Posted by admin on Jan 31, 2017 in |
The extracts of Spinacia oleracia leaves studied for Phytochemical screening and different extract of Spinacia oleracea leaves was studied for immunomodulatory activity. The immunomodulatory effect was evaluated by delayed type hypersensitivity response using SRBCs. The n-hexane, dichloromethane, methanolic and aqueous extract of Spinacia oleracea leaves was studies for DTH response. The dichloromethane extract of Spinacia oleracea leaves extract showed significant increase in the hypersensitivity response, indicating its effect on cell mediated immunity. From the above result, it was concluded that the preliminary Phytochemical analysis revealed that different active constituent present in different extracts such as carbohydrates, proteins, amino acids, fat, oils, steroids, terpenoids, glycosides, alkaloids, tannins and other phenolics compounds and the dichloromethane extract of Spinacia oleracea has a significant effect on cell mediated...
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Posted by admin on Jan 31, 2017 in |
An HPLC method was developed with the aim of quantifying the levels of Methyl Vinyl Ketone (MVK), a Potential Genotoxic Impurity (PGI), in Ivabradine HCl. A limit level concentration of 0.5 ppm for MVK was calculated by applying the concept of Threshold of Toxicological Concern (TTC). The developed HPLC method employs a Phenyl analytical column (150 mm х 4.6 mm, 3.5 μ), 5-μL injection volume, mobile phase of buffer (pH 2.5)-acetonitrile [gradient elution], a column temperature of 50°C and a flow rate of 1.0 mL/min. The retention time of MVK obtained was approximately 4.0 minutes in a program with run time of 22 minutes. The Limit of Detection was 0.06 ppm and Limit of Quantitation was 0.2 ppm. The method proved to be sensitive, linear, accurate and precise in the specified range (LOQ to 150 % of limit level concentration). The validated method was applied for identification and quantification of MVK in different batches of the...
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Posted by admin on Jan 31, 2017 in |
The study involved both aqueous extraction and methanolic extraction of the powered leaves, which ensure all plant components are extracted for better results. Phytochemical analysis from the extracts was performed using the standard protocols. The antimicrobial susceptibility of the extracts was tested against E-coli, Pseudomonas and Klebsilla sp using agar disc diffusion method. Antioxidant property of the plant was evaluated using DPPH Method and Hydrogen Peroxide Scavenging activity method whereas antidiabetic property was evaluated using α-amylase inhibition assay. The plant extracts were used at varying concentrations to ensure which plant extract and concentration causes the most inhibition. Phytochemical analysis of the extract indicated the present of Saponin, phenol , phytosterol, steroid, terpenoid, flavanoid, carbohydrates and proteins while tannin, glycoside, plobatanin were absent. Anti-diabetic property of the plant was observed with a significant result of inhibiting a maximum of 83% of α-amylase activity (700µg/ml concentration of methanol extract) whereas antioxidant property with a maximum of 85% of (200µg/ml concentration of water extract) inhibition. Antimicrobial property of the extract shows effectiveness...
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Posted by admin on Jan 31, 2017 in |
The aim of the present study was to prepare and evaluate floating microspheres consisting of (i) calcium silicate (CS) as porous carrier; (ii) famotidine and (iii) hydroxypropyl methylcellulose (HPMC) and ethylcellulose (EC) as polymers. The floating microspheres were evaluated for particle size, micromeritic properties, percent drug content, in vitro floating behavior, and in vitro drug release. The % yield of formulations (FM1 to FM9) was found to be in the range of 79.51 ± 3.71 to 93.48 ± 0.94 %. Percentage drug content of floating microspheres formulations (FM1 to FM9) was found in the range of 77.25 ± 0.36 to 86.14 ± 2.04 %. In Vitro Buoyancy percentage of the microspheres was found to be 97.5 ± 1.53 %. At pH 1.2, the best formulation FM4 showed maximum drug release (99.26 ± 1.14 %) at the end of 12 hr. The SEM photographs of formulation FM4 showed that the fabricated microspheres were spherical with a smooth surface and exhibited a range of sizes within each batch. The in vivo...
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Posted by admin on Jan 31, 2017 in |
The aim of this study was to analyze the chemical composition of dried clove buds volatile oil in Bangladesh by Gas Chromatography-Mass Spectrometry (GC-MS) technique and also show their medicinal properties according to the characteristics of the constituents present in that essential oil. In this experiment the clove buds essential oil was extracted by hydro distillation method. The major constituents obtained from the oil includes 3-Allyl-6-methoxyphenol i.e. m-Eugenol (69.44%), Eugenol acetate (10.79%), 4-hydroxy-4-mehtylpentan-2-one i.e. Tyranton (7.78%) Caryophyllene (6.80%), 1,4,7-Cycloundecatriene, 1,5,9,9-tetramethyl-,Z,Z,Z-and trace amounts (<1%) of other constituents. Eugenol is present in concentration of 69.43% which is the major volatile constituent of clove bud essential oil. It has wide range of medicinal value such as antiseptic and anaesthetic analgesic, antioxidant, anti-inflamatory and antimicrobial activities. Clove oil is used in dental care and it can relieve toothache temporarily. So, from these chemical constituents it is clear that clove buds essential oil of Bangladesh has medicinal properties because of the medicinal characteristics of these...
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