Posted by admin on Sep 30, 2016 in |
The purpose of this study examine whether the polymorphism in p53 upregulated modulator of apoptosis (PUMA) gene consequential treated with purified Nigella sativa lectin in tumor induced mice. Nigella sativa (N. sativa) is a widely used medicinal plant throughout the world. Possess a seed lectin that was purified by combination of ammonium sulfate precipitation and affinity chromatography on a Sepharose 4B column. The Nigella sativa strongly agglutinated human erythrocytes. The hemagglutinating activity of lectin was maintained after incubation at a wide range of temperature and pH and also was independent of divalent cations. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, lectin exhibited an electrophoretic profile consisting of a single band with apparent molecular mass of 45 kDa. Experimental groups were organized into 6 groups including 10 animals per each, additionally control group, blood samples were collected form mice before and after lectin treatment for liver and kidney enzymes biochemical analysis, Data statistical analysis for mitochondrial enzymes in our study showed a very highly significant (P>0.001) difference in the mean value...
Read More
Posted by admin on Sep 30, 2016 in |
Thiamine hydrochloride, also known as Vitamin B1, is a water-soluble vitamin which is important biologically and pharmaceutically. A new simple and sensitive spectrophotometric method has been developed for the determination of Thiamine Hydrochloride (Vitamin B1) in pure form and biological fluids. The method is based on the diazotization reaction of Thiamine Hydrochloride with Procaine HCl which reacted with sodium nitrate and hydrochloric acid to form a red water-soluble azo dye in basic medium, that has a maximum absorption at λmax = 507 nm. Beer’s law is obeyed over the concentration range (2-60 µg.ml-1), the molar absorptivity of 4.047×104 L.mol-1 .cm-1 and Sandell sensitivity of 7.417×10-3 µg .cm-2 and the method have an accuracy and precision between (0.02-0.16) while the relative standard deviation between (0.805-2.049). The structure of dye showed that (1:2) product was formed between the drug and diazotized reagent. The method was applied successfully for the determination of Vitamin B1 in pharmaceutical preparations and biological samples. The results showed that the concentration of vitamin B1were decreased, in both...
Read More
Posted by admin on Aug 31, 2016 in |
Many antifungal drugs are used topically in order to treat fungal diseases. Among them Ketoconazole is used widely among these topical antifungal drugs for eradication of dandruff. Scalp with dandruff causes more hair fall than normal scalp and hair fall is treated by topical Minoxidil formulation. Aim of present work is to prepare a topical formulation containing both ketoconazole and minoxidil in order to treat dandruff and associated diffused alopecia simultaneously. Both the drugs are incorporated in emulgel at a particular step in order to prepare formulation. Emulgel i.e. emulsion in gel have been emerged out as one of the most beneficial topical drug delivery system as it contain both hydrophilic and lipophilic drugs with dual controlled release system i.e. emulsion and gel. Incorporation of emulsion in gel also increases the stability of emulsion. Emulgel was prepared by using carbopol 934 as gelling agent. The influence of concentration of gelling agent and emulsifying agent on the drug release from the prepared emulgel was investigated using a 2×22 factorial design....
Read More
Posted by admin on Aug 31, 2016 in |
Flouroquinolones (FQ) used commonly for treatment of community and hospital-acquired infections, has been prefixed with multiple adverse effects. Amongst them the glycemic variability has been attention seeking. Drug induced dysglycemia can be acute or persistent and severe too. The present study is designed to evaluate and compare the actions of Levofloxacin vs. Norfloxacin in altering blood glucose levels in Alloxan induced diabetic albino rats. 30 albino rats with fasting blood sugar (FBS) in the range of 80-115 mg/dl were induced diabetes with intraperitoneal Alloxan (150mg/kg body wt). After 7 days of induction, the rats that developed a stable hyperglycemia with FBS≥250 mg/dl were selected for the study. Following administration of test and standard drug FBS levels were measured on days 0, 3, 7, 14 & 28 using glucometer. Statistically, values in all the groups were analyzed by using one-way analysis of variance (ANOVA), followed by independent T test for group comparisons. P values less than 5% (0.05) was considered as significant. Glycemic variation was found to be statistically significant...
Read More
Posted by admin on Aug 31, 2016 in |
Background-In September 2012, the world health organization reported a global prevalence of diabetes exceeding 300 million people, predicting a further 60-70% increase by the year 2030, which means India alone will have 100 million people by year 2030. Wound healing is problematic in diabetic patients. Encouraging results have shown that PDGF application is better than good wound care alone. But the evidence to demonstrate the safety and efficacy of PDGF in Diabetic ulcer is scanty. Aim-To Study the Efficacy of Platelet Derived Growth Factor in Diabetic Lower Extremity Ulcer. Methods-This study was a prospective, double blind, and placebo controlled study. A total of 29 patients with 35 ulcers were included and divided in case and control group. The study medication was administered in conjunction with standardized good wound care for 24 weeks or until target ulcer healed. Results-It was found that 74.2% of ulcers were neuropathic while 48.3% patients had foot deformity in this study. At the end of 24 weeks 100% ulcers in group receiving PDGF completely healed...
Read More
