Posted by admin on Apr 30, 2018 in |
ABSTRACT: To find new antiviral agents from natural sources, methanol and aqueous extracts of leaves and fruits of Ribes uva –crispa and Ribes multiflorum naturally grown in Turkey were investigated as in vitro to reveal their antiviral activities against Herpes simplex virus type 1. All experiments were performed in 96-well plates and results were measured with XTT-based colorimetric assay. Results demonstrated that all extracts have no cytotoxic effect on vero cells at 10000 µg/mL, while they also exhibited anti-HSV-1 activity with different percentages of protection (varying between 2.65% – 50.40%) in 10000 µg/mL which was at the highest concentration in vero cells. 50% effective concentrations (EC50) of the extracts which were determined having percentage of protection against HSV-1 at concentrations lower than 10000 µg/mL were calculated using GraphPad Prism Version 5.03 statistics program with non-linear regression analysis. These extracts were determined to have EC50 values ranging between 9710 – 70600 µg/mL and selectivity index (SI) are ranging between 0.14 – 1.03. On the basis of these results we believe...
Read More
Posted by admin on Apr 30, 2018 in |
Objective: Pergularia daemia and Terminalia catappa L.. Leaf extracted with hydroethanol and the extracts were separated by silica gel column chromatography. The selected column fractions are used in GC-MS analysis, from the GC-MS results eight phytochemical compounds are selected based on their biological activities. In the present work we predict the interaction of the selected targeted proteins (CYP2E1, UDP-GT, GST and Nrf2 binding site in keap1 protein,IL-6) with bioactive compounds of the extracts (HAEPD and HAETC) by molecular docking studies. The number of bioactive compounds present in HAEPD and HAETC, and there phytochemicals are responsible for various biological as well as hepatoprotective and anti hepatotoxic properties of the plant...
Read More
Posted by admin on Apr 30, 2018 in |
Introduction: Based on the results of formerly conducted studies, the application of invasive methods, such as blood transfusion, for the treatment of children with thalassemia cause distress and anxiety symptoms in these children; therefore, it is attempted to apply non-invasive, non-pharmacological, simple, and inexpensive nursing practice to improve these deficiencies. Thus, the present study was conducted to investigate the effect of foot reflexology massage on anxiety caused by blood transfusion in children with Thalassemia. Methodology: The present quasi-experimental study was carried out on 60 children with thalassemia who qualified according to inclusion criteria. Patients were randomly divided into three groups of intervention (recipient of reflexology massage), placebo (common massage), and control. The anxiety level of the patients with the anxiety scale was evaluated based on the obsessive-compulsive disorder (osbd-r) 20 minutes before transfusion. Patients of intervention group received 10 minutes of reflexology massage and those categorized in placebo group received 10 minutes of common massage. After transfusion, the anxiety level of the patients was measured; collected data was analyzed...
Read More
Posted by admin on Apr 30, 2018 in |
The present study was carried out to evaluate antidiabetic as well as in-vivo and in-vitro antioxidant activities of n-hexane fraction of Tamarindus indica Linn. seed (T. indica) in streptozotocin (STZ) induced diabetic rat. Oral administration of n-hexane fraction at the dose of 100 mg/kg body weight for 28 days prevented significantly the STZ-induced hyperglycemia. The plasma insulin and C-peptide levels as well as activities of antioxidant enzymes such as catalase (CAT), peroxidase (Px) and superoxide dismutase (SOD) in the hepatic tissue were found to be decreased in diabetic animals which were corrected after the treatment of n-hexane fraction of hydro-methanolic extract of T. indica. Oral glucose tolerance test (OGTT) reveals that the fraction at above mentioned dose showed a significant decrease of blood glucose level in normal and diabetic rat. Histopathology of pancreas was performed after n-hexane fraction treatment to diabetic rat and the results were compared with the control as well as diabetic groups. To evaluate the free radical scavenging activities of the n-hexane fraction following in-vitro study...
Read More
Posted by admin on Apr 30, 2018 in |
In this present research work, the aim was to develop ileo-colonic targeted mini-tablets filled capsule system of flurbiprofen for treatment of rheumatoid arthritis. In the present study pH independent colon targeted system was developed and evaluated. SMEDDs of poorly water soluble drug flurbiprofen were prepared to enhance its dissolution rate and bioavailability. Mini-tablets of flurbiprofen were also prepared and filled in capsule. This capsule was coated with ethyl cellulose. Based on in-vitro dissolution studies it was noted that ethyl cellulose release takes place in colon (colon targeted). The design of formulation hence ensured colon targeted delivery of drug preventing premature drug release in stomach or small intestine and enhanced dissolution rate for enhancement of bioavailability. In vitro release studies were carried out in 0.1 N HCl pH 1.2, phosphate buffer pH 7.4 and phosphate buffer pH 6.8 mimicking in-vivo conditions for dissolution. SMEDDS exhibited a considerable increase in solubility of flurbiprofen and selected colon targeted formulation of pure drug successfully achieved colonic release of flurbiprofen with a lag time...
Read More
