Posted by admin on Aug 31, 2015 in |
A series of novel 2- substituted benzothiazole dithiomidocarbonate derivatives were synthesized by the reaction of 2-amino benzothiazole with various halides. The test compounds were screened for their antimicrobial activity (antibacterial and antifungal) by disc diffusion method and the results of the microbial activity indicates that the test compounds exhibited varying degree of antimicrobial activity. Among the substituents benzyl moiety exhibited the most potent activity and the compound Dibenzyl (1,3 benzothiazo-2yl) Dithioimidocarbonate(4T1) emerged as the most active antimicrobial agent and it is equipotent when compared to the reference standard Ciprofloxacin. Among the substituents propyl moiety exhibited the most potent activity and the compound Dipropyl (1,3 benzothiazo-2yl) Dithioimidocarbonate (4T3) emerged as the most active antifungal agent and it is equipotent when compared to the reference standard ...
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Posted by admin on Aug 31, 2015 in |
The present investigation was carried to determine UV-VIS, FTIR and GC-MS analysis of Ampelocissus latifolia tuberous root. The ethanol extract was examined under visible and UV light for the proximate analysis. The UV‐VIS profile of Ampelocissus latifolia tuberous root ethanol extract showed the peaks at 409 nm, 424 nm and 479 nm with the absorption of 1.682, 1.704 and 1.479 respectively. The FTIR spectrum was revealed the presence of alcohols, aromatic compound, alkanes, aldehydes, ketones, alkenes, amines, amides, nitro compounds, carboxylic acids, ethers, esters and alkyl halides in ethanol extracts of Ampelocissus latifolia tuberous root. GC-MS analysis of ethanol extract of Ampelocissus latifolia tuberous root was revealed the presence of 14 phytochemical compounds. The presence of various functional groups and phytocompounds in Ampelocissus latifolia tuberous root confirm that it act as a most important source of drugs against various...
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Posted by admin on Aug 31, 2015 in |
Alternanthera brasiliana is a medicinal plant used in the folkloric treatment of malaria, pains, infections and diabetes in South-western states of Nigeria. Recent reports indicate some central activities for the plant. This study evaluated ethanolic leaf extract of the plant for novelty-induced behaviour, anxiety, sedation and convulsion in mice. Dried powdered leaves of the plant were extracted with 70% ethanol, filtered and concentrated in vacuo to obtain ethanolic leaf extract (ELE) of A. brasiliana. The acute toxicity (LD50) of the ELE was determined through oral (p.o.) and intraperitoneal (i.p.) routes. ELE (250, 500 and 1000 mg/kg, p.o.) was evaluated for novelty induced behaviours, anxiolytic, sedative and anticonvulsant activities. The LD50 values obtained for the ELE were ≥5000 mg/kg, p.o. and 3808 mg/kg, i.p. The ELE (500-1000 mg/kg) caused significant (p<0.05) increase in rearing [p<0.05, p< 0.01; F(4, 25)= 37.0] and locomotor [p<0.05, p< 0.01; F(4, 25)= 36.0] activities; caused increase in the number of head dips at 1000 mg/kg [p<0.01, F(4, 25) = 9.0] and non-significantly increased time spent...
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Posted by admin on Aug 31, 2015 in |
The use of noninvasive biological samples such as saliva, it is of great interest for therapeutic drug monitoring (TDM), including the anticonvulsants Phenytoin (PHT). A simple analytical methodology by liquid chromatography-tandem mass spectrometry was developed and validated to carry out a relative comparative bioavailability (RBA) study of two PHT formulations in human plasma and saliva and the subsequent correlation between both matrixes. A single-dose, randomized-sequence, open-label, two-way crossover study, was conducted in 24 healthy Latin American male volunteers. The bioequivalence of 200 mg PHT tablet was evaluated using plasma and saliva to determine Cmax, Tmax, AUC0-t and AUC0-∞. Figures of merit of the proposed methodology were as follows: linear ranges of 40-5055 ng/mL and 5-1340 ng/mL for plasma and saliva, respectively, with a correlation coefficient of (r)>0.999. The LOD and LOQ were 15 ng/mL -40ng/mL for plasma and 1.5 ng/mL – 5.0 ng/mL for saliva. Accuracy, precision (as %CV) and recovery were accepted according the bioequivalence criteria. Stability showed %bias<15%. The PHT saliva/PHT plasma ratios for all parameters were:...
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Posted by admin on Aug 31, 2015 in |
Objective: This study was undertaken to investigate the potential role of etanercept in amelioration of myocardial I/R injury induced by ligation of coronary artery in a mice model. Materials & methods: Adult male Swiss Albino mice were randomized into 4 equal groups. Group (1) sham group: mice underwent the same anesthetic and surgical procedure as the control group except ligation of LAD coronary artery , Group( 2) control group: mice subjected to regional ischemia for 30 min and reperfusion for 2 hours by ligation of LAD coronary artery ,Group( 3) control vehicle group: same control group except mice given IP normal saline before reperfusion 5 minutes, Group( 4) Etanercept treated group : mice treated with etanercept 5 mg/kg i.p. 5 minuts before reperfusion of ligation of LAD coronary artery. The heart tissue (below the ligation site) was used for measurement of apoptosis (caspase 3 and Bcl-2)and histopathology study. Results: Histologically, all mice in control group showed significant (p<0.05) cardiac injury. Furthermore all mice in control group showed significant (p<0.05)...
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