has become progressively more popular across the word in recent decades; Vermicompost is the process which will convert waste into valuable fertilizer. The function of earthworms on soil ecosystem differs considerably with different earthworm species that are linked to their approach. In the present study was conducted to manage the different wastes obtained from erode district. The solid waste like Agriculture waste, Market waste, Industrial waste, Hospital waste and Household waste were collected and converted into vermicompost. The characteristics and nutrients elements of different vermicomposts are evaluated. Among all vermicomposts the agricultural waste, market waste and house hold waste gave potential...
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Posted by admin on Nov 30, 2018 in |
The aim of the present study is to find the effectiveness of two series of indenoisoquinolines compounds against cancer. Topoisomerase I (Top 1) an essential enzyme that participates in the processes associated with separation of DNA strands and manages the super helical tensions through the transient breakage of one strand of duplex DNA, monitored by the unwinding of supercoiled DNA. The Top 1-DNA complex an effective target in the treatment of cancer was taken as a receptor for study. The two series of indenoisoquinolines compounds were taken as ligand molecules and the 3D structure of human Top1-DNA was retrieved from PDB database. The structures of ligand were drawn using Chemsketch. Docking studies were performed using GOLD which predicts the binding affinity and hydrogen bond interactions between the ligand and receptor. From the results, it was observed that the selected series of both nitrated indenoisoquinolines and 2, 3 dimethoxy-substituted indenoisoquinolines had better Gold score with the active site residue ARG364. Docking studies conclude both the series may act as potent...
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Posted by admin on Nov 30, 2018 in |
The objective of the study was to investigate the effect of microemulsion on in–vitro skin permeation, percutaneous absorption of Irbesartan and to postulate probable mechanism of skin permeation. Microemulsion was prepared by aqueous phase titration method. Pseudoternary phase diagrams were constructed for the microemulsions. Thermodynamic stability study was carried out on selected microemulsions. Characterization of selected Irbesartan-loaded microemulsions was done by droplet size and polydispersity index determination. Physicochemical properties of Irbesartan-loaded microemulsions were determined using reported procedures. In-vitro skin permeation was determined using Franz cell. Skin permeation mechanism was assessed by Fourier Transform Infra – Red spectra analysis, Differential Scanning Calorimetry and activation energy measurement. A non-compartmental pharmacokinetic study was carried out to estimate the percutaneous absorption through the rat skin. The particle size and polydispersity index of the spherically shaped microemulsions were in the range of 83 – 86 nm and 0.270 – 0.290 respectively. In-vitro skin permeation study gave the permeability coefficient and steady state flux of Irbesartan to be 3.52 (×10-3) cm/h and 17.59 ± 0.38...
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