Posted by admin on Sep 30, 2016 in |
The Goal of the present investigation was to design and evaluate gels for topical delivery of water insoluble antifungal agent Fluconazole with an aim to increase its penetration through skin and there by its flux. This is a broad spectrum imidazole derivative useful in the treatment of superficial and systemic fungal infections. The solubility of Fluconazole is increased by preparing solid dispersions with using mannitol, urea, polyethylene glycol 6000, polyvinyl pyrrolidone K30 and ß-cyclodextrin as carrier. Solid dispersion of Fluconazole was prepared by physical mixture method, solvent evaporation method, fusion method, Kneading Method and complex formation, in-vitro release profiles of all solid dispersions were comparatively evaluated and also studied against pure drug of Fluconazole. Faster dissolution was exhibited by solid dispersion containing 1:3 ratio of drug: mannitol by fusion method. The prepared solid dispersions were subjected for percent practical yield, drug content, infra red (I.R.) spectroscopic studies and differential scanning calorimetry (DSC). FT-IR spectra revealed no chemical incompatibility between drug and mannitol. Drug- polymer interactions were investigated using differential...
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Posted by admin on Sep 30, 2016 in |
Candesartan Cilexitil is an angiotensin II receptor antagonist used mainly for the treatment of hypertension. Candesartan cilexitil is a BCS class II drug having low solubility and high permeability. Candesartan Cilexitil is a prodrug which is rapidly converted to the active drug, Candesartan, by ester hydrolysis during absorption from the gastrointestinal tract. The bioavailability of Candesartan Cilexitil is approximately 15% after an oral administration. To increase bioavailability of such drug, solubility and dissolution are important parameters. To increase solubility of candesartan cilexitil, spray drying technique was incorporated with use of Fluidised Bed Processor. Also dissolution rate was improved with the use of appropriate superdisintegrants. Superdisintegrants used are Croscarmellose sodium, Crospovidone and Sodium starch glycolate. Dissolution rate of Candesartan Cilexitil from prepared dispersible tablet was compared with marketed formulation. Results have shown that solubility of Candesartan Cilexitil has been increased and dissolution rate was improved significantly. The transformation of Candesartan Cilexitil from crystalline to amorphous state by spray drying and the use of superdisintegrants are considered among the factors which...
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Posted by admin on Sep 30, 2016 in |
Affordability to healthcare is one of the major factors that have impact on the health care access in the Indian subcontinent. It is the main responsibility of the pharmacist in ensuring that patients get the right drug in the right quantity at the right time. Thus, such prescription pattern monitoring studies can promote appropriate use and reduction of abuse or misuse of monitored drug. The aim of such studies is to facilitate the rational use of drugs in the population. Irrational use of medicines leads to increased incidence of treatment failure, antimicrobial resistance and economic burden on the patient and the community as a whole. This is a descriptive observational prospective study in monitoring the drug prescription pattern in an ENT outpatient department. The objective of this study is to evaluate the patterns of drug prescribing practised. The study conducted observed that the drug prescription pattern was not as per the WHO guidelines and its indicators. ENT disorders led to school absenteeism as most of them where school going...
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Posted by admin on Sep 30, 2016 in |
The present study aimed to compare the effect of Omega3 fatty acids (OFA) and bone marrow mesenchymal stem cells (BMSC’s) on stress induced neuronal degeneration in CA1 hippocampus region of rats. For isolation and culture of BMSCs, a three week old Wistar rat was sacrificed, femora and tibiae were collected then mesenchymal cells cultured. The 6th passage cells are analyzed by flow cytometry for the expression of CD44, CD90 for positivity and CD45 for negative expression. In this experiment, thirty six rats aged 8 weeks old were divided into six Groups (n=6). Group I was control, Group II was given forced cold water swim stress, Group III: cold stress followed by saline Group IV and V, cold stress followed by oral gavages of omega-3 fatty acids 60mg/Kg for 2 weeks (short-term model) and 4 weeks (long term model) respectively and Group VI was cold stress followed by intravenous injection of 1 X 106 BMSCs. Behavioural function test performed using Y-maze. After treatment, rats were sacrificed for Cortisol assay and...
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Posted by admin on Sep 30, 2016 in |
A novel reverse phase liquid chromatographic method has been developed for the simultaneous estimation of Atorvastatin Calcium, Ezetimibe and Fenofibrate in bulk and Pharmaceutical formulations by using reverse phase Agilent 100-5 C18 column [250mm x 4.6mm]. The mobile phase (Methanol: water) in the ratio of 70:30% v/v was pumped at a flow rate of 1ml/min and the column effluents were monitored at 250nm using Variable Wavelength UV detector. Linearity was obtained in the concentration range of 20-100 µg/ml for Atorvastatin and Ezetimibe and 50-250µg/ml for Fenofibrate. The established method was statistically validated according to the ICH Q2B guidelines and the percentage relative standard deviation for precision, robustness and ruggedness was found to be less than 2% indicating high degree of precision and robustness. The percentage recovery for the accuracy was found to be 100.52%, 100.45% and 101.31% for Atorvastatin, Ezetimibe and Fenofibrate respectively which were within the specified limits of recovery. Assay for the marketed formulation proved that 99.86% of Atorvastatin, 99.61% of Ezetimibe and 99.83% of Fenofibrate. Hence...
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