Posted by admin on Apr 30, 2016 in |
The aim of this study is to investigate the cytotoxic effect of various extracts from Phyllanthus emblica L. According to Ayurveda this plant has potent anticancer activity and hence, this study is targeted at screening potential cytotoxic extracts using a yeast system. Yeast has been used as a model organism for many preclinical drug screens. In this study phytochemical extraction has been done by three methods (sonication, low and elevated temperature systems) using six different solvent systems (aqueous, methanol, ethyl acetate, petroleum ether, chloroform: methanol and water: methanol). The viability studies have been done using Methyl Thiazole Tetrazolium (MTT) assay. Aqueous extract at elevated temperature and water: methanol extract showed viability less than vinblastine. Further phytochemical analysis was done to identify major group of compounds present in each extract. It was concluded that flavonoids are present in extracts by doing TLC. HPTLC using standards was carried out and showed that at least two chemotherapeutic agents kaempferol and rutin were present. The data further indicates that kaempferol is major constituent...
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Posted by admin on Apr 30, 2016 in |
Cucumis callosus (Rottl.) Cogn. (Cucurbitaceae) is a highly branched very common prostate, perennial herb, distributed throughout India in the arid zones. Objective: To evaluate the antihyperglycemic potential of C. callosus fruit in experimental animal model. Materials and methods: Hyperglycemia was induced in rats by single intraperitoneal injection of STZ (55 mg/kg body weight). Three days after STZ induction, the hyperglycemic rats were treated with MECC at the doses of 200 and 400 mg/kg body weight (p.o.) daily for 15 days. Glibenclamide (0.5 mg/kg, orally) was used as reference drug. The fasting blood glucose levels were measured on every 5th day during the 15 days of treatment. Serum biochemical parameters including lipid content were estimated. Results and discussion: MECC at the doses of 200 and 400 mg/kg significantly (P < 0.01) and dose dependently reduced blood glucose levels towards normal as compared to that of STZ control group; the higher dose (400 mg/kg) being the most potent showing complete normalization of blood glucose levels. Serum biochemical parameters including lipid profile...
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Posted by admin on Apr 30, 2016 in |
Six new Phthalic acid amides of the general formula C8H5O3-NH-R were prepared by the reaction of Phthalic anhydride with various amines namely Glycine, N, N-diphenyl amine, 1-naphthyl amine, 4-amino toluene-3-sulphonic acid, Toluene-p-sulphonamide, m-nitro aniline. The synthesized compounds were characterized by using IR, 1HNMR & 13CNMR spectroscopy. Petra Osiris Molinspiration (POM) analyses revealed the significant bioactivity by elucidating the structural parameters in drug design of the synthesized compounds. In this work we have performed POM of our synthesized compounds to predict their drug like behaviour. Petra program package was used for the calculation of physiochemical properties of the organic compounds. On the basis of findings of Petra analyses reveals that our synthesized compounds have potential pharmacophores for antiviral and anti bacterial activities. Prediction of bioactivity by Molinspiration software calculates druglikeness score against GPCR ligands, ion channel modulators (ICM), kinase inhibitors (KI), nuclear receptor ligands (NRL), protease inhibitors (PI) and other enzyme inhibitors (EI). All synthesized compounds showed better activity against ICM, KI, PI and NRL as compared to the reference...
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Posted by admin on Apr 30, 2016 in |
Ascorbic acid is one of the essential and most vital medicines needed to maintain healthy living. Due to its non-availability through endogenous biosynthesis in humans, it is arguably one of the most consumed Over-The-Counter drugs. Thus, a simple, precise, low-cost and accurate reversed-phase liquid chromatographic (RP-LC) method was developed and optimized to analyse for the percentage content of ascorbic acid in different brands of Vitamin C syrups. The chromatographic separation carried out on an ODS (C18) Ultra sphere column, 5µm (25cm x 2mm), was achieved with HPLC water and methanol as the mobile phase, pumped at a flow rate of 1ml/min and using an ultraviolet detector for detection. The calibration curve was linear over the range of 10-100µg/ml and the method was found to be specific as no interference peaks of impurities and excipients were observed. The method developed was used to assess the quality of fifty-five samples of different types of Vitamin C syrup. Among the samples assessed for their percentage content of ascorbic acid, 18.2% fell below...
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Posted by admin on Apr 30, 2016 in |
Objective: The present investigation is focused on detecting anti-carcinogenic potential of virgin coconut oil. Methods: The virgin coconut oil (VCO) from coconut endosperm was prepared by cold compression method. It was examined then for cytotoxicity by cell exclusion studies using trypan blue dye. Chemical carcinogenesis method using benzopyrene was used to study the anticarcinogenecity of VCO. Curcumin was taken as standard. The low dose and high dose VCO was compared with normal, vehicle and standard treatments. Biomarker studies and histopathological evaluation was also performed. Result: The VCO upto 9μl showed no significant cytotoxicity. The low dose (4ml/kg body wt) and high dose (8ml/kg body wt) of VCO had significant reduction in gastric Lactate Dehydrogenase (LDH) activity, which is a biomarker for gastric cancer. Histopathological reports also showed very less infiltrations and absence of hyperplasia compared to control. Conclusion: The present study showed that, the virgin coconut oil have anti-carcinogenicity property, thus it prevent hyperplasia in the stomach of mice, induced by...
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