Posted by admin on Nov 30, 2016 in |
The methanolic extract (ME) of leaves of Ficus religiosa (Family: Moraceae) was evaluated for their wound healing activity in rats. It was carried out in rats by employing excision wound models following topical application. The qualitative preliminary phytochemical analysis of ME indicated the presence of tannins, triterpenoids, alkaloids and steroids. All healthy animals of either sex were grouped into four groups and each group contains six rats. The rats of ointment (5 and 10 %w/w) of ME treated were compared to povidone-iodine ointment treated as a standard. The results of present work revealed that the 10%w/w ointment of ME of Ficus religiosa treated wounds showed significant reduction of wound contraction area as compared to control and indicated rapid...
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Posted by admin on Nov 30, 2016 in |
Mangrove survives in the most hostile environment with fluctuating tidal and saline regime. The present work focused on a detailed study on quantitative estimation of phytoconstituents and DPPH radical scavenging activity of Excoecaria agallocha L., a mangrove from Kattiparambu and Chirackal of Ernakulam District, Kerala. Preliminary phytochemical investigation indicated the presence of biologically active metabolites of leaf, stem and root of the plant in different solvents. Methanol and hexane gave maximum results. Since the plant grows in stress conditions showed the presence of amino acids. Recorded values for high protein in leaf (4.8%), stem (1.93%), Carbohydrate in the root (11.17%) at Chirackal. Phenols (6.02%), flavanoids (3.6%) and tannins (5.68%) was observed in leaf samples of the species at Chirackal. Methanolic extract showed maximum free radical scavenging activity (IC50-141.56µg/ml) in leaf samples and stem showed minimum (IC50-931.3µg/ml) in chloroform extract. The range of DPPH radical scavenging activity in the solvent is Methanol > Ethyl acetate > Ethanol >...
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Posted by admin on Nov 30, 2016 in |
Previous studies have demonstrated that the activation of adenosine A2B receptors increases adenosine 3′-cyclic monophosphate (cAMP) accumulation in rat skeletal muscle cells, which is probably an important yet unknown mechanism contributing to the regulation of skeletal muscle functions. via rat L6 skeletal muscle cells, it has been elucidated further potential molecular signaling responsible for adenosine A2B receptor modulations via quantitative real-time PCR assays (probe-based). The results of the present study has shown for the first time that NECA alters the expression of PGC-1α significantly in both one week and 19 hours starved skeletal muscle cells (P<0.05 and P<0.01, respectively) (2.5 and -0.58 fold change compared to vehicle, respectively). Adenosine A2B receptors mediate NECA-modulated PGC-1α mRNA gene expression in skeletal muscle cells. To our knowledge, this is the first study demonstrating an induction of PGC-1α gene by CGS 21680 significantly (P<0.001) (around 1.8 fold change compared to vehicle). In the current study, NECA (10 μM) increases NR4A1 and NR4A3 mRNA gene expression significantly (P<0.05) (around 2.7 and 5.2 -fold change...
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Posted by admin on Nov 30, 2016 in |
Adenosine is a strong modulator of gene expression, which underpins some of its potential expected effects in skeletal muscle in terms of fatty acid metabolism. Accumulating evidence highlights a critical role for the adenosine system in the regulation of fatty acid metabolism and obesity. However, its molecular signaling role in fatty acid transport and oxidation in skeletal muscle is less clear. The aim of the present study was to examine whether acute and chronic treatment of skeletal muscle cells in vitro with stable adenosine analogue NECA, selective adenosine A2B receptors antagonist/inverse agonist PSB 603, and/or selective adenosine A2A receptors agonist CGS 21680 affects mRNA expression of key metabolic genes in skeletal muscle. Rat L6 skeletal muscle cells were treated with NECA, PSB 603 and CGS 21680 for determination the mRNA expression of genes involved in fatty acid transport and oxidation (CPT1B, ACCβ and LCAD) using QRT-PCR (Probe-based). The results of the present study have shown for the first time that NECA alters the expression of a number of genes...
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Posted by admin on Nov 30, 2016 in |
In present investigation of some ethyl1-((5-amino-1, 3, 4-thiadiazol-2-yl) methyl) – 5 – ethyl-2, 6-dimethyl – 4 – phenyl-1, 4-dihydro pyridine-3-carboxylate are designed and docked active site of cavity 1# of open channel voltage gated calcium channel protein (3DVE). The compounds evaluated in silico (docking) to distinguish their hypothetical binding mode using the X-ray crystal structure of Ca2+/CAM-CaV2.2 IQ domain complex 3DVE obtained from protein data bank as target protein Respectively. In this docking studies carried out the comparative docking experiments of designed compounds with known calcium blockers Ethosuximide, gabapentine with dock score calculated -4.9318,-4.6489 respectively. Obtained results were evaluated in terms of dock score into the active site of receptor. One of the conformers of compound Cl 123(C1)and AN 123 (C15),CH=CH 123 (C6),Fur 123 (C9) found to have lowest dock scores-3.2496 and -3.1749 , -2.8439, – 2.6359 respectively and said to have more affinity for active site of Ca2+/CAM-CaV2.2 IQ domain complex receptor than other molecules. More the negative value of the energy of binding the better is affinity...
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