Posted by admin on Jun 30, 2018 in |
To develop new pulsatile release tablets of Rabeprazole sodium (RS), that can suppress drug release in stomach and release the drug rapidly after a predetermined lag time for about 4 h in intestine, a multiple unit dosage forms was designed that contain, drug-containing core, a coating to achieve time lag in drug release and an outermost acid resistant enteric coating. Time lagged press coating was performed using ethyl cellulose (EC) and different grades of hydroxypropyl methylcellulose (HPMC) in different ratios. The press-coated tablets were optimized by the drug release study and finally the formulation F17 containing 7:1 ratio of EC and HPMC K4M was selected as optimized formulation. This press coated tablet was further enteric coated with Cellulose acetate phthalate (CAP). The weight gain of the enteric coating was optimized based on the integrity of coating in an acidic solution for about 2 h. Formulation F21 was finally selected with 10% weight gain and rupture time of 120 minutes in 0.1N HCl. Further, the drug release from F21 formulation...
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Posted by admin on Jun 30, 2018 in |
This study evaluated the acetone extract of Crinum jagus bulbs for its antioxidant, and antiplasmodial properties. The bulbs of Crinum jagus are used in traditional medicine for various ailments. The antioxidant activity of the extract was evaluated using DPPH and nitric oxide free radical scavenging assay. The antiplasmodial activity (suppressive, prophylactic and curative activity) was evaluated in-vivo against Plasmodium berghei at 250 mg/kg and 500 mg/kg through the oral route in Swiss albino mice. Isolation and characterization of the active principles were done using GC-MS techniques. Phytochemical analysis of the extract revealed the presence of flavonoids, tannins, saponins, steroids and alkaloids. The LD50 was found to be greater than 5000 mg/kg. The extract showed a dose-dependent antioxidant activity against the various assays. The extract caused dose-dependent and statistically significant (P < 0.05) reduction of parasiteamia in the antiplasmodial suppressive and prophylactic study. Bowdensine and demethoxy bowdensine were the active principles found on chemical analysis of the extract, using GC-MS. The results indicate that this extract may possess antioxidant, prophylactic...
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Posted by admin on Jun 30, 2018 in |
Tridentate Schiff bases were obtained in succession from the condensation of ethyl 2-amino-5,6-dihydro-4H-cyclopenta [b] thiophene-3-carboxylate and different aryl aldehydes in 1:1 molar ratio. Zinc chloride complexes of these new aminothiophene Schiff bases were prepared in 1:1 metal: ligand stichometry. These complexes were characterized using IR, UV-Visible, elemental analysis and molar conductivity. Schiff bases displayed monobasic tridentate coordination with central Zn (II) ion and formed tetrahedral complexes. These complexes were evaluated for their antimicrobial activity against gram-negative, multidrug-resistant Metallo- β-lactamase (MBL) and Extended Spectrum β-lactamase (ESBL) producing uropathogens and for their antitubercular activity against Mycobacterium tuberculosis (H37Rv strain) to observe improved biological activity compared to parent Schiff...
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Posted by admin on Jun 30, 2018 in |
Pyrazoline derivatives, being used as potential medicinal agents, 3-Acetyl-2H-chromen-2one (I) was prepared by Knoevenagel condensation of salicyladehyde with ethylacetoacetate in presence of piperidine. A series of 3-[(2E)-3-substituted-prop-2-enoyl]-2H-chro men-2-one derivatives (II a-h) were prepared by Claisen-Schmidt condensation of 3-acetyl coumarin with aromatic aldehydes. Treatment of 3-substituted cinnamoylcoumarin with hydrazine hydrate in the presence of ethanol gave [5-substitutedphenyl]-4, 5-dihydro-1H-pyrazol-3-yl]-2H-chromen-2-one (III a-h). Title compound were synthesized by conventional as well as by microwave assisted method. The structures of the newly synthesized compounds were confirmed by IR and 1H-NMR spectroscopy. All the synthesized compounds were tested for their antibacterial and antifungal activities using cup-plate-agar-diffusion method. The antibacterial activity screening revels that the compound III b has comparable activity and compound III c shows moderate activity as that of standard ampicillin against gram positive and gram negative bacteria. All synthesized compounds were found to be inactive as antifungal against Candida...
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Posted by admin on Jun 30, 2018 in |
The present study aimed to develop and validate stability indicating method for the simultaneous determination of Elbasvir and Grazoprevir in its pharmaceutical dosage form using RP-HPLC. Chromatographic separation was done with Discovery C18 (250 mm × 4.6 mm, 5 µ) column using 0.1% ortho-phosphoric acid (OPA) and acetonitrile in the ratio 50:50% v/v as mobile phase on isocratic mode. The column oven temperature was maintained at 30 ºC with a flow rate 1.0 ml/min and components were detected at a wavelength of 315 nm. The retention times for Elbasvir and Grazoprevir was found to be 2.24 min and 3.21 min respectively. The developed method was validated according to ICH guidelines. A good linearity response was observed in the concentration range of 12.5 µg/ml – 75 µg/ml for Elbasvir and 25 µg/ml – 150 µg/ml for Grazoprevir with correlation coefficient of 0.999 for both the drugs. The method was found to be accurate, precise, specific, rugged and robust. The drugs were subjected to stress conditions for testing their stability and...
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