Posted by admin on Mar 31, 2016 in |
Plants served as potent sources of bioactive compounds since ages. The understanding of phytochemical constitution of each and every plant on earth is the preliminary and the most important step in drug discovery. As Operculina turpethum(L.) Silva Manso is traditionally used for various ailments, the understanding of phytochemical composition of the plant is very essential. The present study was an attempt to identify the phenolic compounds present in the leaf of the plant O. turpethum. Total phenol and flavonoid content was determined from the methanol extract of the leaf and the extract was subjected to HPLC analysis with seven standard compounds. A gradient mobile phase comprising acetonitrile and 0.1% phosphoric acid was used to get the phenolic profile. The quantity of each compound present in the extract was calculated from the peak area and expressed in mg/ ml of sample injected. The first mobile phase revealed the presence of compounds viz., coumarin, ferulic acid, catechin, salicylic acid, rutin, vanillin and gallic acid. Among the seven identified phenolics, coumarin was...
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Posted by admin on Mar 1, 2016 in |
In the present study, total fifteen compounds of 1,2,4,5-tetraoxane derivatives were docked. Two series of 1,2,4,5-tetraoxane derivatives were taken for molecular docking studies, one tetraoxaquines, a hybrid of two pharmacophores such as 4-aminoquinoline & 1,2,4,5-tetraoxane, and other dispirotetraoxanes. The docking studies were performed into the binding pocket of a falcipain-3 protein (pdb: 3bwk – hydrolase) by using the Ligand fit module within docking server. The results showed a better binding affinity of hybrid tetraoxaquines compared to dispirotetraoxanes at the active site of falcipain-3 because of very low binding energies for falcipain-3 protein (pdb: 3bwk – hydrolase). Therefore, hybrid tetraoxaquines are better Cysteine proteases (falcipains) inhibitors. They would be potent antimalarial agents. So the proposed inhibitors in the future could be more effective to treat...
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Posted by admin on Mar 1, 2016 in |
Organic solvents are integral part of chemical synthesis in pharmaceutical industry. These are commonly used as reaction media, in separation, purification of synthetic products and also for cleaning of equipment. Some of the solvents degrade to other solvents or unknown impurity either during synthesis or in analytical conditions. In this study, we have investigated formation of a trace level impurity, its identification and quantitation by successfully applying the concept of relative response factor (RRF) in GC-HS. The trace level impurity was identified as dimethyl ether (DME), formed due to interaction of strong acid and methanol. Unavailability of its commercial standard made the quantitation in drug substance challenging using gas chromatography. In this work, we have extended the concept of RRF for determination of DME in drug substances. The RRF of DME was established against other process solvents used in method of analysis. Application of RRF in quantitation of DME eliminates the requirement of its external standards during routine analysis at quality control...
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Posted by admin on Mar 1, 2016 in |
The package inserts (PI) are a valuable source of information on drugs for physicians and patients. However, the degree of awareness of PI & their perceptions at both physician and patient front has not been studied in India in current times. The study was undertaken to assess the degree of awareness regarding information in drug package inserts at a tertiary care centre in western India. A questionnaire based survey was conducted among 100 physicians and 100 patients. The questionnaire mainly focused on the level of awareness, understanding and utility of information given in PI. The responses were collated and analyzed. The results showed that 20% of the physicians admitted that they rarely or never referred to PI. 30% did not consider the PI to be important in their clinical practice & 18% did not feel the need for PI to be always available. 64% opined that the language was too technical for comprehension by lay persons. Only 10% said that they always asked their patients to read the PI....
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Posted by admin on Mar 1, 2016 in |
Terminalia paniculata Roth. (TP) is a tropical tree. Traditionally, flower juice and bark of Terminalia paniculata have been used as a remedy for cholera, for the treatment of diabetes, inflamed parotid glands and in menstrual disorders. The objective of the present study is to evaluate the antidiabetic potential of Terminalia paniculata bark extract in various in-vitro models and its safety evaluation using Wistar albino rats. The in-vitro antidiabetic activity was studied using Alpha amylase, Alpha glucosidase inhibition and Glucose diffusion assay. The nitric oxide radicals scavenging activity was determined using antioxidant assay. Acute and sub acute toxicity studies were designed as per the OECD guidelines- 423. TP extract shows maximum inhibition of 94.5 % on α-Amylase at 700 µg/ml and 94 % on α-Glucosidase at 1000 µg/ml. In glucose diffusion, it showed highest activity at a concentration of 1500 µg/ml. Also, it had excellent nitric oxide scavenging activity and maximum percentage inhibition of 86.12±1.01 was observed at 1500 µg/ml. Acute toxicity study supported the tolerance level even at the...
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