Posted by admin on Oct 31, 2016 in |
Anti-diabetic activity of hydro-alcoholic extract of Chrysophyllum cainito frutis (CCE) was investigated against experimentally induced diabetics in rats using alloxan and streptozotocin (STZ). Acute toxicity study was performed and hydro-alcoholic extract of CCE was found to be safe at a dose of 2000 mg/kg bodyweight. Two doses 200 mg/kg and 400 mg/kg b.w p.o. of the CCE were subjected for the evaluation of anti-diabetic activity against the diabetic induced by alloxan (100 mg/kg, i.p) and STZ (50 mg/kg, i.p) in rats. Glibenclamide (5 mg/kg p.o) was served as standard in both the models. Fasting blood glucose, serum total cholesterol, serum triglycerides, lipid profile (HDL and LDL) and histopathology were evaluated in the study. Both the lower (200 mg/kg) and higher dose (400 mg/kg) of CCE showed a dose dependent significant decrease in blood glucose level, triglyceride, cholesterol levels and LDL and an increase in HDL in the treated diabetic rats when compared with diabetic control. Histopathology of pancreas showed regeneration of β-cells in extract treated diabetic rats. The results...
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Posted by admin on Oct 31, 2016 in |
Estimation of binding affinity of receptor – ligand complex in docking study of small molecules plays an Important role in structure based drug development process. The γ-amino-butyric acid amino-transferase (GABA-AT) exhibits a catalytic role in regulating γ-amino -butyric acid (GABA) level in brain and responsible for many physiological and pathological changes. Here, in – silico study of benzothiazoles – GABA analogs as γ-amino-butyric acid amino-transferase inhibitors for antiepileptic activity was done. Docking study was completed by AutoDock Vina 1.1.2 (MGI Tools 1.5.6) and PyMol 1.7. We derived energy optimized pharmacophoric features for eighty five ligands and; compared with carbamazepine and vigabatrine. Docking results reveal that most of the ligands showed more affinity (-10.6 to -6.2 kcal/ mol) towards GABA – AT as compared to carbamazepine (-6.7 kcal/ mol) and vigabatrine (-5.0 kcal/ mol). Derivative with serial number 68 was found to possess the best binding affinity for GABA-AT with scoring energy as 10.6 kcal/ mol. Different descriptors were derived and analyzed for any violation of Lipinsky’s rule of five...
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Posted by admin on Oct 31, 2016 in |
Buccoadhesive tablets have long been employed to improve the bioavailability of drugs undergoing significant first pass hepatic metabolism. Prochlorperazine maleate is an anti-emetic drug. It was under goes extensive first pass metabolism resulting in an oral bioavailability of 0 to 16 % and it shows variable absorption from GIT. Hence in the present work Buccoadhesive bilayered tablets of Prochlorperazine maleate were prepared with the objective of avoiding first pass metabolism and controlling the release of drug for prolog period of time. Controlled release buccoadhesive bilayered tablets containing Prochlorperazine Maleate was prepared using a 32 full factorial design. Amount of HPMC K4M CR and Carbopol 974 P NF were taken as the formulation variables (factors) for optimizing Bioadhesive strength and percentage release of drug. The bilayered buccoadhesive tablets were evaluated for Physical characterization, Assay, Swelling index, Adhesion study, In-vitro residence time, Microenvironment pH, In-vitro drug release and In-vitro permeation. The formulation with 5 mg HPMC and 7.5 mg Carbopol was consider as a best product with respect to Adhesive strength,...
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Posted by admin on Oct 31, 2016 in |
The galls of Quercus infectoria Oliv. (Family: Fagaceae) are commonly known as “Manjakani” by Malaysian community. It is one of the most popular traditional medicines used by the females to help regression of prolapsed uterus. The aim of this study was to perform the proximate analysis and quantitative estimation of gallic acid in the methanol extract of locally available galls of Q. infectoria using HPTLC. The gall powder was subjected to the quantitative determination of moisture content, ash and extractive values according British Pharmacopoeia. Preliminary phytochemical studies were performed on different extracts to find out the nature of phytoconstituents they contain. Quantitative estimation of gallic acid in the methanol extract was assessed through denstiometric scanning using a TLC Scanner 3 (Camag, Switzerland) with win CATS software. The results of the preliminary phytochemical screening of various extracts revealed presence of steroids, triterpenoids, saponins, flavonoids, tannins and phenolic compounds, carbohydrates, gums and mucilages respectively in the galls. Amount of of gallic acid in the sample was found to be 0.218 ±...
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Posted by admin on Oct 31, 2016 in |
Lyophilization also known as freeze drying is a process in which water is removed from a product after it was frozen and placed under a vacuum, allowing the ice to change directly from solid to vapor without passing through a liquid phase. The process consists of three separate, unique, and interdependent processes; freezing, primary drying (sublimation), and secondary drying (desorption). Esomeprazole sodium for Injection 20 mg and 40 mg formulation was developed by optimized Lyophilization cycle. The objective of the research work was to compare the lyophilsation process cycle between Lab scale and scale up batches. The collapse temperature (Tc) for the product was identified using Freeze-drying microscope (FDM). During manufacturing the samples were exposed to above said collapse temperature to study the impact on product quality. Lab scale manufacturing was executed in Tofflon Lyophilizer 0.5 and scale-up batches were executed in Tofflon Lyophilizer 13. The Lyophilisation cycle and analytical results for both the batches were...
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