Posted by admin on Mar 31, 2018 in |
This study aimed to characterize and evaluate chitosan nanoparticles (CSNPs) as a carrier system for the hormone, oxytocin. Ionotropic gelation was the technique used to synthesize the CSNPs. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed narrow particle size distribution of 30-50 nm and spherical particle shape. Differential scanning calorimetry (DSC), X-ray powder diffraction (XRD), thermal gravimetric / differential thermal analysis (TGA / DTA) and Fourier Transform Infrared Spectroscopy (FTIR) were used to evaluate possible drug-polymer interactions. Data obtained from X-ray diffraction (XRD) showed a decrease in crystallinity due to the disruption of the intramolecular / intermolecular chitosan network and a molecular level of oxytocin dispersion in the O-CSNP matrix. Differential scanning calorimetry (DSC) exhibited further evidence of drug-polymer interaction through observed endothermic shifts. Fourier-transform-infrared (FT-IR) spectra obtained confirmed the presence of oxytocin within the CSNP matrix as well as further proof of the intermolecular interactions existing between oxytocin and chitosan. Loading and release profiles of the O-CSNPs were conducted using LC/MS. The effect of nanoparticle size...
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Posted by admin on Mar 31, 2018 in |
Introduction: Klebsiella pneumoniae is one of the most frequent infection organism following orthopedic surgeries and specifically total hip arthroplasty. Recently, attitude toward herbal medicine is being popular both in people and health care systems. Ruta graveolens is a traditional herbal remedy Iranian medicine and some other countries with a wide spectrum of therapeutic utilities. Our purpose was to determine the effects of this medicinal plant on Klebsiella pneumoniae growth. Materials and methods: Standard Klebsiella pneumoniae was used. We assessed the hexane extracts of both leaf and stark of Ruta graveolens on growth of bacterial disc diffusion and serial dilution methods. We compared the results with some standard frequent chemical antibiotics. Results: Our results showed that all doses of leave extract including 200, 250, 300, 350 and 400 micrograms of stark extract had inhibited the growth. In disc diffusion test 500 μg of hexanic extract prevent the growth of bacteria rather than antibiotic discs. We determined minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) by doing their methods. Conclusion:...
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Posted by admin on Mar 31, 2018 in |
Objective: The flavonoid baicalein, is historically used as an herbal medicine by the Chinese, which produces a wide range of pharmaceutical effects, among which is the potent anticancer activity which has raised a great conflict of interest in the recent years. This study aims to verify the anti-proliferative and the cell viability effects of baicalein on metastasis of MCF-7 human breast cancer cell lines by in-vitro, to investigate the related mechanisms. Methods: MTT assay was used to investigate the anti-proliferative activity of baicalein using MCF-7 cell lines and trypan blue assay method were used to examine the effects of baicalein on cell viability on MCF-7 cell lines. Results: The in-vitro cytotoxicity activity studies were proved that cancer cell lines were inhibited significantly with the increasing of sample...
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Posted by admin on Mar 31, 2018 in |
Ethanolic extract of the aerial parts of Thymus serpyllum was evaluated for antioxidant activity. As many diseases in today’s world are due to free radicals, finding antioxidants of herbal origin can lead to development of safe herbal medicines for these diseases. Hydroxyl radical scavenging method was used to evaluate the antioxidant activity of Thymus serpyllum in the present study. The results obtained from the study proved the hydroxyl radical scavenging activity of Thymus serpyllum. Ethanolic extract (99.9%) of aerial parts of Thymus serpyllum was prepared, dried and sent to Deshpande Laboratories Private Limited, Madhya Pradesh Bhopal (An ISO 9001:2008 Certified Drug Testing Laboratory) for analyzing hydroxyl radical scavenging activity. The results obtained from the study proved the free radical scavenging of the extract. The antioxidant effect of Thymus serpyllum can be largely attributed to its chemical constitution. Since Thymus serpyllum showed significant antioxidant activity, it can be developed into a safe herbal formulation useful in stress and stress related...
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Posted by admin on Mar 31, 2018 in |
The purpose of this study was to design and evaluate Amoxicillin trihydrate floating granules by melt granulation technique using Gelucire 43/01 and Campritol 888 ATO as lipid carriers. Polymers HPMC K4M and Ethyl Cellulose as release rate modifiers. The granules were evaluated by FTIR studies, differential scanning calorimetry, Scanning Electron microscopy, drug content, in vitro floating ability and in vitro drug release. The optimized formulation showed good floating ability and 98% drug was released in 12 h with both lipid carriers. The drug release from optimized formulations F3 and F9 followed zero order patterns and governed by Non-Fickian mechanism which was confirmed by the ‘n’ values of Koresmeyer Peppas equations. Formulations of Gelucire 43/01 had shown superior retardation and floating characteristics compared to that of Campritol 888 ATO. In conclusion, hydrophobic lipid, Gelucire 43/01 can be considered as an effective carrier for design of a multiunit floating drug delivery system of highly water – soluble drugs such as Amoxicillin...
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