Posted by admin on Jul 31, 2017 in |
Protein conformation and dynamics are influenced by various factors, including binding of ligands, their physico-chemical properties, etc. Every conformational change is dictated by an array of events, as making and breaking of bonds or change in interaction of protein residues. The three dimensional structure of protein molecules are widely investigated, based on small world network approaches, with an emphasis on different combinations of descriptors affecting the structure which have been tested on studies involving binding in protein ligand complexes and for protein-protein complexes. This application has revealed the benefits and success of the small world network approach which can change the focus from specific interactions in the local environment or to non-local phenomenon. Network analysis of interacting protein upon ligand binding is analysed. A similarity in interaction parameters among residues of the target protein, upon binding of particular ligands, is identified. This method differentiates ligands, on the basis of overall changes in interaction among residues of Target proteins in...
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Posted by admin on Jul 31, 2017 in |
Microalgae have been widely used as novel source of bioactive substances. These substances exhibit various biological actions including, antioxidant, antimicrobial, antiviral, antitumoral, anti-inflammatory and anti-allergy effects. In the present investigation algal biomass in methanol extracts of chlorophyceae member of three micro algae of Chlorella vulgaris, Desmococcus olivaceous and Chlorococcum humicola was screened. The antioxidant property of the methanolic extracts of these green microalgae was evaluated by measuring the free radical scavenging activity by DPPH assay method. The algal extracts were then evaluated for their suppressive effect on tumor cell growth (MCF7) by using MTT assay and further analysed using GC-MS to determine the profile of specific molecules or compounds. Antioxidant activities of the microalgal methanol extracts were studied by their ability to scavenge DPPH at various concentrations. The DPPH radical scavenging activity was found to be higher in Chlorella vulgaris (RSA 53.96%) at a concentration 20μg compared to standard ascorbic acid (RSA 59.42%). Anticancer property of the methanol extracts of Chlorella vulgaris, Desmococcus olivaceous and Chlorococcum humicola in MCF7...
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Posted by admin on Jul 31, 2017 in |
Present paper reports the chemical constituents in the fatty acids obtained from the whole plant of diploid and tetraploid Physalis angulata Linn. collected from hot desert areas of Rajasthan. The fatty acid analysis was carried out by gas chromatography-flame ionization detector (GC-FID). Meiotic studies confirm the ploidy level of the plant. Diploid (n = 12) cytotype is reported from Udaipur district and tetraploid (n = 24) cytotype from Sri Ganganagar. There is a considerable difference in the fatty acid constituents in the cytotypes. Major constituents of fatty acids are Oleic acid (24.93%, 2x; 18.71%, 4x); capric acid (13.4%, 2x; 0.14%, 4x); Palmitic acid (6.966%, 2x; 12.38%, 4x); Linoleic acid (5.5%, 2x; 9.76%, 4x) and Linolenic acid (6%, 2x; 11.67%, 4x). The main objective of the object work was to compare the fatty acid composition within two cytotypes. Such analysis of plants is very important on commercial level for the productions of cosmetic and has great interest in pharmaceutical companies for the production of...
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Posted by admin on Jul 31, 2017 in |
Matricaria pubscens (Asteraceae), with wide distribution area in southern Algeria, is a drug traditionally used in the treatment of certain diseases such as dehydration, cough and sore. The aim of this study was to determine the anatomical features of the root stem and leaves of medicinal species of M. pubscens sections. As a result of the study, it was discovered that in the root the vessels arranged in short multiple radial; the fibers are thin-walled thick, Vascular bundles are separated by large unlignifid rays.Most vessels in the central part are plugged with dark-staining substances. The anatomical structure of the leaves is isobilateral mesophyll and palisade parenchyma nearly two epidermises and spongy parenchyma in the middle, the leaf was bifaceous. The surface of the epidermis is covered with glandular hairs, the stomata were diastatic. The extract of aerial part of M. pubscens has significant amounts of Phenols, Flavonoids and Steroids, however tannins are present an average amount; the Alcaloids are present in lowest...
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Posted by admin on Jul 31, 2017 in |
The present study is based on the comparative evaluation of preliminary phytochemical screening and in vitro antimicrobial activity of methanolic and ethanolic extracts of Stevia rebaudiana leaves. The crude extracts of S. rebaudiana leaves were subjected to phytochemical screening tests by using standard procedures for the detection of the presence or absence of eight major secondary metabolites. The free radical scavenging activity of both the extracts was measured by using the 1,1- diphenyl-2-picrylhydrazyl (DPPH) assay. The dose dependent increase was observed in DPPH activity of methanolic and ethanolic extracts (10, 20, 40, 50, 100 µg/ml), and the range was 28.65 – 64.35 % and 28.15 – 61.57 % as compared to ascorbic acid 30.15 – 85.99 %. The antibacterial activity of S. rebaudiana leaves was tested by agar-well diffusion assay. The plant extracts of four different concentrations (25, 50, 75 and 100 mg/mL) were prepared in DMSO and tested against eight bacterial strains and was compared with that of standard drug tetracycline (10 µg/mL). The results of preliminary phytochemical...
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