Posted by admin on Oct 31, 2016 in |
The antidyslipidemic and antioxidant activities of Allium sativum (Garlic) and Aloe vera (Ghee- kuwar) extracts have been studied in two models of hyperlipidemia, triton and cholesterol rich high fat diet induced hyperlipidemia. A. sativum, A. vera and Gemfibrogil were macerated with 0.2% aqueous gum acacia and the suspension was fed orally to rats of group III, IV and V at a dose of 400mg, 1g/Kg, and 50 mg/Kg (b.w.p.o.) respectively with triton. Serum lipids were found to be lowered by Allium sativum and Aloe-vera in triton induced hyperlipidemia. On the other hand chronic feeding of these extracts to animals in cholesterol rich high fat diet induced hyperlipidemia for 30 days caused lowering in lipid and protein levels of β lipoproteins followed by an increase in α lipoproteins. The results of present study demonstrate that hypolipidemic activity of these extracts mediate through inhibition of hepatic lipid synthesis, increased faecal bile acid excretion and induced plasma LCAT activity in rats. Furthermore these extracts (100 and 200 µg/ml) inhibited the in vitro...
Read More
Posted by admin on Oct 31, 2016 in |
The objective of the study is to measure the difference between the levels of LDL by Direct method versus Friedewald equation. The study includes 30 patients and 30 controls in the age of 25 – 75 years of both sexes. Fasting blood samples were collected and estimated Total Cholesterol (TC), Triglycerides (TG), Low Density Lipoprotein (LDL-C) cholesterol and High density lipoprotein (HDL) cholesterol, LDL cholesterol by direct method and by Friedewald’s formula (FW). There is a significant difference between LDL Direct and LDL-FW at triglyceride range of 1 – 100, (p= 0.01), 201 – 300 (p= 0.01) and no significant difference (p= 0.9) at 101 – 200, (p= 0.3) at 301 – 400 and (p= 0.2) at >400. There is a significant difference between LDL Direct and LDL-FW (p= 0.01) at total cholesterol range of 200 – 249 and no significant difference (p= 1.0) at 100 – 149, (p= 1.0) at 150 – 199 and (p= 0.9) at >250. There is significant difference between LDL values by direct and...
Read More
Posted by admin on Oct 31, 2016 in |
Development of microbial resistance leads new drug discovery, modification of antibiotics, administration of two or more antibiotics, use of traditional medicinal plants and their combinations with antibiotics. In the present study, 72 medicinal plants extracts were screened to potentiate the antimicrobial with erythromycin and amoxyclave. Among all the medicinal plant, Colebrookea oppositifolia was selected to analyze the synergistic activity with erythromycin and amoxyclave. The class I synergism (increase in the zone of inhibition) was observed in petroleum ether extract of leaves and inflorescence of C. oppositifolia in combination with amoxyclave with the increased zone from 6±0.2 mm to 8±0.2 mm and 6±0.2 mm to 10±0.2 mm, respectively. The class I synergisms also exhibited in methanol leaf extract of C. oppositifolia in combination with erythromycin and amoxyclave against S. aureus. Interestingly, the methanolic leaf extract (alone) did not show any antibacterial effect against S. aureus, but in combination with erythromycin/amoxyclave enhanced the zone of inhibition from 3±0.1mm to 9±0.2 mm and 5±0.2mm to 9±0.2mm, respectively. Class II synergism (making drug...
Read More
Posted by admin on Oct 31, 2016 in |
Mangifera indica is commonly found in India, which contains exalted medicinal values. TLC bioautography help us to identify the bioactivity of separated molecules in less time. In this study, leaves of Mangifera indica was subjected for various solvent extraction, followed with phytochemical screening and TLC bioautography for antioxidant and antibacterial activity. Column fractionation was done for all the extracts and subjected for spot assay. The plant chosen for this study was found to have antioxidant and antibacterial...
Read More
Posted by admin on Oct 31, 2016 in |
Pathogenic bacterial infections are of serious clinical concern around the world. Indiscriminate use of antibiotics makes this situation beyond control. Hence, microbiologists are trying to find out the new and promising therapies against these bacterial infections. In the present research, combination therapy, which comprises extract of different medicinal plants and cow urine distillate, was used against six pathogenic gram positive bacteria. The efficacy of this combination therapy was evaluated by two methods: agar well diffusion method and XTT-colorimetric method. It has been demonstrated that CUD has profound synergistic effect on the efficacy of plants-derived extracts against the pathogenic bacteria at the specified level. The relative activity of different extracts with CUD as solvent medium was found to be increased (R.A.I= 1.61 to 2.13) against every bacteria of present study. MIC & MBC results were greatly improved (upto 2 to 3 folds) by the combination therapy. The results obtained in the present study could be useful for further research to assess the effect of combination therapy against other pathogenic microorganisms...
Read More
