Posted by admin on Jul 31, 2016 in |
About 5 extract (hexane, acetone, ethanol, methanol, aqueous extraction) from the flowers of Mangifera indica was extracted. Ethanol extract was characterized by Gas Chromatography-Mass Spectroscopy. Seven constituents from 7 peaks were identified. Icosanedioic acid monomethyl ester, nonadec-16-enyl-benzene, 1, 9 diphenyl nonane, icosane, octadecane, dodecanoic acid butyl ester, tetracosyl- benzene were identified. The antimicrobial activity of different extract was tested against human and plant pathogenic bacteria. Ethanol extract showed significant role on inhibiting almost all tested pathogenic organisms and antitubercular activities at various...
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Posted by admin on Jul 31, 2016 in |
Back ground: Agomelatine is a novel antidepressant which acts through a combination of agonistic actions at MT1 and MT2 subtypes of Melatonergic receptors and antagonistic actions at 5-HT2C subtype of serotonergic receptors. Objective: The study was carried out to evaluate the efficacy and safety of Agomelatine in the treatment of Major depressive disorder. Method: A prospective study was conducted at outpatient department of Psychiatry, GSL Medical College & General hospital Rajahmundry, 76 outpatients between 18 to 65 yrs of age of either sex, who met DSM-IV-TR criteria for major depressive disorder and had a minimum depression score of 20 on HAM-D17 were assigned to 8weeks of treatment with Agomelatine (25-50 mg/day). The main efficacy outcome measured was HAM-D17 total score (change from baseline to last post-baseline assessment of 8 weeks). Secondary outcome measures were assessed on Clinical Global Impression- severity (CGI-S) and Clinical Global Impression–improvement (CGI-I) scales. Results: The study results showed that Agomelatine was effective in Major depressive disorder. HAM-D17 score reduced effectively from baseline to end of...
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Posted by admin on Jul 31, 2016 in |
Targeted genome engineering provides the ability to precisely modify genetic information in order to study gene function, biological mechanisms, and disease pathology. Historically, random mutagenesis or low-efficiency homologous recombination were used to modify the genomes of cell lines or animal models. However, new advances in the design of sequence-specific endonucleases have enabled more effective, targeted editing of the genome. The most recent and fastest growing method for genome editing is based on the Clustered Regions of Interspersed Palindromic Repeats (CRISPR) viral defense system found in bacteria and archaea. The CRISPR/Cas9 system is much easier to customize and optimize because the site selection for DNA cleavage is guided by a short sequence of RNA rather than an engineered protein as in the systems of zinc finger nucleases (ZFN), transcription activator–like effect or nucleases (TALEN), and meganucleases. Derived from a remarkable microbial defense system, Cas9 is driving innovative applications from basic biology to biotechnology and medicine. The simplicity of the CRISPR-Cas9 system has enabled its widespread applications in generating germline animal...
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Posted by admin on Jul 31, 2016 in |
The plant Maytenus emarginata belongs to the Family Celastraceae also known as Kankhera in Hindi have used effectively for the treatment of wide range of disorder as digestive ailments, nerve afflictions, Heart ailments, inflammation, nervous system, Rashes and as a tonic and anti stress. The present investigation is planned to evaluate safety and reversible contraceptive efficacy of aqueous methanolic extracts of Maytenus emarginata leaves to develop a orally effective and reversible male contraceptive. Animal were orally administrated with extract of Maytenus emarginata leaf at the dose of 50,100,200 mg/kg/ body wt/day per oral dose for 60 days. A marked dose dependent decrease in the count and motility of the sperms of treated rats was observed compared with control. Furthermore, pregnancy rate in females markedly reduced by 80% after the mating with male treated with 50,100,200 mg/kg Maytenus emarginata leaf extract. At the same time significant decrease were detected in seminal vesicles and testicular weight of rats that...
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Posted by admin on Jul 31, 2016 in |
The brachial plexus block(BPB) has gained importance as regional anaesthetic technique for upper limb surgery. The primary aim of our study was to evaluate the addition of intravenous dexmedetomidine before BPB on overall patient satisfaction and sucess with objective of comparing the onset and duration of block, haemodynamic stability and sedation score. A randomized double blind prospective study was carried out on seventy patients of ASA grade I to III of either sex aged between 18 – 65 years scheduled for upper limb orthopaedic surgeries under BPB Who were randomly allocated into two groups: Group D (Dexmedetomedine) received Inj dexmedetomidine 1 µg/kg in intravenous 100 ml saline and Group P (Placebo) received intravenous 100 ml saline started 10 min before block. Onset and duration of block, quality of block, Haemodynamic variables, time to first rescue analgesics in post-op period, Sedation score, VAS score and Incidence of side effects and complications were recorded. Statistical analysis was done using Graph pad software. Hemodynamic variables, sedation score and satisfaction scores were analyzed...
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