Posted by admin on Oct 31, 2016 in |
In this present study a novel, simple, economical, accurate and precise RP-HPLC method has been developed and validated for simultaneous estimation of aspirine and clopidogrel in combined tablet dosage form. Chromatographic separation was carried out on a Kromasil HD C18 (150 x 4.6 mm, 5µm) column with a mixture of acetonitrile and 0.1% (v/v) orthophosphoric acid aqueous solution in a ratio of 40:60 (v/v) as mobile phase at a flow rate of 1.5 ml/min. Detection was accomplished at 237 nm by employing a DAD (Diode array detector). The retention times were 2.78 and 4.99 min for aspirine and clopidogrel respectively. The method was validated according to ICH guidelines for accuracy, precision, linearity, specificity and sensitivity. Calibration plots were linear over the concentration range 10 – 70 µg/ml for both aspirine and clopidogrel with r2>0.997. Recovery was found to be 99.2 – 99.8% and 99.2 – 99.9% for aspirine and clopidogrel respectively with RSD < 2 for both. There were no interference due to any components of pharmaceutical dosage indicating...
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Posted by admin on Oct 31, 2016 in |
The cytotoxic effect of safranal, a pharmacologically active component of saffron, has been established in vitro. The aim of this study was to develop safranal nanoliposomes with a higher therapeutic index for the treatment of cancer. Thus, various PEGylated safranal nanoliposomes were prepared using HSPC and cholesterol by solvent evaporation. The liposomes were characterized by their size, in vitro cytotoxicity and in vivo therapeutic efficacy against C26 tumor bearing mice. Liposome characterization illustrated the size range of 140-230 nm and PDI of 0.2-0.3. The entrapment efficiency was considerably low due to the high instability of safranal in liposomes, causing a substantial in vitro release. In vitro cytotoxicity indicated higher toxic effects of safranal liposomes compared to free form. Treatment of tumor-bearing mice with selected safranal liposomes (50 mg/kg) did not improve the tumor size and survival of animals compared to controls. These results were presumably due to the physicochemical properties and dose dependent effects of safranal molecules. In addition, the intensive hydrophobic molecular interaction between safranal and cholesterol within...
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Posted by admin on Oct 31, 2016 in |
Paracetamol (acetaminophen) (PA) is a widely used as an analgesic and antipyretic drug that is commonly available in without a prescription. Paracetamol has been used to treat many diseases such as headache, muscle aches, arthritis, backache, toothaches, colds, and fevers. It cures pain in mild arthritis but has no effect on the underlying inflammation and swelling of the joints. It has been reported as the common cause of drug toxic ingestion. These studies aim to compare the effect of some Paracetamol derivatives such Phenacetin, p-nitroacetanilide, p-bromoacetanilide and n-acetylanthranilic acid on liver structure to avoid Paracetamol cancer effect during its metabolism. From the histological point of view, it has been found that p-nitroacetanilide is the best alternative due to its metabolite...
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Posted by admin on Oct 31, 2016 in |
Long term administration of antiepileptic drugs has been found associated with development of hypothyroidism. But conflicting reports about effect on thyroid stimulating hormone (TSH) are available in literature and duration required to bring about this effect has also not been reported. Therefore, twenty five newly diagnosed adult patients with epilepsy were subjected to estimation of TSH before starting phenytoin and then at 2 and 6 months of phenytoin therapy. Phenytoin levels were also estimated using high performance liquid chromatography at 2 and 6 months and correlated with levels of TSH. The levels of TSH at 2 months of phenytoin therapy were found to be significantly increased as compared to baseline levels (p=0.000) and at 6 months (p=0.021) as compared to the levels at 2 months. A significant positive correlation was observed between the levels of phenytoin and TSH at 6 months (r=0.807, p=0.000) while the correlation was not found to be significant at 2 months duration. Thus, it may be concluded that there is a risk of development of...
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Posted by admin on Oct 31, 2016 in |
Nigella sativa L. is an annual herb and cultivated largely in the East Mediterranean region. Seeds used in traditional folk medicine for the treatment of various purposes in the systems of Unani, Ayurveda, Chinese and Arabic. Nigella sativa seed oil was isolated using soxhlet hexane extraction process. GC-MS analysis identified a total of 32 compounds among which 9-eicosyne (63.04%), linoleic acid (13.48%), palmitic acid (9.68%) were the major constituents. Saturated aliphatic fatty acid accounted 63.04% of the seed oil extract. Fatty acid and monoterpene hydrocarbon constituted 23.26% and 4.91% respectively. Also the seed oil included compounds of alkanes and sesquiterpene hydrocarbons that constituted 2.84%, and 0.30% respectively. The seed oil was estimated for its chemical compounds and antioxidant activity using in vitro assays such as DPPH, ABTS, nitric oxide, hydrogen peroxide and total antioxidant scavenging capacity. Higher antioxidant scavenging activity of TAC and ABTS was found in seed oil. The seed oil contains higher percentage of fatty acids and exhibit antioxidant activity which are useful for preparation of pharmaceutical...
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