Posted by admin on Nov 30, 2017 in |
Background: Antimicrobial resistance against S.aureus infections is a global public health problem resulting in very limited treatment options, and inappropriate use of antibiotics is one of the most important factors that could affect the increasing pattern of resistance. Objective: To determine the antimicrobial resistance pattern of S. aureus among healthy and adult students to commonly used antimicrobial agents. Materials and Methods: This study was carried out at Cytogene Research and Development laboratory, Indira Nagar, Lucknow, India. Samples from throat were collected as throat-swab which were cultured and screened for S. aureus using standard microbiological protocols. The antimicrobial susceptibility of identified S. aureus was evaluated using disc diffusion technique. Results: A total of 47(54%) S. aureus were identified from 87growths of the 185throat swab samples. All the S. aureus isolates were methicillin resistant; they exhibited total resistance to penicillin, 100% to Erythromycin, 97.8%to Amoxicillin, 91.4% to Clindamycin, 78.7% to Vancomycin, 53.1% to Gentamicin, 44.6% to Trimethoprime, 19.1% to Tetracycline and 6.3% to Ciprofloxacin. Conclusion: The resistance to antibiotics agents is...
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Posted by admin on Nov 30, 2017 in |
The present work deals with formulation of topical gels containing aqueous extract of P. acerifolium seeds and also to evaluate the prepared gels against some Gram-positive and Gram-negative bacteria. The antibacterial activity of various P. acerifolium seed extracts was measured through determining minimum inhibitory concentration (MIC) by cube dilution method and zone of inhibition by disc diffusion method against E. coli, P. aeruginosa and S. aureus. Aqueous P. acerifolium seed extracts exhibited comparatively higher antibacterial potential in comparison with that of other extracts and employed to formulate topical herbal gels using 4.5 % sodium carboxymethyl cellulose as gel base. Prepared herbal gels were tested for pH, viscosity, extrudability and spreadability. All the prepared gels were subjected to antibacterial study by determining zone of inhibition by disc diffusion method. Herbal gels containing 15 % aqueous P. acerifolium seed extract exhibited better zones of inhibition against E. coli, P. aeruginosa and S. aureus in comparison with that of 5 % and 10 %. From the results of this study, it can...
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Posted by admin on Nov 30, 2017 in |
The objectives of the present study were to evaluate the preliminary phytochemical analysis (quantitative and qualitative) and DPPH antioxidant activity of two traditionally important plants occurring at Purulia district of West Bengal in India. Qualitative analysis of phytochemicals (flavonoid, alkaloid, terpenoids, saponins, phenol and carbohydrate) and quantitative analysis of total phenolics and flavonoids were done by using suitable test protocols available in the literature. Antioxidant activity was studied through DPPH assay. Qualitative phytotochemical analysis revealed that the methanolic extract exhibit richness in flavonoids, alkaloids, terpenoids, saponins, phenols and carbohydrates in comparison with aqueous extract. Total phenol and flavonoid content shows highest concentration in methanolic part of A. aspera (1.129mg GA EQ/gm, 3.22mg QEQ/gm) and lowest concentration in aqueous part of S cordifolia (.348mg GA EQ/gm, .324mg QEQ/gm). EC50 values of the two plants were 48.06µg/ml in A. aspera and 52.43µg/ml in S. cordifolia. The results conclude that there is positive correlation between total phenol content and DPPH antioxidant activity. The plants could serve as potential source of natural...
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Posted by admin on Nov 30, 2017 in |
Plants possess good antioxidant capacity to get rid of free radicals that cause many lifestyle diseases especially cancer. Assessment of Antioxidant activity of Amaranthus Linn. leaves were evaluated by using in- vitro model. In the present study evaluation of ethyl acetate of extract of Amaranthus Linn. leaves with the help of two In-vitro antioxidant models were carried out for Nitric-oxide scavenging Activity and DPPH method. IC50 value was calculated and compared with standard Ascorbic acid. Ethyl acetate extract was found to be extremely effective in scavenging nitric-oxide (IC50 584.4 μg/ml to 1072 μg/ml). In inhibition of DPPH Radical Scavenging activity (IC50 260.6 μg/ml to 957 μg/ml). Ethyl acetate extract showed different levels of antioxidant activities in tested...
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Posted by admin on Nov 30, 2017 in |
The alcoholic extract of fruits of the plant Luffa acutangula previously reported for is hepatoprotective activity was fractionated into three parts to chemically identify the most potent bioactive fraction. The hepatoprotective potential of the fraction prepared from extract was studied in vivo in rats as well as ex vivo in isolated hepatocytes against paracetamol induced hepatotoxicity. The hepatoprotective activity was determined on the basis of their effects on parameters like direct bilirubin, serum aspartate transaminase, serum alanine transaminase and alakaline phasphatase. Ethyl acetate fraction (EAF) of alcoholic extract 100 mg/kg, p.o.) exhibited maximum hepatoprotective activity and is the active fraction for hepatoprotective activity of L. acutangula...
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