Posted by admin on Sep 30, 2016 in |
A novel reverse phase liquid chromatographic method has been developed for the simultaneous estimation of Atorvastatin Calcium, Ezetimibe and Fenofibrate in bulk and Pharmaceutical formulations by using reverse phase Agilent 100-5 C18 column [250mm x 4.6mm]. The mobile phase (Methanol: water) in the ratio of 70:30% v/v was pumped at a flow rate of 1ml/min and the column effluents were monitored at 250nm using Variable Wavelength UV detector. Linearity was obtained in the concentration range of 20-100 µg/ml for Atorvastatin and Ezetimibe and 50-250µg/ml for Fenofibrate. The established method was statistically validated according to the ICH Q2B guidelines and the percentage relative standard deviation for precision, robustness and ruggedness was found to be less than 2% indicating high degree of precision and robustness. The percentage recovery for the accuracy was found to be 100.52%, 100.45% and 101.31% for Atorvastatin, Ezetimibe and Fenofibrate respectively which were within the specified limits of recovery. Assay for the marketed formulation proved that 99.86% of Atorvastatin, 99.61% of Ezetimibe and 99.83% of Fenofibrate. Hence...
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Posted by admin on Sep 30, 2016 in |
The main aim of the present study is to formulate low cost natural mosquito paper coil and to evaluate the mosquitocidal activity of Annona squamosa leaf extracts. Annona squamosa Linn. is the plant belonging to the family of Annonaceae commonly known as custard apple. Several bioactive compounds have been isolated from the Annona squamosa leaf extracts reported to possess anti-diabetic activity, Mosquitocidal activity, antioxidant and anti-bacterial activities. Mosquito species are well known vectors for the transmission of vector borne diseases affecting human beings particularly malaria, dengue, yellow fever, Chikungunya fever and filariasis. In recent years development of new synthetic products and their introduction into international usage has become a very costly affair and shows side effects. Hence, a lot of time, money and resources are channelled towards the synthetic formulation. Different extracts of Annona squamosa were prepared and they are formulated as a paper coil and evaluated by using commercially obtained paper coil as a standard for its mosquitocidal...
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Posted by admin on Sep 30, 2016 in |
The objective of this study was to compare efficacy of 1% Alendronate gel and Tetracycline fibers as an adjunct to scaling and root planning (SRP). A total of 30 patients with diagnosis of chronic periodontitis (60 localized periodontitis sites) in an age group of 20-55 years were selected. None of these patients had received any non-surgical or surgical periodontal therapy and had periodontal pockets measuring 4–7 mm clinically. Two sites in any quadrants after randomization were selected in each patient. Navy Plaque index (Grossaman and Fedi) and Gingival bleeding index (GI) (Loe and Sillness), Clinical probing depth (PD) and Clinical attachment level (CAL) were measured at baseline 1,3 and 6 months. Random allocation of test site (treated with 1% Alendronate Gel) and control site (treated with Tetracycline fibers). The results revealed that there was significant improvement in all clinical parameters from baseline to 6 months and test group presented with better results than control group however the difference was not statistically...
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Posted by admin on Sep 30, 2016 in |
Tuberculosis (TB) remains a highly significant infection of microbial origin causing death of human in developing countries. The anti-tubercular drugs are less effective because of the emergence of multi-drug resistant strains and ineffective to extensively drug resistant strains of Mycobacterium tuberculosis. Plants are being an alternative source of anti-microbial compounds; the aim of this study was to investigate anti-mycobacterial potential of the few Indian medicinal plants and isolated compound from these to standard avirulent Mycobacterium tuberculosis H37Ra, standard virulent H37Rv and isoniazid resistant clinical isolates using Broth Microdilution Method. Methods and Results: Lyophilised ethanolic, aqueous and aqueous ethanolic extracts of four Indian medicinal plants viz. Coleus amboinicus, Glychirrhiza glabra, Pongamia pinnata and Terminalia chebula were screened for their in vitro antimycobacterial activity by Broth microdilution method (BMM) after 14 days of incubation at 37°C. The ethanolic extract of G. glabra was active against the tested strains of M. tuberculosis in primary screening with a MIC of 500µg/ml. The other extracts failed to exhibit inhibition at the tested concentrations. Glycyrrhizin...
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Posted by admin on Sep 30, 2016 in |
The natural acid like lemon juice provides mild acidic state which used to catalyzed reaction of primary amine and aldehyde in green solvent like ethanol at room temperature reflux for 1.5 hour gives Schiff bases in good yield. Schiff bases have attracted considerable attention of us due to their significant biological activities 1 like anticancer, antitumor, anti inflammatory agents 4, insecticidal, antibacterial 5, antituberculosis, antimicrobial 2-3, antifungal 5, anti-HIV and metal corrosion inhibition activities of Schiff bases, besides wide range of pharmacological activities. Natural acid is non-polluting and does not employ any lethal materials, quantifying if as a green approach for the synthesis of Schiff bases. Schiff bases also have several types of biological actions such as analgesic and antimalerial 6. They are working as Ligands for complexes of metal ions. Organosilicon complexes with Schiff bases are found to be beneficial in various agricultural applications like fungicides, bactericides, herbicides, acaricides and antifeedants 7. The metal complex of Schiff bases are also of principal significance in analytical chemistry and chemotherapy...
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