Posted by admin on Mar 31, 2017 in |
Suddhi is a unique process of detoxification which is employed topotentiate as well as enhance therapeutic properties the effect of raw drugs. Nabhi (Aconitum ferox), a herbal poisonous rhizome commonly known as aconitum is used extensively in various Siddha formulations, with great therapeutic significance. Siddha system recommends the administration of Nabhi only after suddhi (purification) in different medias. Hence in this pretext one of the purification process of Nabhi mentioned in Theraiyar Yemaga Venba book gets validated scientifically with alleged reasoning through estimation of aconitine by HPTLC method and along with physicochemical parameters.The amount of Aconitine present is found to be reduced from 0.2107 % to 0.00 % after purification. In this present scenario may be presumed that the toxic compound aconitine might have been underwent transformation into some nontoxic compounds like Benzyl aconine and...
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Posted by admin on Mar 31, 2017 in |
Diacerein is an Osteoarthritic drug that exhibited suboptimal oral bioavailability upon oral administration of conventional dosage form. Nanosuspension gaining more attention for improving oral bioavailability of such drugs. This study was aimed to enhance the solubility and dissolution of diacerein by preparing Nanosuspension. Diacerein Nanosuspension was prepared using combination of High Speed Homogenization (HSH) and Media Milling (MM), Poloxamer 407 (stabilizer) and ZrO2 beads (milling media). Various formulation and processing parameters were optimized in preliminary studies. Concentration of stabilizer and milling media were optimized for Cumulative percentage release (%CPR), saturation solubility (SS) and mean particle size (MPS) using 32full factorial design. Optimized batch was derived statistically using desirability function of Minitab17. Model was validated by formulating checkpoint batch. Accelerated stability study was carried out for optimized batch. Identification and compatibility study of drug and stabilizers were carried out using FTIR and DSC studies. Preliminary parameters were optimized by varying one parameter at a time, while keeping other constant by trial and error method. Optimized batch has 100%w/v milling media...
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Posted by admin on Mar 31, 2017 in |
Aims: To evaluate and analyze effectiveness of animal simulation based teaching in pharmacology in comparison to conventional methods to undergraduate medical students. Methods: An interventional and comparative study was conducted among second professional MBBS students. All students were divided into two groups A and B. Four demonstrations of experimental pharmacology were demonstrated by conventional method and animal simulation method by same teacher. Both groups were equally exposed to both modes of demonstrations. Every demonstration was followed by an assessment containing thirty objective type questions. Feedback was taken to analyze generalized opinion about the programme on likert’s scale. The data was analyzed by using Fisher’s exact test and p value calculated. Results: Percentage of students who scored 60% or more was higher in simulator based teaching in assessment related to procedure, related pharmacology and applied pharmacology. The difference between total scores of both groups in all four demonstrations was statistically significant with p value < 0.05. In feedback, percentage of students who opted three or more on likert scale was...
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Posted by admin on Mar 31, 2017 in |
The present study focuses on the ‘antioxidant approach’ as a therapeutic intervention for Parkinson’s disease (PD). It deals with understanding of the effects of Ocimum sanctum extract on oxidative and nitrosative stress biomarkers, responsible for neurodegeneration. The standardized hydromethanolic extract of O.sanctum, Linn (Os HM) at the dose of 50, 100 and 200 mg/kg were evaluated for their neuroprotective effects against MPTP induced Parkinsonism. Single divided doses of MPTP were used for induction of parkinsonism in male mice. L-DOPA rich and free fractions of Mucuna pruriens and Bromocriptine were considered as positive standards. The antioxidant effect and thus the neuroprotective potential was estimated based upon the levels of protein oxidation products, endogenous enzymatic and non-enzymatic antioxidant pool of glutathione, nitric oxide, superoxide dismutase and catalase. The results revealed that Os HM extract, particularly at the doses of 100 and 200 mg/kg, significantly depleted the protein carbonyl content, restored the decreased levels of GSH. Os HM prevented the observed compensatory response with superoxide dismutase levels against MPTP treatment. It also...
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Posted by admin on Mar 31, 2017 in |
Colocasia esculenta corm aqueous extract was assessed for its in vitro antioxidant capacity and free radical scavenging potential by seven different assays, viz. total phenolic content, total flavonoid content, total flavonol content, reducing power estimation as well as 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide (NO) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging activities. Results reveal that the total phenolic, total flavonoid and total flavonol content present in C. esculenta corm extract were found to be 68.25 ± 17.52 mg of GAE (Gallic Acid Equivalent)/g, 26.70 ± 40.18 mg of QE (Quercetin Equivalent)/g and 1.40 ± 53.99 mg of QE/g of dry extract respectively. Reducing power of the extract was found to be concentration dependent as it increase with increase in concentration and it was maximum at the highest evaluated concentration of 200 µg/ml. Significant antioxidant efficacy of C. esculenta corm was further confirmed by IC50 values of DPPH, NO and ABTS radical scavenging assays as they were found to be 74.34 ± 12.17 µg/ml, 65.52 ± 7.76 µg/ml and 132.29 ± 14.13...
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