Posted by admin on Aug 31, 2016 in |
Let G=(V,E) be a simple connected molecular graph in chemical graph theory, where the vertex set and edge set of G denoted by V(G) and E(G) respectively and its vertices correspond to the atoms and the edges correspond to the bonds. A topological index of a graph G is a numeric quantity related to G which is invariant under automorphisms of G. In this paper, the vertex Szeged index of Titania carbon Nanotubes TiO2(m,n) is...
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Posted by admin on Aug 31, 2016 in |
Male Wistar rats were procured from ‘The Bombay Veterinary College’, Parel, Mumbai. The animals were maintained and housed in cages and were acclimatized in laboratory conditions for ten days prior to the experiment. The experimental rats were fed orally with isoniazid 300 mg/ 70 kg body weight / day; rifampicin 450 mg / 70 kg body weight / day and Aloe vera extract 50 mg/ kg body weight / day for 30 days. Blood samples of the above groups (A to H) were taken after 30th day from cardiac puncture for estimation of mean concentration of complete blood Count. The present changes resulted from the antitubercular drugs in the hematological changes might be attributed to the toxic metabolites of the isoniazid and rifampicin. The toxic, reactive metabolites of the drugs bind to cellular macromolecules and release or form toxic free radicals intern caused the tissue damage. The present study was concluded that Supplementation of Aloe vera extract resulted in significant improvement in the hematological parameters might be due to...
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Posted by admin on Aug 31, 2016 in |
A new, simple, accurate, linear, precise, efficient and reproducible Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) method for the simultaneous determination of Ambroxol and Doxofylline in combined tablet dosage form. The chromatographic method was standardized using a Kromosil C18, 150mm × 4.6 mm, 3.5 ? (particle size), Thermo scientific from Germany with isocratic conditions, and mobile phase containing potassium dihydrogen orthophosphate buffer-pH 6.8 (0.01M KH2PO4): acetonitrile (25: 75) at flow rate of 1ml/min using UV detection at 257 nm. The retention times of Ambroxol and Doxofylline were 5min and 2min, respectively. The method was linear over the concentration range for Ambroxol 0.6–36 ?g/mL and for Doxofylline 16-96 ?g/mL. The recovery of Ambroxol and Doxofylline was found to be in the range of 98.13–99.85% and 98.09–99.66%, respectively. The validation of method was carried out using ICH guidelines. The described HPLC method was successfully employed for the analysis of pharmaceutical formulations containing combined dosage form. From the comprehensive validation conducted, it was concluded that the method is stable and could be used...
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Posted by admin on Aug 31, 2016 in |
Salacia reticulata, used in traditional and local medicines in Western Ghats of Southern India for the treatment of hyperglycaemia, is widely used for its sugar lowering activity. Accordingly, the present study was designed to investigate the possible actions of ethanolic extract of roots of Salacia reticulate (EESR) and an ethanolic extract of local formulation (EELF) made by mixing roots of Salacia reticulata along with five other drugs, each one in equal ratios, namely heartwood of Pterocarpus marsupium, stem of Tinospora cordifolia, fruits of Phyllanthus emblica, roots of Vetiveria zizanioides, and heartwood of Acacia catechu, on sugar lowering effect of diabetic rats. In the first set of experiments, hypoglycaemic effects of oral administration at various doses (50 and 100mg/kg) of the both extracts (EESR & EELF) were examined in normoglycaemics, glucose-induced hyperglycaemic rats. Optimum effect was observed in all groups of animals with a dose of 100mg/kg of the extracts (EESR & EELF). In other part of the study, both extracts (EESR & EELF) of 100mg/kg was given to alloxan...
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Posted by admin on Aug 31, 2016 in |
Emergence of antibiotic resistance has become a serious problem worldwide. The extensive and uncontrolled use of antibiotics increases the number of multi drug resistant (MDR) bacterial strains. The major mechanisms that bacteria evolve to develop resistance against β-lactam class of antibiotics are the production of β-lactamases and expression of low affinity penicillin binding proteins (PBPs). These PBPs are involved in the final stages of peptidoglycan synthesis. Hence, PBPs are the primary targets for developing antibacterial agents as its inhibition leads to irregularities in cell wall structure and eventually cell death. Bioactive phytochemicals from medicinal plants have been proven to reveal important pharmacological and therapeutic properties for developing novel antibacterial agents. The current study epitomizes the binding of phytochemicals to the transpeptidation (TP) domain of PBP3 of E. coli, a major causative agent of bacterial infection. The phytochemicals exhibiting antibacterial activities were selected as ligands for docking studies employing Schrӧdinger Suite (Maestro, LLC, New York, NY, 2015). Amongst all the phytochemicals studied, chlorogenic acid (CGA) was found to have highest...
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